Identification of a species-specific protein of toxigenic Fusarium verticillioides

Aline Myuki Omori; Elisabete Yurie Sataque Ono; Igor Massahiro de Souza Suguiura; Amanda Kaori Sugahara; Elisa Yoko Hirooka; Mario Augusto Ono

doi:10.5433/1679-0359.2025v46n5p1347

Authors

Aline Myuki Omori Universidade Estadual de Londrina https://orcid.org/0000-0002-3987-4595
Elisabete Yurie Sataque Ono Universidade Estadual de Londrina https://orcid.org/0000-0003-4461-4525
Igor Massahiro de Souza Suguiura Universidade Estadual de Londrina https://orcid.org/0000-0001-7160-319X
Amanda Kaori Sugahara Universidade Estadual de Londrina https://orcid.org/0009-0004-4244-6980
Elisa Yoko Hirooka Universidade Estadual de Londrina https://orcid.org/0000-0003-0481-1188
Mario Augusto Ono Universidade Estadual de Londrina https://orcid.org/0000-0002-8691-5118

DOI:

https://doi.org/10.5433/1679-0359.2025v46n5p1347

Keywords:

Corn, Food-borne fungi, Fumonisin, Immunodetection, Mycotoxin, Toxigenic fungi.

Abstract

Fusarium verticillioides is a primary corn pathogen and the main producer of fumonisins, a group of toxic secondary metabolites that can cause human and animal health hazards. In humans, epidemiological studies have associated fumonisins with esophageal and liver cancer and have been classified by the International Agency for Research on Cancer – IARC in group 2B (possibly carcinogenic to humans). The development of a rapid, sensitive and specific method to detect this fungus is essential and immunological detection is a promising approach. The discovery, identification and characterization of species-specific antigens are important for the development of a specific immunological technique. Therefore, the objective of this study was to identify the 67 kDa protein (p67) of F. verticillioides exoantigen which shows high potential to be a species-specific antigen. p67 was identified by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and by in-gel analysis of the enzymatic activity (amylase and protease) using polyacrylamide gel co-polymerized with starch, gelatin, and bovine serum albumin. The analysis of in-gel trypsin digested p67 by mass spectrometry revealed two possible proteins: the protein related to aminopeptidase Y precursor of F. fujikuroi and the putative glucoamylase GMY2 of F. verticillioides. In order to confirm which of these proteins the p67 is, both the enzymatic activities were evaluated and showed that this protein presents an amylase activity indicating that p67 is the putative glucoamylase GMY2 of F. verticillioides.

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Author Biographies

Aline Myuki Omori , Universidade Estadual de Londrina

Dra., Experimental Pathology, Department of Immunology, Parasitology and General Pathology, Center of Biological Sciences, Universidade Estadual de Londrina, UEL, Londrina, PR, Brazil.

Elisabete Yurie Sataque Ono, Universidade Estadual de Londrina

Profa. Dra., Department of Biochemistry and Biotechnology, Center of Exact Sciences, UEL, Londrina, PR, Brazil.

Igor Massahiro de Souza Suguiura , Universidade Estadual de Londrina

Dr., Experimental Pathology, Department of Immunology, Parasitology and General Pathology, Center of Biological Sciences, Universidade Estadual de Londrina, UEL, Londrina, PR, Brazil.

Amanda Kaori Sugahara, Universidade Estadual de Londrina

Ms Student of Biotechnology Program, Department of Biochemistry and Biotechnology, Center of Exact Sciences, UEL, Londrina, PR, Brazil.

Elisa Yoko Hirooka, Universidade Estadual de Londrina

Profa.Dra., Department of Food Science and Technology, Center of Agricultural Sciences, UEL, Londrina, PR, Brazil.

Mario Augusto Ono, Universidade Estadual de Londrina

Prof. Dr., Department of Immunology, Parasitology and General Pathology, Center of Biological Sciences, State University of Londrina, UEL, Londrina, PR, Brazil.

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