Proliferative activity of oocytes in multi-oocyte follicles of bovine ovary
DOI:
https://doi.org/10.5433/1679-0359.2017v38n6p3591Keywords:
PCNA, Cattle, Folliculogenesis, Multiple oocytes.Abstract
We characterized the proliferative activity of multi-oocyte follicles with anti-nuclear antigen of proliferating cells (PCNA). Ovaries (n = 12) from heifers were processed for histology. From 789 multi-oocytes follicles observed, only 11 were considered appropriated for immunostaining, since they presented all nuclei of the oocytes clearly visible. All multi-oocyte follicles were positive for PCNA, but some oocytes showed no proliferative activity. We conclude that oocytes in multi-oocyte follicles seem to be in different stages of the cell cycle.Downloads
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