Can mineral supplementation modify the characteristics of young buffalo ejaculate?
DOI:
https://doi.org/10.5433/1679-0359.2019v40n5Supl1p2271Keywords:
Bubalus bubalis, Mineral mixture, Reproduction, Semen.Abstract
This study aimed to determine the influence of mineral supplementation on the concentration of macroelements and microelements in the seminal plasma and the semen characteristics of young buffaloes. To this end, 60 animals were kept under rotational grazing and were randomly divided into two groups: G-Bov (conventional mineral supplement recommended for bovine cattle; n = 30) and G-Buf (mineral supplement recommended for buffaloes; n = 30). Mineral supplement consumption was calculated from the difference between the amount of supplement offered and the leftover bunk, calculated every 28 days. Eight ejaculates from each animal were collected. The means of the response variables were analyzed using the Kruskal-Wallis test, with a significance level of 5%, and Pearson’s correlation was analyzed between the concentration of minerals in seminal plasma and fresh semen characteristics. Animals in the G-Buf group had lower mineral supplement consumption (G-Bov = 83.18 vs. G-Buf = 77.14 g AU-1 day-1, P < 0.05) and had higher ejaculate volume, sperm motility, sperm vigor, and sperm mass motility than did the animals in the G-Bov group. This research presented the concentration of macroelements and microelements in the seminal plasma of buffaloes receiving different mineral supplements. In addition, the study concluded that the physical and morphological characteristics of the semen of young buffaloes are influenced by the formulation of the mineral supplement, which resulted in better sperm quality in the animals receiving a species-specific supplement. Among the minerals present in buffalo seminal plasma, phosphorus is the element that shows the highest positive correlation with semen characteristics.References
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BARNABE, R. C.; BARNABE, V. H.; OLIVEIRA, C. A.; ARRUDA, R. P.; VALENTIM, R.; ZOGNO, M. A. Biochemical study of semen of buffaloes. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 12., 1992, Hague. Proceeding… Netherlands: ICAR, 1992. v. 3, p. 1523-1524.
BARUSELLI, P. S.; SALES, J. N. S.; SALA, R. V.; VIEIRA, L. M.; SÁ FILHO, M. F. History evolution and perspectives of timed artificial insemination programs in Brazil. Animal Reproduction, Belo Horizonte, v. 9, n. 3, p. 139-152, 2012.
CEROVSKY, J. A. A new staining procedure for boar spermatozoa. Živočišná výroba, Praga, v. 21, n. 5, p. 361-366, 1976. DOI:
DHAMI, A. J.; MOHAN, G.; SAHNI, K. L. Seminal LDH and trace elements in the static versus motile ejaculates of Friesian and Murrah buffalo bulls. International Journal of Animal Sciences, Dover, v. 10, n. 1, p. 151-156, 1995.
DHAMI, A. J.; SHELKE, V. B.; PATEL, K. P.; PARADVA, J. P.; KAVANI, F. S. Trace minerals profile of blood and seminal plasma of breeding bulls. The Indian Journal of Animal Sciences, New Delhi, v. 71, n. 8, p. 761-763, 2001.
EGHBALI, M.; ALAVI-SHOUSHTARI, S. M.; ASRI REZAII, S. Effects of copper and superoxide dismutase content of seminal plasma on buffalo semen characteristics. Pakistan Journal of Biological Sciences, Dubai, v. 11, n. 15, p. 1964-1968, 2008. DOI: 10.3923/pjbs.2008.1964.1968
EGHBALI, M.; ALAVI-SHOUSHTARI, S. M.; ASRI-REZAEI, S.; ANSARI, M. K. Calcium, Magnesium and Total Antioxidant Capacity (TAC) in Seminal Plasma of Water Buffalo (Bubalus bubalis) Bulls and their Relationships with Semen Characteristics. Veterinary Research Forum, Urmia, v. 1, n. 1, p. 12-20, 2010a.
EGHBALI, M.; ALAVI-SHOUSHTARI, S. M.; ASRI-REZAEI, S.; ANSARI, M. K. Effects of the seminal plasma iron and lead content on semen quality of water buffalo (Bubalus bubali) bulls. Veterinary Research Forum, Urmia, v. 1, n. 3, p. 142-148, 2010b.
JAVED, M. T.; KHAN, A.; KAUSAR, R. Influence of age season on body mass, scrotal circumference ande sexaul behaviour os Nili-Ravi buffalo (Bubalus bublais, L.) bulls. Veterinarski Arhiv, Zagreb, v. 68, n. 6, p. 219-229, 1998.
KANWAL, M. R.; REHMAN, N. U.; AHMAD, N.; SAMAD, H. A.; ZIA-UR-REHMAN; AKHTAR, N.; ALI, S. Bulk cations and trace elements in the Nili-Ravi buffalo and crossbred cow bull semen. International Journal of Agriculture and Biology, Faisalabad, v. 2, n. 4, p. 302-305, 2000. DOI: 1560–8530/2000/02–4–302–305
KARESKOSKI, M.; KATILA, T. Components of stallion seminal plasma and the effects of seminal plasma on sperm longevity. Animal Reproduction Science, Amsterdam, v. 107, n. 3-4, p. 249-256, 2008. DOI: 10.1016/j.anireprosci.2008.04.013
KUMAR, M.; PANGAWKAR, G. R.; KUMAR, R. Effect of filtration on seminal plasma electrolytes in buffalo bull. Indian Veterinary Journal, Chennai, v. 68, n. 1, p. 493-494, 1991.
LAND, R. B. Genetics and reproduction. In: AUSTRALIAN, C. R.; SHORT, R. V. Reproduction in mammals. 2th ed. Cambridge: Cambridge University Press, 1985, p. 93-96.
LUZ, P. A. C.; SANTOS, P. R. S.; ANDRIGHETTO, C.; JORGE, A. M.; ASSIS NETO, A. C. The correlation between age, body weight and testicular parameters in murrah buffalo bulls raised in Brazil. Journal of Reproduction and Development, Tokyo, v. 59, n. 1, p. 14-7, 2013. DOI: 10.1262/jrd.2012-021
MARZEC-WRÓBLEWSKA, U.; KAMIŃSKI, P.; ŁAKOTA, P. Influence of chemical elements on mammalian spermatozoa. Folia Biologica, Czech Republic, v. 58, n. 1, p. 7-12, 2012.
MASSÁNYI, P.; TRANDŽÍK, J.; NAD, P.; KORENEKOVÁ, B.; SKALICKÁ, M.; TOMAN R.; LUKÁC, N.; HALO, M.; STRAPÁK, P. Concentration of copper, iron, zinc, cadmium, lead and nickel in bull and ram semen and relation to the occurrence of pathological spermatozoa. Journal of Environmental Science and Health Part A, Fairfax, v. 39, n. 11-12, p. 3005-3014, 2004. DOI: 10.1081/LESA-200034832
MASSÁNYI, P.; TRANDŽÍK, J.; NAD, P.; KORENOKOVÁ, B.; SKALICKÁ, M.; TOMAN, R.; LUKÁC, N.; STRAPÁK, P.; HALO, M.; TURCAN, J. Concentration of copper, iron, zinc, cadmium, lead and nickel in boar semen and relation to the spermatozoa quality. Journal of Environmental Science and Health, Part A, Fairfax, v. 38, n. 11, p. 2643-2651, 2003. DOI: https://doi.org/10.1081/ESE-120024453
MASSÁNYI, P.; WEIS, J.; LUKAC, N.; TRANDŽÍK, J.; BYSTRICKA, J. Cadmium, zinc, copper, sodium and potassium concentrations in rooster and turkey semen and their correlation. Journal of Environmental Science and Health, Part A, Fairfax, v. 43, n. 5, p. 563-565, 2008. DOI: 10.1080/10934520701796655
MILLES, S. P. H.; WILKINSON, N. S.; MCDOWELL, L.R. Analysis of minerals for animal nutrition research. 3th ed. Flórida: UFL, 2001. 117 p.
HOPPER, R. M. Breeding Soundness Examination in the Bull. In: Bovine Reproduction, HOPPER, R. M. (Ed.), Mississippi: John Wiley & Sons, Inc., 2014, cap. 6, p. 58-63. DOI: 10.1002/9781118833971.ch6
PARVINEN, M. Regulation of seminiferous epithelium in bulls. Endocrine Reviews., Washington, v. 3, n. 4, p. 404- 417, 1982. DOI: 10.1210/edrv-3-4-404
PEIXOTO, G. C.; SILVA, M. A.; CASTELO, T. S.; SILVA, A. M.; BEZERRA, J. A.; SOUZA, A. L.; OLIVEIRA, M. F.; SILVA, A. R. Individual variation related to testicular biometry and semen characteristics in collared peccaries (Tayassu Tajacu Linnaeus, 1758). Animal Reproduction Science, Amsterdam, v. 134, n. 3-4, p. 191-196, 2012. DOI: 10.1016/j.anireprosci.2012.08.026
ROYCHOUDHURY, S.; SLIVKOVÁ, J.; BULLA, J.; MASSÁNYI, P. Copper administration alerts fine parameters of spermatozoa motility in vitro. Folia Veterinaria, Czech Republic, v. 52, n. 2, p. 64-68, 2008.
SÁ FILHO, M. F.; BALDRIGHI, J. M.; SALES, J. N. S.; CREPALDI, G. A.; CARVALHO, J. B. P.; BÓ, G. A.; BARUSELLI, P. S. Induction of ovarian follicular wave emergence and ovulation in progestin-based time artificial insemination protocols for Bos indicus cattle. Animal Reproduction Science, Amsterdam, v. 129, n. 3 - 4, p. 132-139, 2011. DOI: 10.1016/j.anireprosci.2011.12.005
SANSONE, G.; NASTRI, M. J. F.; FABBROCINI, A. Storage of buffalo (Bubalus bubalis) semen. Animal Reproduction Science, Amsterdam, v. 62, n. 1-3, p. 55-76, 2000. DOI: 10.1016/S0378-4320(00)00154-8
SHELKE, V. B.; DHAMI, A. J. Comparative appraisal of blood and seminal plasma biochemistry of Gir and Jafarabadi breeding bulls. Indian Veterinary Journal, Chennai, v. 79, n. 4, p. 349-353, 2002a.
SHELKE, V. B.; DHAMI, A. J. Interrelationships of seminal plasma biochemical, enzymatic and macro-micro mineral profiles with physical attributes and freezability of semen in Jafarabadi bulls. Indian Journal of Animal Sciences, New Delhi, v. 72, n. 5, p. 386-390, 2002b.
SILVA, D. J. Análises de alimentos: métodos químicos e biológicos. 2. ed. Viçosa, MG: UFV, 1998. 165 p.
SØRENSEN, M. B.; BERGDAHL, I. A.; HJØLLUND, N. H. I.; BONDE, J. P. E.; STOLTENBERG, M.; ERNST, E. Zinc, magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility. Molecular Human Reproduction, Oxford, v. 5, n. 4, p. 331-337, 1999.
TVRDÁ, E.; KŇAŽICKÁ, Z.; LUKÁČOVÁ, J.; SCHNEIDGENOVÁ, M.; GOC, Z.; GREŃ, A.; SZABÓ, C.; MASSÁNYI, P.; LUKÁČ, N. The impact of lead and cadmium on selected motility, prooxidant and antioxidant parameters of bovine seminal plasma and spermatozoa. Journal of Environmental Science and Health, New Jersey, v. 48, n. 10, p. 1292-1300, 2013. DOI: https://doi.org/10.1080/10934529.2013.777243
UNANIAN, M. M.; SILVA, A. E. D. F.; DODE, M. A. N. Valores de zinco no plasma seminal de soro sanguíneo de bovinos púberes da raça Nelore relacionados às características espermáticas e desenvolvimento reprodutivo. Pesquisa Agropecuária Brasileira, Brasília, v. 26, n. 4, p. 597-604, 1991.
VALE, W. G. Buffalo production, in the Amazon valley. In: INTERNATIONAL SYMPOSIUM ON BUFFALO PRODUCTS, 1994, Paestum. Proceedings... Paestum: Wageningen press, (EAAP Publication, 82), 1994b. p. 99-108.
VALE, W. G. Collection, processing and deep freezing of buffalo semen. Buffalo Journal, Bangkok, v. 2, suppl. 2, p. 65-81, 1994a.
VALE, W. G.; OHASHI, O. M.; RIBEIRO, H. F. L.; SOUSA, J. S. Semen freezing and artificial insemination in water buffalo in the Amazon valley. Buffalo Journal, Bangkok, v. 7, n. 2, p. 137-144, 1991.
VIANA, R. B.; BRAGANÇA, G. M.; CARDOSO, E. C.; MONTEIRO, B. M.; ARAÚJO, C. V.; PAZ, G. C.; SOUZA, D. C. Preparo de amostras de plasma seminal de búfalos para determinação de minerais. Revista de Ciências Agrárias, Belém, v. 54, n. 3, p. 208-213, 2011. DOI: 10.4322/rca.2012.016
ANDRABI, S. M. H. Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermartozoa. Reproduction in Domestic Animals, Berlin, v. 44, n.3, p. 552-569, 2009. DOI: 10.1111/j.1439-0531.2008.01240.x
ATIG, F.; RAFFA, M.; BEN-ALI, H.; KERKENI, A.; SAAD, A.; AJINA, M. Impact of seminal trace element and glutathione levels on semen quality of Tunisian infertile men. BMC Urology, London, v. 12, n. 6, p. 2-8, 2012. DOI: 10.1186/1471-2490-12-6
BARNABE, R. C.; BARNABE, V. H.; OLIVEIRA, C. A.; ARRUDA, R. P.; VALENTIM, R.; ZOGNO, M. A. Biochemical study of semen of buffaloes. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 12., 1992, Hague. Proceeding… Netherlands: ICAR, 1992. v. 3, p. 1523-1524.
BARUSELLI, P. S.; SALES, J. N. S.; SALA, R. V.; VIEIRA, L. M.; SÁ FILHO, M. F. History evolution and perspectives of timed artificial insemination programs in Brazil. Animal Reproduction, Belo Horizonte, v. 9, n. 3, p. 139-152, 2012.
CEROVSKY, J. A. A new staining procedure for boar spermatozoa. Živočišná výroba, Praga, v. 21, n. 5, p. 361-366, 1976. DOI:
DHAMI, A. J.; MOHAN, G.; SAHNI, K. L. Seminal LDH and trace elements in the static versus motile ejaculates of Friesian and Murrah buffalo bulls. International Journal of Animal Sciences, Dover, v. 10, n. 1, p. 151-156, 1995.
DHAMI, A. J.; SHELKE, V. B.; PATEL, K. P.; PARADVA, J. P.; KAVANI, F. S. Trace minerals profile of blood and seminal plasma of breeding bulls. The Indian Journal of Animal Sciences, New Delhi, v. 71, n. 8, p. 761-763, 2001.
EGHBALI, M.; ALAVI-SHOUSHTARI, S. M.; ASRI REZAII, S. Effects of copper and superoxide dismutase content of seminal plasma on buffalo semen characteristics. Pakistan Journal of Biological Sciences, Dubai, v. 11, n. 15, p. 1964-1968, 2008. DOI: 10.3923/pjbs.2008.1964.1968
EGHBALI, M.; ALAVI-SHOUSHTARI, S. M.; ASRI-REZAEI, S.; ANSARI, M. K. Calcium, Magnesium and Total Antioxidant Capacity (TAC) in Seminal Plasma of Water Buffalo (Bubalus bubalis) Bulls and their Relationships with Semen Characteristics. Veterinary Research Forum, Urmia, v. 1, n. 1, p. 12-20, 2010a.
EGHBALI, M.; ALAVI-SHOUSHTARI, S. M.; ASRI-REZAEI, S.; ANSARI, M. K. Effects of the seminal plasma iron and lead content on semen quality of water buffalo (Bubalus bubali) bulls. Veterinary Research Forum, Urmia, v. 1, n. 3, p. 142-148, 2010b.
JAVED, M. T.; KHAN, A.; KAUSAR, R. Influence of age season on body mass, scrotal circumference ande sexaul behaviour os Nili-Ravi buffalo (Bubalus bublais, L.) bulls. Veterinarski Arhiv, Zagreb, v. 68, n. 6, p. 219-229, 1998.
KANWAL, M. R.; REHMAN, N. U.; AHMAD, N.; SAMAD, H. A.; ZIA-UR-REHMAN; AKHTAR, N.; ALI, S. Bulk cations and trace elements in the Nili-Ravi buffalo and crossbred cow bull semen. International Journal of Agriculture and Biology, Faisalabad, v. 2, n. 4, p. 302-305, 2000. DOI: 1560–8530/2000/02–4–302–305
KARESKOSKI, M.; KATILA, T. Components of stallion seminal plasma and the effects of seminal plasma on sperm longevity. Animal Reproduction Science, Amsterdam, v. 107, n. 3-4, p. 249-256, 2008. DOI: 10.1016/j.anireprosci.2008.04.013
KUMAR, M.; PANGAWKAR, G. R.; KUMAR, R. Effect of filtration on seminal plasma electrolytes in buffalo bull. Indian Veterinary Journal, Chennai, v. 68, n. 1, p. 493-494, 1991.
LAND, R. B. Genetics and reproduction. In: AUSTRALIAN, C. R.; SHORT, R. V. Reproduction in mammals. 2th ed. Cambridge: Cambridge University Press, 1985, p. 93-96.
LUZ, P. A. C.; SANTOS, P. R. S.; ANDRIGHETTO, C.; JORGE, A. M.; ASSIS NETO, A. C. The correlation between age, body weight and testicular parameters in murrah buffalo bulls raised in Brazil. Journal of Reproduction and Development, Tokyo, v. 59, n. 1, p. 14-7, 2013. DOI: 10.1262/jrd.2012-021
MARZEC-WRÓBLEWSKA, U.; KAMIŃSKI, P.; ŁAKOTA, P. Influence of chemical elements on mammalian spermatozoa. Folia Biologica, Czech Republic, v. 58, n. 1, p. 7-12, 2012.
MASSÁNYI, P.; TRANDŽÍK, J.; NAD, P.; KORENEKOVÁ, B.; SKALICKÁ, M.; TOMAN R.; LUKÁC, N.; HALO, M.; STRAPÁK, P. Concentration of copper, iron, zinc, cadmium, lead and nickel in bull and ram semen and relation to the occurrence of pathological spermatozoa. Journal of Environmental Science and Health Part A, Fairfax, v. 39, n. 11-12, p. 3005-3014, 2004. DOI: 10.1081/LESA-200034832
MASSÁNYI, P.; TRANDŽÍK, J.; NAD, P.; KORENOKOVÁ, B.; SKALICKÁ, M.; TOMAN, R.; LUKÁC, N.; STRAPÁK, P.; HALO, M.; TURCAN, J. Concentration of copper, iron, zinc, cadmium, lead and nickel in boar semen and relation to the spermatozoa quality. Journal of Environmental Science and Health, Part A, Fairfax, v. 38, n. 11, p. 2643-2651, 2003. DOI: https://doi.org/10.1081/ESE-120024453
MASSÁNYI, P.; WEIS, J.; LUKAC, N.; TRANDŽÍK, J.; BYSTRICKA, J. Cadmium, zinc, copper, sodium and potassium concentrations in rooster and turkey semen and their correlation. Journal of Environmental Science and Health, Part A, Fairfax, v. 43, n. 5, p. 563-565, 2008. DOI: 10.1080/10934520701796655
MILLES, S. P. H.; WILKINSON, N. S.; MCDOWELL, L.R. Analysis of minerals for animal nutrition research. 3th ed. Flórida: UFL, 2001. 117 p.
HOPPER, R. M. Breeding Soundness Examination in the Bull. In: Bovine Reproduction, HOPPER, R. M. (Ed.), Mississippi: John Wiley & Sons, Inc., 2014, cap. 6, p. 58-63. DOI: 10.1002/9781118833971.ch6
PARVINEN, M. Regulation of seminiferous epithelium in bulls. Endocrine Reviews., Washington, v. 3, n. 4, p. 404- 417, 1982. DOI: 10.1210/edrv-3-4-404
PEIXOTO, G. C.; SILVA, M. A.; CASTELO, T. S.; SILVA, A. M.; BEZERRA, J. A.; SOUZA, A. L.; OLIVEIRA, M. F.; SILVA, A. R. Individual variation related to testicular biometry and semen characteristics in collared peccaries (Tayassu Tajacu Linnaeus, 1758). Animal Reproduction Science, Amsterdam, v. 134, n. 3-4, p. 191-196, 2012. DOI: 10.1016/j.anireprosci.2012.08.026
ROYCHOUDHURY, S.; SLIVKOVÁ, J.; BULLA, J.; MASSÁNYI, P. Copper administration alerts fine parameters of spermatozoa motility in vitro. Folia Veterinaria, Czech Republic, v. 52, n. 2, p. 64-68, 2008.
SÁ FILHO, M. F.; BALDRIGHI, J. M.; SALES, J. N. S.; CREPALDI, G. A.; CARVALHO, J. B. P.; BÓ, G. A.; BARUSELLI, P. S. Induction of ovarian follicular wave emergence and ovulation in progestin-based time artificial insemination protocols for Bos indicus cattle. Animal Reproduction Science, Amsterdam, v. 129, n. 3 - 4, p. 132-139, 2011. DOI: 10.1016/j.anireprosci.2011.12.005
SANSONE, G.; NASTRI, M. J. F.; FABBROCINI, A. Storage of buffalo (Bubalus bubalis) semen. Animal Reproduction Science, Amsterdam, v. 62, n. 1-3, p. 55-76, 2000. DOI: 10.1016/S0378-4320(00)00154-8
SHELKE, V. B.; DHAMI, A. J. Comparative appraisal of blood and seminal plasma biochemistry of Gir and Jafarabadi breeding bulls. Indian Veterinary Journal, Chennai, v. 79, n. 4, p. 349-353, 2002a.
SHELKE, V. B.; DHAMI, A. J. Interrelationships of seminal plasma biochemical, enzymatic and macro-micro mineral profiles with physical attributes and freezability of semen in Jafarabadi bulls. Indian Journal of Animal Sciences, New Delhi, v. 72, n. 5, p. 386-390, 2002b.
SILVA, D. J. Análises de alimentos: métodos químicos e biológicos. 2. ed. Viçosa, MG: UFV, 1998. 165 p.
SØRENSEN, M. B.; BERGDAHL, I. A.; HJØLLUND, N. H. I.; BONDE, J. P. E.; STOLTENBERG, M.; ERNST, E. Zinc, magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility. Molecular Human Reproduction, Oxford, v. 5, n. 4, p. 331-337, 1999.
TVRDÁ, E.; KŇAŽICKÁ, Z.; LUKÁČOVÁ, J.; SCHNEIDGENOVÁ, M.; GOC, Z.; GREŃ, A.; SZABÓ, C.; MASSÁNYI, P.; LUKÁČ, N. The impact of lead and cadmium on selected motility, prooxidant and antioxidant parameters of bovine seminal plasma and spermatozoa. Journal of Environmental Science and Health, New Jersey, v. 48, n. 10, p. 1292-1300, 2013. DOI: https://doi.org/10.1080/10934529.2013.777243
UNANIAN, M. M.; SILVA, A. E. D. F.; DODE, M. A. N. Valores de zinco no plasma seminal de soro sanguíneo de bovinos púberes da raça Nelore relacionados às características espermáticas e desenvolvimento reprodutivo. Pesquisa Agropecuária Brasileira, Brasília, v. 26, n. 4, p. 597-604, 1991.
VALE, W. G. Buffalo production, in the Amazon valley. In: INTERNATIONAL SYMPOSIUM ON BUFFALO PRODUCTS, 1994, Paestum. Proceedings... Paestum: Wageningen press, (EAAP Publication, 82), 1994b. p. 99-108.
VALE, W. G. Collection, processing and deep freezing of buffalo semen. Buffalo Journal, Bangkok, v. 2, suppl. 2, p. 65-81, 1994a.
VALE, W. G.; OHASHI, O. M.; RIBEIRO, H. F. L.; SOUSA, J. S. Semen freezing and artificial insemination in water buffalo in the Amazon valley. Buffalo Journal, Bangkok, v. 7, n. 2, p. 137-144, 1991.
VIANA, R. B.; BRAGANÇA, G. M.; CARDOSO, E. C.; MONTEIRO, B. M.; ARAÚJO, C. V.; PAZ, G. C.; SOUZA, D. C. Preparo de amostras de plasma seminal de búfalos para determinação de minerais. Revista de Ciências Agrárias, Belém, v. 54, n. 3, p. 208-213, 2011. DOI: 10.4322/rca.2012.016
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2019-08-07
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Viana, R. B., Monteiro, B. M., Cardoso, E. da C., Ribeiro Filho, J. D., Albuquerque, R. dos S., Melo, P. M. A. de, & Baruselli, P. S. (2019). Can mineral supplementation modify the characteristics of young buffalo ejaculate?. Semina: Ciências Agrárias, 40(5Supl1), 2271–2286. https://doi.org/10.5433/1679-0359.2019v40n5Supl1p2271
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