Effect of different gonadotropins on in vitro maturation of sheep oocytes
DOI:
https://doi.org/10.5433/1679-0359.2022v43n6p2731Keywords:
Follicle-stimulating hormone, Metaphase II. , Hoechst 33342, GSH.Abstract
The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 µg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes.
Downloads
References
Aghaz, F., Hajarian, H., Shabankareh, H. K., & Abdolmohammadi, A. (2015). Effect of sericin supplementation in maturation medium on cumulus cell expansion, oocyte nuclear maturation, and subsequent embryo development in Sanjabi ewes during the breeding season. Theriogenology, 84(9), 1631-1635. doi: 10.1016/j.theriogenology.2015.08.013 DOI: https://doi.org/10.1016/j.theriogenology.2015.08.013
Avelar, S. R. G., Moura, R. R., Sousa, F. C., Pereira, A. F., Almeida, K. C., Melo, C. H. S., Teles, A. C. A., Fº., Baril, G., Melo, L. M., Teixeira, D. I. A., & Freitas, V. J. F. (2012). Oocyte production and in vitro maturation in Canindé goats following hormonal ovarian stimulation. Animal Reproduction, 9(1), 7-32.
Blaha, M., Prochazka, R., Adamkova, K., Nevoral, J., & Nemcova, L. (2017). Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B). Prostaglandins Other Lipid Mediat, 130,38-46. doi: 10.1016/j.prostaglandins.2017.04.001 DOI: https://doi.org/10.1016/j.prostaglandins.2017.04.001
Botigelli, R. C., Razza, E. M., Pioltine, E. M., & Nogueira, M. F. G. (2017). New approaches regarding the in vitro maturation of oocytes: manipulating cyclic nucleotides and their partners in crime. JBRA Assisted Reproduction, 21(1), 35-44. doi: 10.5935/1518-0557.20170010 DOI: https://doi.org/10.5935/1518-0557.20170010
Cantanhêde, F. C. (2018). Influência do FSH sobre os aspectos celulares e moleculares na maturação in vitro de complexo’s cumulus-ovócitos de bovinos. Tese de Doutorado, Universidade Federal Rural de Pernambuco, PE, Brasil. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7507
Coelho, L. A. D., Esper, C. R., Alvarez, R. H., Vantine, R., & Almeida, I. L., Jr. (2002). Produção in vitro de embriões bovinos: utilização de diferentes fontes de gonadotrofinas na maturação dos oócitos. Revista Brasileira de Zootecnia, 31(3), 1117-1121. doi: 10.1590/S1516-35982002000500007 DOI: https://doi.org/10.1590/S1516-35982002000500007
De Los Reyes, M., De Lange, J., Miranda, P., Palominos, J., & Barros, C. (2005). Effect of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes. Theriogenology, 64(1), 1-11. doi: 10.1016/j.theriogenology.2004.11.008 DOI: https://doi.org/10.1016/j.theriogenology.2004.11.008
Del Collado, M., Saraiva, N. Z., Lopes, F. L., Gaspar, R. C., Padilha, L. C., Costa, R. R., Rossi, G. F., Vantini, R., & Garcia, J. M. (2015). Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. Reproduction, Fertility and Development, 28(11), 1721-1732. doi: 10.1071/RD15067 DOI: https://doi.org/10.1071/RD15067
Gottardi, F. P., Barretto, L. S. S., Gonçalves, F. S., Perri, S. H. V., & Mingoti, G. Z. (2012). Effects of cumulus cells and cysteamine during bovine oocyte in vitro maturation on meiosis progression and acquisition of developmental competence. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 64(2), 245-252. doi: 10.1590/S0102-09352012000200001 DOI: https://doi.org/10.1590/S0102-09352012000200001
Lee, H. S., Seo, Y. I., Yin, X. J., Cho, S. G., Lee, S. S., Kim, N. H., Cho, S. K., & Kong, I. K. (2007). Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes. Reproduction in Domestic Animals, 42(6), 561-565. doi: 10.1111/j.1439-0531.2006.00818.x DOI: https://doi.org/10.1111/j.1439-0531.2006.00818.x
Mingoti, G. Z., Garcia, J. M., & Rosa-e-Silva, A. A. M. (2002). Steroidogenesis in cumulus cells of bovine cumulus oocyte-complexes matured in vitro with BSA and different concentrations of steroids. Animal Reproduction Science, 69(3-4), 175-186. doi: 10.1016/S0378-4320(01)00187-7 DOI: https://doi.org/10.1016/S0378-4320(01)00187-7
Moussa, M., Shu, J., Zhang, X. H., & Zeng, F. (2015). Maternal control of oocyte quality in cattle “a review”. Animal Reproduction Science, 155(11-27), 11-27. doi: 10.1016/j.anireprosci.2015.01.011 DOI: https://doi.org/10.1016/j.anireprosci.2015.01.011
Ni, H., Sheng, X., Cui, X., Gu, M., Liu, Y., Qi, X., Xing, S., & Guo, Y. (2015). Epidermal growth factor-mediated mitogen-activated protein kinase3/1 pathway is conducive to in vitro maturation of sheep oocytes. Plos One, 10(3), 1-12. doi: 10.1371/journal.pone.0120418 DOI: https://doi.org/10.1371/journal.pone.0120418
Pogrmic-Majkic, K., Samardzija, D., Stojkov-Mimic, N., Vukosavljevic, J., Trninic-Pjevic, A., Kopitovic, V., & Andric, N. A. (2018). Atrazine suppresses FSH-induced steroidogenesis and LH-dependent expression of ovulatory genes through PDE-cAMP signaling pathway in human cumulus granulosa. Molecular and Cellular Endocrinology, 461(1), 79-88. doi: 10.1016/j.mce.2017.08.015 DOI: https://doi.org/10.1016/j.mce.2017.08.015
Rajabi-Toustani, R., Motamedi-Mojdehi, R., Mehr, M. R., & Motamedi-Mojdehi, R. (2013). Effect of Papaver rhoeas L. extract on in vitro maturation of sheep oocytes. Small Ruminant Research, 114(1), 146-151. doi: 10.1016/j.smallrumres.2013.06.010 DOI: https://doi.org/10.1016/j.smallrumres.2013.06.010
Rizos, D., Clemente, M., Bermejo-Alvarez, P., La Fuente, J. de, Lonergan, P., & Gutiérrez-Adan, A. (2008). Consequences of In vitro culture conditions on embryo development and quality. Reproduction in Domestic Animals, 43(4), 44-50. doi: 10.1111/j.1439-0531.2008.01230.x DOI: https://doi.org/10.1111/j.1439-0531.2008.01230.x
Russell, D. L., Gilchrist, R. B., Brown, H. M., & Thompson, J. G. (2016). Bidirectional communication between cumulus cells and the oocyte: old hands and new players? Theriogenology. 86(1), 62-68. doi: 10.1016/j.theriogenology.2016.04.019 DOI: https://doi.org/10.1016/j.theriogenology.2016.04.019
Sha, W., Xu, B. Z., Li, H., Liu, D., Feng, H. L., & Sun, Q. Y. (2010). Effect of gonadotropins on oocyte maturation in vitro: an animal model. Fertiliy and Steriliy, 9(5), 1650-1661. doi: 10.1016/j.fertnstert. 2009.03.003 DOI: https://doi.org/10.1016/j.fertnstert.2009.03.003
Silva, A. A. A., Silva, M. N. P., Figueiredo, L. B. F., Gonçalves, J. D., Silva, M. J. S., Loiola, M. L. G., Bastos, B. D. M., Oliveira, R. A., Ribeiro, L. G. M., Barberino, R. S., Gouveia, B. B., Monte, A. P. O., Nogueira, D. M., Cordeiro, M. F., Matos, M. H. T., & Lopes, E. S., Jr. (2018). Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. Zygote, 26(6), 465-470. doi: 10.1017/S0967199418000485 DOI: https://doi.org/10.1017/S0967199418000485
Souza-Fabjan, J. M. G., Panneau, B., Duffard, N., Locatelli, Y., Figueiredo, J. R., Freitas, V. J. F., & Mermillod, P. (2014). In vitro production of small ruminant embryos: late improvements and further research. Theriogenology, 81(9), 1149-1162. doi: 10.1016/j.theriogenology.2014.02.001 DOI: https://doi.org/10.1016/j.theriogenology.2014.02.001
Sugimura, S., Yamanouchi, T., Palmerini, M. G., Hashiyada, Y., Imai, K., & Gilchrist, R. B. J. (2018). Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle. Journal of Reproduction, 64(3), 233-241. doi: 10.1262/jrd.2018-009 DOI: https://doi.org/10.1262/jrd.2018-009
Tarazona, A. M., Rodriguez, J. I., Restrepo, L. F., & Olivera-Angel, M. (2006). Mitochondrial activity, distribution and segregation in bovine oocytes and in embryos produced in vitro. Reproduction in Domestic Animals, 41(1), 5-11. doi: 10.1111/j.1439-0531.2006.00615.x DOI: https://doi.org/10.1111/j.1439-0531.2006.00615.x
Turathum, B., Gao, E. M., & Chian, R. C. (2021). The function of cumulus cells in oocyte growth and maturation and in subsequent ovulation and fertilization. Cells, 10(9), 2292-2293. doi: 10.3390/cells10092292 DOI: https://doi.org/10.3390/cells10092292
Wei, S. C., Gong, Z. D., Zhao, H. W., Liang, H. Q., Lai, L. J., & Deng, Y. Y. (2016). Equine chorionic gonadotropin influence on sheep oocyte in vitro maturation, apoptosis, and follicle-stimulating hormone receptor and luteinizing hormone receptor expression. Genetics and Molecular Research, 15(4), 1-13. doi: 10.4238/gmr15049162 DOI: https://doi.org/10.4238/gmr15049162
Whitaker, B. D., & Knigh, J. W. (2004). Exogenous γ-glutamyl cycle compounds supplemented to in vitro maturation medium influence in vitro fertilization, culture and embryos parameters of porcine oocytes and embryos. Theriogenology, 62(5), 311-322. doi: 10.1016/j.theriogenology.2003.10.014 DOI: https://doi.org/10.1016/j.theriogenology.2003.10.014
Xiao, X., Zi, X. D., Niu, H. R., Xiong, X. R., Zhong, J. C., Li, J., Wang, L., & Wang, Y. (2014). Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes. Reproductive Biology and Endocrinology, 12(30), 1-7. doi: 10.1186/1477-7827-12-30 DOI: https://doi.org/10.1186/1477-7827-12-30
Zhu, J., Moawad, A. R., Wang, C. Y., Li, H. F., Ren, H. F., & Dai, Y. F. (2018). Advances in in vitro production of sheep embryos. International Journal of Veterinary Science and Medicine, 6(2), S15-S26. doi: 10.1016/j.ijvsm.2018.02.003 DOI: https://doi.org/10.1016/j.ijvsm.2018.02.003
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
