Comparative analysis of the hormone production and gene expression profiles in ovine uterus tissue during oestrus cycle synchronized using medroxyprogesterone acetate plus eCG and prostaglandin analogue
DOI:
https://doi.org/10.5433/1679-0359.2021v42n6p3321Keywords:
Oestrus cycle, Progesterone receptor, Estrogen receptor-alfa, IFNAR1, Sheep.Abstract
The combination of medroxyprogesterone acetate (MPA) and gonadotropin chorionic (eCG) has been widely used to synchronize oestrus cycle in sheep, but their effects on the gene expression in uterine tissue are yet to be elucidated. To evaluate the effect of MPA + eCG or prostaglandin analogue (PA) treatments on the rate of oestrus cycle synchronization, as well as further hormone production and gene expression profiles in uterine tissue, 14 Santa Inês ewes were randomly selected. The MPA + eCG group (n = 7) received intravaginal insertion of MPA-impregnated sponges for 14 days and was administered 350 IU eCG on the day of sponge withdrawal. The PA group (n = 7) was administered two doses of 100 ug of PA separated by 12 days. The ewes were assessed for the rate of oestrus cycle synchronization and the serum concentrations of progesterone (P4) and estradiol (E2). Additionally, the expression of estrogen receptor (ERalfa), progesterone receptor (P4R), and immunolocalization of interferon receptor (IFNAR1) in the uterine tissue samples collected 15 th day post-mating were examined. The rate of oestrus cycle synchronization was 100% (n=7/7) and 57.14% (n=4/7) in the MPA + eCG and PA groups, respectively. Moreover, the MPA + eCG group exhibited higher serum concentration of P4 than the PA group (p smaller 0.05). However, the E2 serum concentration did not differ between the two groups (p larger 0.05). The relative expression of P4R and ERalfa mRNA analyzed using real-time PCR and immunodetection of IFNAR1 were similar between the two groups tested (p larger 0.05). Conclusively, MPA + eCG treatment improved the rate of oestrus cycle synchronization and endogenous P4 production; however, it did not affect the expression of sex steroid receptors and IFNAR1 in uterine ovine tissue.Metrics
Metrics Loading ...
References
Abecia, J. A., Forcada, F., & González-Bulnes, A. (2012). Hormonal control of reproduction in small ruminants. Animal Reproduction Science, 130(3-4), 173-179. doi: 10.1016/j.anireprosci.2012.01.011
Anjos, D. S. dos, Vital, A. F., Lainetti, P. F., Leis, A. F., F., Dalmolin, F., Elias, F., Fonseca-Alves, C. E. (2019). Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis. Veterinary Science, 6(1), 1-12. doi: 10.3390/vetsci6010003
Araujo, R. R., Ginther, O. J., Ferreira, J. C., Palhão, M. M., Beg, M. A., & Wiltbank, M. C. (2009). Role of follicular estradiol-17beta in timing of luteolysis in heifers. Biology of Reproduction, 81(2), 426-437. doi: 10.1095/biolreprod.108.073825
Arsoy, D., & Sağmanlıgil, V. (2018). Reproductive cycles in white Karaman ewes: comparison of ovarian hormone secretion and reproductive behavior in non-pregnant and pregnant ewes in semi-intensive conditions. Acta Scientiarum. Animal Sciences, 40(1), 1-7. doi: 10.4025/actascianimsci.v40i1.39908
Bartlewski, P. M., Baby, T. E., & Giffin, J. L. (2011). Reproductive cycles in sheep. Animal Reproduction Science, 124(3-4), 259-268. doi: 10.1016/j.anireprosci.2011.02.024
Bartlewski, P. M., Beard, A. P., Cook, S. J., Chandolia, R. K, Honaramooz, A., & Rawlings, N. C. (1999). Ovarian antral follicular dynamics and their relationships with endocrine variables throughout the oestrous cycle in breeds of sheep differing in prolificacy. Journal of Reproduction and Fertility, 115(1), 111-124. doi: 10.1530/jrf.0.1150111
Bazer, F. W. (2013). Pregnancy recognition signaling mechanisms in ruminants and pigs. Journal of Animal Science and Biotechnology, 4(1), 1-10. doi: 10.1186/2049-1891-4-23
Bazer, F. W., Spencer, T. E., & Ott, T. L. (1997). Interferon tau: a novel pregnancy recognition signal. American Journal of Reproductive Immunology, 37(6), 412-20. doi: 10.1111/j.1600-0897.1997.tb0025 3.x
Bragança, G. M., Batista, R. I. T. P., Souza-Fabjan, J. M. G., Alfradique, V. A. P., Arashiro, E. K. N., Pinto, P. H. N.,… Brandão, F. Z. (2021). Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep. Domestic Animal Endocrinology, 74, 106518. doi: 10.1016/j.domaniend.2020.106518
Brooks, K., & Spencer, T. (2015). Biological roles of interferon tau (IFNT) and type I IFN receptors in elongation of the ovine conceptus. Biology of Reproduction, 92(2), 1-10. doi: 1095/biolreprod.114.1241 56
Cosentino, I. O., Balaro, M. F. A., Arashiro, E. K. N., Santos, J. D. R., Carvalho, A. B. S., Clariget, R. P.,… Brandão, F. Z. (2019). Hormonal protocols for early resynchronization of ovulation in ewes: The use of progestagens, eCG, and inclusion of early pregnancy diagnosis with color Doppler ultrasound. Theriogenology, 133(15), 113-118. doi: 10.1016/j.theriogenology.2019.04.033
Gao, X., Zhang Q., Zhu, M., Li, X., Guo, Y., Pang, J.,… Wang, F. (2019). Effects of l-arginine on endometrial estrogen receptor α/β and progesterone receptor expression in nutrient-restricted sheep. Theriogenology, 138(15), 137-144. doi: 10.1016/j.theriogenology.2019.07.018
García-Palencia, P., Sánchez, M. A., Nieto, A., Vilar, M. P., González, M., Veiga-Lopez, A.,… Flores, J. M. (2007). Sex steroid receptor expression in the oviduct and uterus of sheep with estrus synchronized with progestagen or prostaglandin analogues. Animal Reproduction Science, 97(1-2), 25-35. doi: 10.1016/j. anireprosci.2006.01.001
Garoussi, M. T., Mavadati, O., Bahonar, M., & Ragh, M. J. (2020). The effect of medroxyprogesterone acetate with or without eCG on conception rate of fat-tail ewes in out of breeding season. Tropical Animal Health and Production, 52(4), 1617-1622. doi: 10.1007/s11250-019-02159-8
Gonzalez-Bulnes, A., Menchaca, A., Martin, G. B., & Martinez-Ros, P. (2020). Seventy years of progestagen treatments for management of the sheep oestrous cycle: where we are and where we should go. Reproduction, Fertility and Development, 32(5), 441-452. doi: 10.1071/RD18477
Letelier, C. A., Contreras-Solisa, I., García-Fernández, R. A., Sánchez, M. A., García-Palencia, P., Sánchez, B., Gonzalez-Bulnes, A. (2011). Effects of oestrus induction with progestagens or prostaglandin analogues on ovarian and pituitary function in sheep. Animal Reproduction Science, 126(1-2), 61-69. doi: 10.1016/j.anireprosci.2011.04.012
Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25(4), 402-408. doi: 10.1006/meth. 2001.1262
Ma, W., Song, H., Das, S. K., Paria, B. C., & Dey, S. K. (2003). Estrogen is a critical determinant that specifies the duration of the window of uterine receptivity for implantation. Proceedings of the National Academy of Sciences, 100(5), 2963-2968. doi: 10.1073/pnas.0530162100
Martinez-Ros, P., & Gonzalez-Bulnes, A. (2019). Efficiency of CIDR-based protocols including GnRH instead of eCG for estrus synchronization in sheep. Animals, 9(4), 1-11. doi: 10.3390/ani9040146
Martinez-Ros, P., Rios-Abellan, A., & Gonzalez-Bulnes, A. (2019). Influence of progesterone-treatment length and eCG administration on appearance of estrous behavior, ovulatory success and fertility in sheep. Animals, 9(1), 1-9. doi: 10.3390/ani9010009
Nakafeero, A., Hassen, A., & Lehloenya, K. C. (2020). Investigation of ram effect and eCG usage in progesterone based oestrous synchronization protocols on fertility of ewes following fixed time artificial insemination. Small Ruminant Research, 183, 106034. doi: 10.1016/j.smallrumres.2019.106034
Okumu, L. A., Forde, N., Fahey, A. G., Fitzpatrick, E., Roche, J. F., Crowe, M. A., & Lonergan, P. (2010). The effect of elevated progesterone and pregnancy status on mRNA expression and localisation of progesterone and oestrogen receptors in the bovine uterus. Reproduction, 140(1), 143-153. doi: 10.15 30/REP-10-0113
Robertshaw, I., Blan, F., & Das, S. K. (2016). Mechanisms of uterine estrogen signaling during early pregnancy in mice: an update Journal of Molecular Endocrinology, 56(3), 127-138. doi: 10.1530/JME-15-0300
Rosasco, S. L., Beard, J. K., Hallford, D. M., & Summers, A. F. (2019). Evaluation of estrous synchronization protocols on ewe reproductive efficiency and profitability. Animal Reproduction Science, 210, 106191. doi: 10.1016/j.anireprosci.2019.106191
Rosenfeld, C. S., Han, C., Alexenko, A. P., Spencer, T. E., & Roberts, R. M. (2002). Expression of interferon receptor subunits, IFNAR1 and IFNAR2, in the ovine uterus. Biology of Reproduction, 67(3), 847-853. doi: 10.1095/biolreprod.102.004267
Rostami, B., Hajizadeh, R., Shahir, M. H., & Aliyari, D. (2017). The effect of post-mating hCG or progesterone administration on reproductive performance of Afshari × Booroola-Merino crossbred ewes. Tropical Animal Health and Production, 49(2), 245-250. doi: 10.1007/s11250-016-1183-6
Ruiz-González, I., Sánchez, M. A., García-Fernández, R. A., García-Palencia, P., Sánchez, B., Letelier, C. A., Flores, J. M. (2011). Endometrial expression of IFNAR-1 and oxytocin receptor (OTR) is not improved by prostaglandin analogues when compared to progestagens in ewes. Reproduction in Domestic Animals, 47(2), 274-280. doi: 10.1111/j.1439-0531.2011.01852.x
Ruiz-González, I., Sánchez M. A., García-Palencia P., Sánchez B., García-Fernández, R. A., González-Bulnes A., & Flores, J. M. (2012). Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization. Animal Reproduction Science, 133(1), 93-100. doi: 10.1016/j.anireprosci.2012.06.015
Swelum, A. A., Alowaimer, A. N., & Abouheif, M. A. (2015). Use of fluorogestone acetate sponges or controlled internal drug release for estrus synchronization in ewes: effects of hormonal profiles and reproductive performance. Theriogenology, 84(4), 498-503. doi: 10.1016/j.theriogenology.2015.03.018
Takahashi, H., Haneda, S., Kayano, M., & Matsui, M. (2016). Differences in progesterone concentrations and mRNA expressions of progesterone receptors in bovine endometrial tissue between the uterine horns ipsilateral and contralateral to the corpus luteum. Journal of Veterinary Medical Science, 78(4), 613-618. doi: 10.1292/jvms.15-0366
Tortorella, R. D., Ferreira, R., Santos, J. T., Andrade, O. S., Neto, Barreta, M. H., Oliveira, J. F.,... Neves, J. P. (2013). The effect of equine chorionic gonadotropin on follicular size, luteal volume, circulating progesterone concentrations, and pregnancy rates in anestrous beef cows treated with a novel fixed-time artificial insemination protocol. Theriogenology, 79(8), 1204-1209. doi: 10.1016/j.theriogenology. 2013.02.019
Anjos, D. S. dos, Vital, A. F., Lainetti, P. F., Leis, A. F., F., Dalmolin, F., Elias, F., Fonseca-Alves, C. E. (2019). Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis. Veterinary Science, 6(1), 1-12. doi: 10.3390/vetsci6010003
Araujo, R. R., Ginther, O. J., Ferreira, J. C., Palhão, M. M., Beg, M. A., & Wiltbank, M. C. (2009). Role of follicular estradiol-17beta in timing of luteolysis in heifers. Biology of Reproduction, 81(2), 426-437. doi: 10.1095/biolreprod.108.073825
Arsoy, D., & Sağmanlıgil, V. (2018). Reproductive cycles in white Karaman ewes: comparison of ovarian hormone secretion and reproductive behavior in non-pregnant and pregnant ewes in semi-intensive conditions. Acta Scientiarum. Animal Sciences, 40(1), 1-7. doi: 10.4025/actascianimsci.v40i1.39908
Bartlewski, P. M., Baby, T. E., & Giffin, J. L. (2011). Reproductive cycles in sheep. Animal Reproduction Science, 124(3-4), 259-268. doi: 10.1016/j.anireprosci.2011.02.024
Bartlewski, P. M., Beard, A. P., Cook, S. J., Chandolia, R. K, Honaramooz, A., & Rawlings, N. C. (1999). Ovarian antral follicular dynamics and their relationships with endocrine variables throughout the oestrous cycle in breeds of sheep differing in prolificacy. Journal of Reproduction and Fertility, 115(1), 111-124. doi: 10.1530/jrf.0.1150111
Bazer, F. W. (2013). Pregnancy recognition signaling mechanisms in ruminants and pigs. Journal of Animal Science and Biotechnology, 4(1), 1-10. doi: 10.1186/2049-1891-4-23
Bazer, F. W., Spencer, T. E., & Ott, T. L. (1997). Interferon tau: a novel pregnancy recognition signal. American Journal of Reproductive Immunology, 37(6), 412-20. doi: 10.1111/j.1600-0897.1997.tb0025 3.x
Bragança, G. M., Batista, R. I. T. P., Souza-Fabjan, J. M. G., Alfradique, V. A. P., Arashiro, E. K. N., Pinto, P. H. N.,… Brandão, F. Z. (2021). Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep. Domestic Animal Endocrinology, 74, 106518. doi: 10.1016/j.domaniend.2020.106518
Brooks, K., & Spencer, T. (2015). Biological roles of interferon tau (IFNT) and type I IFN receptors in elongation of the ovine conceptus. Biology of Reproduction, 92(2), 1-10. doi: 1095/biolreprod.114.1241 56
Cosentino, I. O., Balaro, M. F. A., Arashiro, E. K. N., Santos, J. D. R., Carvalho, A. B. S., Clariget, R. P.,… Brandão, F. Z. (2019). Hormonal protocols for early resynchronization of ovulation in ewes: The use of progestagens, eCG, and inclusion of early pregnancy diagnosis with color Doppler ultrasound. Theriogenology, 133(15), 113-118. doi: 10.1016/j.theriogenology.2019.04.033
Gao, X., Zhang Q., Zhu, M., Li, X., Guo, Y., Pang, J.,… Wang, F. (2019). Effects of l-arginine on endometrial estrogen receptor α/β and progesterone receptor expression in nutrient-restricted sheep. Theriogenology, 138(15), 137-144. doi: 10.1016/j.theriogenology.2019.07.018
García-Palencia, P., Sánchez, M. A., Nieto, A., Vilar, M. P., González, M., Veiga-Lopez, A.,… Flores, J. M. (2007). Sex steroid receptor expression in the oviduct and uterus of sheep with estrus synchronized with progestagen or prostaglandin analogues. Animal Reproduction Science, 97(1-2), 25-35. doi: 10.1016/j. anireprosci.2006.01.001
Garoussi, M. T., Mavadati, O., Bahonar, M., & Ragh, M. J. (2020). The effect of medroxyprogesterone acetate with or without eCG on conception rate of fat-tail ewes in out of breeding season. Tropical Animal Health and Production, 52(4), 1617-1622. doi: 10.1007/s11250-019-02159-8
Gonzalez-Bulnes, A., Menchaca, A., Martin, G. B., & Martinez-Ros, P. (2020). Seventy years of progestagen treatments for management of the sheep oestrous cycle: where we are and where we should go. Reproduction, Fertility and Development, 32(5), 441-452. doi: 10.1071/RD18477
Letelier, C. A., Contreras-Solisa, I., García-Fernández, R. A., Sánchez, M. A., García-Palencia, P., Sánchez, B., Gonzalez-Bulnes, A. (2011). Effects of oestrus induction with progestagens or prostaglandin analogues on ovarian and pituitary function in sheep. Animal Reproduction Science, 126(1-2), 61-69. doi: 10.1016/j.anireprosci.2011.04.012
Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25(4), 402-408. doi: 10.1006/meth. 2001.1262
Ma, W., Song, H., Das, S. K., Paria, B. C., & Dey, S. K. (2003). Estrogen is a critical determinant that specifies the duration of the window of uterine receptivity for implantation. Proceedings of the National Academy of Sciences, 100(5), 2963-2968. doi: 10.1073/pnas.0530162100
Martinez-Ros, P., & Gonzalez-Bulnes, A. (2019). Efficiency of CIDR-based protocols including GnRH instead of eCG for estrus synchronization in sheep. Animals, 9(4), 1-11. doi: 10.3390/ani9040146
Martinez-Ros, P., Rios-Abellan, A., & Gonzalez-Bulnes, A. (2019). Influence of progesterone-treatment length and eCG administration on appearance of estrous behavior, ovulatory success and fertility in sheep. Animals, 9(1), 1-9. doi: 10.3390/ani9010009
Nakafeero, A., Hassen, A., & Lehloenya, K. C. (2020). Investigation of ram effect and eCG usage in progesterone based oestrous synchronization protocols on fertility of ewes following fixed time artificial insemination. Small Ruminant Research, 183, 106034. doi: 10.1016/j.smallrumres.2019.106034
Okumu, L. A., Forde, N., Fahey, A. G., Fitzpatrick, E., Roche, J. F., Crowe, M. A., & Lonergan, P. (2010). The effect of elevated progesterone and pregnancy status on mRNA expression and localisation of progesterone and oestrogen receptors in the bovine uterus. Reproduction, 140(1), 143-153. doi: 10.15 30/REP-10-0113
Robertshaw, I., Blan, F., & Das, S. K. (2016). Mechanisms of uterine estrogen signaling during early pregnancy in mice: an update Journal of Molecular Endocrinology, 56(3), 127-138. doi: 10.1530/JME-15-0300
Rosasco, S. L., Beard, J. K., Hallford, D. M., & Summers, A. F. (2019). Evaluation of estrous synchronization protocols on ewe reproductive efficiency and profitability. Animal Reproduction Science, 210, 106191. doi: 10.1016/j.anireprosci.2019.106191
Rosenfeld, C. S., Han, C., Alexenko, A. P., Spencer, T. E., & Roberts, R. M. (2002). Expression of interferon receptor subunits, IFNAR1 and IFNAR2, in the ovine uterus. Biology of Reproduction, 67(3), 847-853. doi: 10.1095/biolreprod.102.004267
Rostami, B., Hajizadeh, R., Shahir, M. H., & Aliyari, D. (2017). The effect of post-mating hCG or progesterone administration on reproductive performance of Afshari × Booroola-Merino crossbred ewes. Tropical Animal Health and Production, 49(2), 245-250. doi: 10.1007/s11250-016-1183-6
Ruiz-González, I., Sánchez, M. A., García-Fernández, R. A., García-Palencia, P., Sánchez, B., Letelier, C. A., Flores, J. M. (2011). Endometrial expression of IFNAR-1 and oxytocin receptor (OTR) is not improved by prostaglandin analogues when compared to progestagens in ewes. Reproduction in Domestic Animals, 47(2), 274-280. doi: 10.1111/j.1439-0531.2011.01852.x
Ruiz-González, I., Sánchez M. A., García-Palencia P., Sánchez B., García-Fernández, R. A., González-Bulnes A., & Flores, J. M. (2012). Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization. Animal Reproduction Science, 133(1), 93-100. doi: 10.1016/j.anireprosci.2012.06.015
Swelum, A. A., Alowaimer, A. N., & Abouheif, M. A. (2015). Use of fluorogestone acetate sponges or controlled internal drug release for estrus synchronization in ewes: effects of hormonal profiles and reproductive performance. Theriogenology, 84(4), 498-503. doi: 10.1016/j.theriogenology.2015.03.018
Takahashi, H., Haneda, S., Kayano, M., & Matsui, M. (2016). Differences in progesterone concentrations and mRNA expressions of progesterone receptors in bovine endometrial tissue between the uterine horns ipsilateral and contralateral to the corpus luteum. Journal of Veterinary Medical Science, 78(4), 613-618. doi: 10.1292/jvms.15-0366
Tortorella, R. D., Ferreira, R., Santos, J. T., Andrade, O. S., Neto, Barreta, M. H., Oliveira, J. F.,... Neves, J. P. (2013). The effect of equine chorionic gonadotropin on follicular size, luteal volume, circulating progesterone concentrations, and pregnancy rates in anestrous beef cows treated with a novel fixed-time artificial insemination protocol. Theriogenology, 79(8), 1204-1209. doi: 10.1016/j.theriogenology. 2013.02.019
Downloads
Published
2021-08-12
How to Cite
Lima, J. de S., Costa, M. da S., Pereira Júnior, J. R., Ferreira, G. J. B. de C., Faustino, L. R., Alves, C. E. F., Oba, E., Ferreira, A. C. A., Figueiredo, J. R. de, & Silva, C. M. G. da. (2021). Comparative analysis of the hormone production and gene expression profiles in ovine uterus tissue during oestrus cycle synchronized using medroxyprogesterone acetate plus eCG and prostaglandin analogue. Semina: Ciências Agrárias, 42(6), 3321–3336. https://doi.org/10.5433/1679-0359.2021v42n6p3321
Issue
Section
Articles
License
Copyright (c) 2021 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
O Copyright dos manuscritos publicados pertence ao periódico. Como são publicados em um periódico de acesso aberto, eles estão disponíveis gratuitamente, para uso privado ou para fins educacionais e não comerciais.
A revista tem o direito de fazer, no documento original, alterações referentes às normas lingüísticas, ortografia e gramática, com o objetivo de garantir as normas padrão do idioma e a credibilidade da revista. No entanto, respeitará o estilo de escrita dos autores.
Quando necessário, alterações conceituais, correções ou sugestões serão encaminhadas aos autores. Nesses casos, o manuscrito deve ser submetido a uma nova avaliação após revisão.
A responsabilidade pelas opiniões expressas nos manuscritos é inteiramente dos autores.
