Determination by HPLC of ascorbic acid and tocopherols in fruits
DOI:
https://doi.org/10.5433/1679-0359.2010v31n2p381Keywords:
Antioxidants, Quantification, ChromatographyAbstract
The fruits and vegetables are recommended for human feeding due to the richness of antioxidant compounds. The bioactive compounds from the diet, such as vitamins C and E are important to reduce the speed of initiation or prevent the spread of free radicals. The objective of the work was to evaluate the ascorbic acid and tocopherol content of selections of Pitanga (Eugenia uniflora L.), butiá (Butiá capitata), blackberry (Rubus spp), blueberry (Vaccium Reade ashei), loquat (Eribrotia japônica), jambolan (Eugenia jambolana), purple araçá (Psidium rufum), physalis (Physalis peruviana), pear (Pyrus communis) and peaches (Prunus persica). The fruits were from 2006/2007/2008 harvest, which were obtained in Pelotas (RS) region. The tocopherol analyses were performed according Rodrigues-Amaya (1999), and ascorbic acid according Vinci, Botre e Ruggieri (1995). The tocopherol and ascorbic acid identification and quantification was performed by high-efficiency liquid chromatography system (HPLC), using a fluorescent detector for tocopherol (excitation of 290 nm and emission of 330 nm) and a UV-Visible (254 nm) for ascorbic acid. The blackberry cv. Tupy showed the highest tocopherols content (8,251 ?g.g-1 fruit) when compared with the other fruit content. The tocopherol was not found in pears and peaches of cv. Sensação and cv. Granada. The ascorbic acid content ranged from 9,291 mg.g-1 fruit for the physalis to 0,013 mg.g-1 fruit for the jambolan. It was concluded that the fruits are good sources of bioactive compounds.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
