Genetic characterization of rhizobia isolated from Amazonas soils based on the technique of PCR-RFLP

Authors

  • Aloísio Freitas Chagas Junior UniversidadeFederal de Tocantins
  • Luiz Antonio de Oliveira Instituto Nacional de Pesquisas da Amazônia
  • Arlem Nascimento de Oliveira Universidade Federal do Amazonas

DOI:

https://doi.org/10.5433/1679-0359.2009v30n4p841

Keywords:

Genetic diversity, Microbial ecology, Microbial diversity

Abstract

Few are the details concerning the molecular characterization of native rhizobia isolates from Amazonas soils. The aim of the present study was the genetic characterization of rhizobia isolated from nodules of cowpea using the method PCR-RFLP of the ribosome genes 16S rRNA and the restriction enzymes HinfI, MspI, and MboI. The analysis included 20 native isolates and ten reference strains from different origins. The results indicated a great genetic variability with the formation of eight groups in one dendrogram with a varied level of similarity.

Author Biographies

Aloísio Freitas Chagas Junior, UniversidadeFederal de Tocantins

Professor, Dr. Universidade Federal do Tocantins – Campus Universitário de Gurupi; Cx. Postal: 66, CEP: 77404-970, Gurupi – TO.

Luiz Antonio de Oliveira, Instituto Nacional de Pesquisas da Amazônia

Pesquisador, PhD. Instituto Nacional de Pesquisas da Amazônia, Coordenação de Pesquisas em Ciências Agronômicas; CEP: 69060-000, Manaus - AM.

Arlem Nascimento de Oliveira, Universidade Federal do Amazonas

Professor, Dr. Universidade Federal do Amazonas, Dep. de Ciências Biológicas; CEP: 69077-000, Manaus - AM.

Published

2009-12-20

How to Cite

Chagas Junior, A. F., Oliveira, L. A. de, & Oliveira, A. N. de. (2009). Genetic characterization of rhizobia isolated from Amazonas soils based on the technique of PCR-RFLP. Semina: Ciências Agrárias, 30(4), 841–846. https://doi.org/10.5433/1679-0359.2009v30n4p841

Issue

Section

Communication

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