Micropropagation of orchid hybrids in knudson culture medium with addiction of vitamins of ms culture medium, benzilaminopurine and activated charcoal

Abstract

The genera Cattleya and Brassavola, naturally occurring in Brazil, are widely used in hybridization to obtain better shape of the lip in the hybrids. This demand creates the need to develop more effective propagation to meet the market and contributing to avoid the extinction of these plants. This study aimed to test BAP concentrations, activated charcoal, culture medium Knudson and culture medium MS’s vitamins in subculture of Brassocattleya Pastoral x Laeliocattleya Amber Glow orchids plants. There were used seedlings from in vitro germination, with 1-1.5 cm length, undergoing standardization in the culture medium Knudson for three months. The experiment were performed using concentrations of culture medium Knudson (0%, 50%, 100%, 150% and 200%), combined with concentrations of culture medium MS’s vitamins (0%, 50%, 100% and 200%) and BAP concentrations (0; 1.0; 2.0 and 4.0 mg L-1) versus activated charcoal (0, 200, 400 and 600 mg L-1), incorporated in a culture medium Knudson. In each flask of 250 mL containing approximately 50 mL of culture medium, four seedlings were placed under aseptic conditions. It was concluded that can use 129% of Knudson, supplemented with 137.5 mg L-1 activated charcoal, 104% of vitamin MS medium without addition of BAP as the best composition for culture medium of hybrid orchid in vitro propagation. The Knudson culture medium does not promote increase in the root system.