Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis

Authors

  • Jéssica Fontes Veloso Universidade Estadual de Santa Cruz
  • Leonardo Sauer Médico Veterinário Autônomo
  • Arianne Pontes Oriá Universidade Federal da Bahia
  • Deusdete Conceição Gomes Junior Universidade Federal do Oeste da Bahia
  • Ana Cláudia Santos Raposo Universidade Federal da Bahia
  • Camila Fernanda Oliveira Andrade Médico Veterinário Autônomo
  • Thais Nascimento de Andrade Oliveira Universidade Estadual de Santa Cruz
  • Renata Santiago Alberto Carlos Universidade Estadual de Santa Cruz

DOI:

https://doi.org/10.5433/1679-0359.2018v39n3p1049

Keywords:

Eye, Glaucoma, Infectious Uveitis, Nested PCR, Rickettsia.

Abstract

Canine monocytic ehrlichiosis (CME) is an infectious disease caused by a gram-negative bacterium Ehrlichia canis that has a high global prevalence that leads to high rates of morbidity and mortality in dogs. Among the clinical changes, ophthalmic diseases can lead to permanent blindness and it can be an important clinical sign. The objective of this study was to perform nested polymerase chain reaction (PCR) to diagnose E. canis infection in dogs with bilateral uveitis from the Veterinary Hospital of the Santa Cruz State University. Blood samples were collected and DNA for the molecular diagnosis was extracted from 66 adult dogs of both genders and mixed breeds diagnosed with bilateral uveitis. Thirty-five (53%) dogs showed positive results and presented with iridocyclitis, posterior uveitis, panuveitis, or uveitis with secondary glaucoma. This study demonstrates that nested PCR is an important tool for the differential diagnosis of dogs with bilateral uveitis, as it provides evidence of the infectious agent in the animal.

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Author Biographies

Jéssica Fontes Veloso, Universidade Estadual de Santa Cruz

Discente de Doutorado, Universidade Estadual de Santa Cruz, UESC, Ilhéus, BA, Brasil.

Leonardo Sauer, Médico Veterinário Autônomo

Médico Veterinário Autônomo, Bahia, BA, Brasil.

Arianne Pontes Oriá, Universidade Federal da Bahia

Profª, Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, UFBA, Salvador, BA, Brasil.

Deusdete Conceição Gomes Junior, Universidade Federal do Oeste da Bahia

Profª, Universidade Federal do Oeste da Bahia, UFOB, Barra, BA, Brasil.

Ana Cláudia Santos Raposo, Universidade Federal da Bahia

Discente de Doutorado, UFBA, Salvador, BA, Brasil.

Camila Fernanda Oliveira Andrade, Médico Veterinário Autônomo

Médico Veterinário Autônomo, Bahia, BA, Brasil.

Thais Nascimento de Andrade Oliveira, Universidade Estadual de Santa Cruz

Discente de Doutorado, Universidade Estadual de Santa Cruz, UESC, Ilhéus, BA, Brasil.

Renata Santiago Alberto Carlos, Universidade Estadual de Santa Cruz

Profª, Universidade Estadual de Santa Cruz, UESC, Bahia, BA, Brasil.

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Published

2018-05-04

How to Cite

Veloso, J. F., Sauer, L., Oriá, A. P., Gomes Junior, D. C., Raposo, A. C. S., Andrade, C. F. O., … Carlos, R. S. A. (2018). Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis. Semina: Ciências Agrárias, 39(3), 1049–1056. https://doi.org/10.5433/1679-0359.2018v39n3p1049

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