Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters

Abstract

This study aimed to evaluate the effect of freezing (- 20 ºC) and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava - PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero), after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST), urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC) was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times.