Inoculation methods and virulence of Beauveria bassiana (Bals.) Vuill. to Cosmopolites sordidus (Germar) (Coleoptera:Curculionidae) in laboratory

Authors

  • Ernesto Antonio Lema López Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Pedro Manoel Oliveira Janeiro Neves Universidade Estadual de Londrina
  • Vaneide Pimenta Almeida Uniersidade Estadual de Londrina
  • Giovanni Tamiozzo Universidade Estadual de Londrina
  • Marilene Francelli Centro Nacional de Pesquisas de Mandioca e Fruticultura

DOI:

https://doi.org/10.5433/1679-0359.2010v31n1p67

Keywords:

Biological control, Entomopathogenic fungi, Bioassay

Abstract

The research aimed to assess different inoculation methods of Beauveria bassiana (Bals.) Vuill. on Cosmopolites sordidus (Germar) adults, in laboratory conditions on an isolates selection bioassay. Conidial suspensions of UEL151 strain were applied on insects through the following methods: spray, immersion, dorsal and ventral topical. Low mortalities were achieved for all inoculation methods, with a higher value for immersion (21.7%) at 37 days after application (DAA), as the rest of methods were significantly lower. The evaluation must be extended to a maximum of 22 DAA because the sporulation rate (confirmed mortality / total mortality) decreased with time in all methods, with values of 95.8 and 75.3%, at 22 and 37 DAA, respectively, on the immersion method. Dorsal and ventral topical methods caused not significant C. sordidus virulence, so new tests are necessary with different fungus concentrations.

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Author Biographies

Ernesto Antonio Lema López, Conselho Nacional de Desenvolvimento Científico e Tecnológico

Eng. Agrônomo, Estudante Mestrado em  Agronomia/Entomologia/Controle Microbiano de pragas, Bolsista do CNPq-Brasil.

Pedro Manoel Oliveira Janeiro Neves, Universidade Estadual de Londrina

Eng Agrônomo, Doutor em Entomologia/Controle Microbiano de Insetos, Bolsista Produtividade do CNPq. Professor associado da Universidade Estadual de Londrina.

Vaneide Pimenta Almeida, Uniersidade Estadual de Londrina

Eng. Agrônomo.

Giovanni Tamiozzo, Universidade Estadual de Londrina

Aluno de agronomia. Estagiário PIBIC.

Marilene Francelli, Centro Nacional de Pesquisas de Mandioca e Fruticultura

Eng Agrônomo, Doutor em Entomologia/ Pesquisadora da Embrapa – Centro Nacional de Pesquisas de Mandioca e Fruticultura.

Published

2010-04-30

How to Cite

López, E. A. L., Neves, P. M. O. J., Almeida, V. P., Tamiozzo, G., & Francelli, M. (2010). Inoculation methods and virulence of Beauveria bassiana (Bals.) Vuill. to Cosmopolites sordidus (Germar) (Coleoptera:Curculionidae) in laboratory. Semina: Ciências Agrárias, 31(1), 67–74. https://doi.org/10.5433/1679-0359.2010v31n1p67

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