Effect of pigpel extender on the boar semen quality stored at different temperatures
DOI:
https://doi.org/10.5433/1679-0359.2009v30n4p899Keywords:
Semen, Egg yolk, Low density lipoprotein, TemperatureAbstract
This study aim to evaluate the quality of boar semen stored at different temperatures and media on PIGPEL5 extender, using egg yolk as an external membrane protector, and on PIGPEL5Plus, containing low density lipoprotein (LDL) as protector. In the experiment 1, the semen doses were stored in a semen container at 5 and 17 °C and the results were compared with semen stored at 17 °C in BTS extender. In the experiment 2, the doses with the PIGPEL5 and PIGPEL5Plus extenders were stored in a commercial refrigerator and in semen container, both at 5 °C. The use of extenders with extracellular cryoprotectant (PIGPEL5 e PIGPEL5Plus) showed higher (p < 0.05) motility values in comparison to BTS until 48 h. Furthermore, the use of extracellular cryoprotectant was good to maintenance of membrane integrity and normal morphology at 5 oC similarly to 17 oC extenders. There was no difference (p > 0.05) in the motility, membrane integrity and morphology for the substitution of egg yolk (PIGPEL5) for LDL (PIGPEL5Plus). In experiment 2, also there was no difference (p > 0.05) in motility, membrane integrity and morphology between extenders and type of container until 72 h. The conservation of semen on PIGPEL5 extender and commercial refrigerator (5 ºC) allowed patterns of sperm quality according recommended for AI.
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