Humoral immune response of horses experimentally infected with Trypanosoma evansi

Authors

  • Luiz Carlos Marques Universidade Estadual Paulista
  • Rosângela Zacarias Machado Universidade Estadual Paulista
  • Antônio Carlos Alessi Universidade Estadual Paulista
  • Lúcia Padilha Cury Thomaz de Aquino Universidade Estadual Paulista

DOI:

https://doi.org/10.5433/1679-0359.2001v22n2p131

Keywords:

Trypanosoma evansi, Trypanosomiasis, Horses, Immune response.

Abstract

Six adult horses were experimentally infected with Trypanosoma evansi (106 parasites). Three other adult horses served as negative control. Serum samples of the experimentally infected horses with T. evansi and non-infected controls horses were obtained before inoculation, and daily thereafter until 14 days post infection (DPI). After that time the serum samples were obtained weekly. Sera of the infected and non-infected control horses was tested by indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against T. evansi. Both ELISA and IFAT detected trypanosomal antibodies shortly after infection and showed progressive increases in antibodies levels during early stages of infection. The responses started on the eighth and eleventh DPI. Maximum IFAT and ELISA values were reached after four weeks of infection and were maintained at this level until the end of the period of study.

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Author Biographies

Luiz Carlos Marques, Universidade Estadual Paulista

Professor da Faculdade de Ciências Agrárias e Veterinárias, UNESP/Jaboticabal. Via de Acesso Prof. Paulo Donato Castellane s/n.144884-900 Jaboticabal, SP.

Rosângela Zacarias Machado, Universidade Estadual Paulista

Professor da Faculdade de Ciências Agrárias e Veterinárias, UNESP/Jaboticabal. Via de Acesso Prof. Paulo Donato Castellane s/n.144884-900 Jaboticabal, SP.

Antônio Carlos Alessi, Universidade Estadual Paulista

Professor da Faculdade de Ciências Agrárias e Veterinárias, UNESP/Jaboticabal. Via de Acesso Prof. Paulo Donato Castellane s/n.144884-900 Jaboticabal, SP.

Lúcia Padilha Cury Thomaz de Aquino, Universidade Estadual Paulista

Doutoranda do curso de pós-graduação em Medicina Veterinária (Patologia Animal), UNESP/Jaboticabal.

Published

2001-02-28

How to Cite

Marques, L. C., Machado, R. Z., Alessi, A. C., & Aquino, L. P. C. T. de. (2001). Humoral immune response of horses experimentally infected with Trypanosoma evansi. Semina: Ciências Agrárias, 22(2), 131–133. https://doi.org/10.5433/1679-0359.2001v22n2p131

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