Celulases fúngicas: produção por cultivo sólido em biorreator de leito empacotado usando resíduos sólidos agroindustriais como substratos e aplicação na hidrólise de bagaço de cana
DOI:
https://doi.org/10.5433/1679-0359.2020v41n5supl1p2097Palavras-chave:
Bioetanol, Celulases, Pré-tratamento, Sacarificação, Cultivo sólido.Resumo
Celulases são essenciais para a hidrólise de materiais lignocelulósicos visando à produção de etanol de segunda geração. O cultivo em estado sólido é um processo que proporciona altas concentrações de enzimas que podem ser aplicadas nessa hidrólise. O objetivo deste trabalho foi produzir celulases pelo fungo Myceliophthora thermophila I-1D3b em biorreator de leito empacotado com bagaço de cana (BC) e farelo de trigo (FT) como substrato e avaliar a eficiência deste extrato enzimático na hidrólise de bagaço de cana in natura (BIN) e pré-tratado com ozônio, álcali e ultrassom (BOU). As condições para produção de enzimas no biorreator foram BC:FT numa proporção de 2,3:1 (m/m); 75 % de umidade em base úmida; 45 ºC; taxa de aeração 240 L h-1 e 96 h. A produção de enzimas foi avaliada pelas atividades de endoglucanase, xilanase, papel de filtro (FPU) e ?-glicosidase. Para a aplicação das enzimas, foi utilizado um planejamento de superfície de resposta central composto com 5 repetições do ponto central, tendo como fatores o volume de extrato enzimático e o tempo de hidrólise. Esse cultivo produziu as seguintes atividades enzimáticas: 723 U gss-1 de endoglucanases, 2024 U gss-1 de xilanase, 12,6 U gss-1 de FPU e 41 U gss-1 de ?-glucosidase. Os resultados da aplicação dessas enzimas na hidrólise indicaram como ótimos 7,0 ml de extrato enzimático (4,2 FPU) e 6 horas para BIN e BOU. As melhores conversões de celulose-glicose foram obtidas para a BOU, atingindo 32,1 % a 65 ºC. Em conclusão, a produção de enzimas no biorreator de leito compactado foi eficiente e o pré-tratamento BOU melhorou a hidrólise da biomassa, aumentando a eficiência de conversão de celulose em glicose.Métricas
Carregando Métricas ...
Referências
Afonso, L. C. (2012). Produção de celulases por cultivo em estado sólido e aplicação na hidrólise do bagaço de cana-de-açúcar. Dissertação de mestrado, Universidade de São Paulo, SP, Brasil.
Badhan, A. K., Chadha, B. S., Kaur, J., Saini, H. S., & Bhat, M. K. (2007). Production of multiple xylanilytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099. Bioresource Technology, 98(3), 504-510. doi: 10.1016/j.biortech.2006.02.009
Bailey, M. J., Peterbiely, P., & Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, 23(3), 257-270. doi: 10.1016/0168-1656(92)90074-J
Balat, M., Balat, H., & Öz, C. (2008). Progress in bioethanol processing. Progress in Energy and Combustion Science, 34(5), 551-573. doi: 10.1016/j.pecs.2007.11.001
Baruah, J., Nath, B. K., Sharma, R., Kumar, S., Deka, R. C., Baruah, D. C., & Kalita, E. (2018). Recent trends in the pretreatment of lignocellulosic biomass for value-added products. Frontiers in Energy Research, 6, 1-19. doi: 10.3389/fenrg.2018.00141
Bi, S., Peng, L., Chen, K., & Zhu, Z. (2016). Enhanced enzymatic saccharification of sugarcane bagasse pretreated by combining O2 and NaOH. Bioresource Technology, 214, 692-699. doi: 10.1016/j.biortech. 2016.05.041
Calado, V., & Montgomery, D. (2003). Planejamento de experimentos usando o Statistic. Rio de Janeiro: E-papers Serviços Editoriais.
Canilha, L., Chandel, A. K., Milessi, T. S. S., Antunes, F. A. F., Freitas, W. L. C., Felipe, M. G. A., & Silva, S. S. da. (2012). Bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation. Journal of Biomedicine and Biotechnology, 2012, 1-15. doi: 10.1155/2012/989572
Cantwell, B. A., Sharp, P. M., Gormley, E., & Mcconnell, D. J. (1988). Molecular cloning of bacillus b-glucanases. In J. P. Aubert, P. Beguin, & J. Millet (Eds.), Biochemistry and genetics of cellulose degradation (pp. 181-201). San Diego: Academic Press.
Casciatori, F. P., Bück, A., Thoméo, J. C., & Tsotsas, E. (2016). Two-phase and two-dimensional model describing heat and water transfer during solid-state fermentation within a packed-bed bioreactor. Chemical Engineering Journal, 287, 103-116. doi: 10.1016/j.cej.2015.10.108
Casciatori, F. P., Casciatori, P. A., & Thoméo, J. C. (2013). Cellulase production in packed bed bioreactor by solid-state fermentation. Proceedings of the European Biomass Conference and Exhibition, Copenhagen, Dinamarca, 21st. doi: 10.5071/21stEUBCE2013-3DV.1.13
Derakhti, S., Shojaosadati, S. A., Hashemi, M., & Khajeh, K. (2012). Process parameters study of α-amylase production in a packed-bed bioreactor under solid-state fermentation with possibility of temperature monitoring. Preparative Biochemistry and Biotechnology, 42(3), 203-216. doi: 10.1080/10826068.2011. 599466
Fan, L. T., Gharpuray, M. M., & Lee, Y. H. (1987). Cellulose hydrolysis biotechnology monographs. Berlin: Springer-Verlag.
Florencio, C., Badino, A. C., & Farinas, C. S. (2017). Desafios relacionados à produção e aplicação das enzimas celulolíticas na hidrólise da biomassa lignocelulósica. Química Nova, 40(9), 1082-1093. doi: 10. 21577/0100-4042.20170104
Frassatto, P. A. C., Casciatori, F. P., Thoméo, J. C., Gomes, E., Boscolo, M., & Silva, R. da. (2020). β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse. Biomass Conversion and Biorefinery, 1-11. doi: 10.1007/s13399-020-00608-1
Ghildyal, N. P., Gowthaman, M. K., Raghava Rao, K. S. M. S., & Karanth, N. G. (1994). Interaction of transport resistances with biochemical reaction in packed-bed solid-state fermentors: Effect of temperature gradients. Enzyme and Microbial Technology, 16(3), 253-257. doi: 10.1016/0141-0229(94) 90051-5
Ghose, T. K. (1987). Measurement of cellulase activities. Pure and Applied Chemistry, 59(2), 257-268. doi: 10.1351/pac198759020257
Kalogeris, E., Christakopoulos, P., Katapodis, P., Alexiou, A., Vlachou, S., Kekos, D., & Macris, B. J. (2003). Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes. Process Biochemistry, 38(7), 1099-1104. doi: 10.1016/S0032-9592(02)00242-X
Leite, R. S. R., Bocchini, D. A., Martins, E. S., Silva, D., Gomes, E., & Silva, R. da. (2007). Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluanson solid state fermentation. Applied Biochemistry and Biotechnology, 137(1-12), 281-288. doi: 10.1007/s12010-007-9058-y
Liu, C., Suna, R., Qin, M., Zhang, A., Rena, J., Xub, F.,… Wu, S. (2007). Chemical modification of ultrasound-pretreated sugarcane bagasse with maleic anhydride. Industrial Crops and Products, 26(2), 212-219. doi: 10.1016/j.indcrop.2007.03.007
Lopes, A. M., Ferreira, E. X., Fº., & Moreira, L. R. S. (2018). An update on enzymatic cocktails for lignocellulose breakdown. Journal of Applied Microbiology, 125(3), 632-645. doi: 10.1111/jam.13923
Manan, M. A., & Webb, C. (2017). Design aspects of solid state fermentation as applied to microbial bioprocessing. Journal of Applied Biotechnology & Bioengineering, 4(1), 511-532. doi: 10.15406/jabb. 2017.04.00094
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426-428. doi: 10.1021/ac60147a030
Mishima, D., Tateda, M., Ike, M., & Fujita, M. (2006). Comparative study on chemical pretreatments to accelerate enzymatic hydrolysis of aquatic macrophyte biomass used in water purification processes. Bioresource Technology, 97(16), 2166-2172. doi: 10.1016/j.biortech.2005.09.029
Mitchell, D. A., Krieger, N., & Berovic, M. (2006). Solid-state fermentation bioreactors: fundamentals, design and operation. Berlin: Springer-Verlag.
Mitchell, D. A., Pandey, A., Sangsurasak, P., & Krieger, N. (1999). Scale-up strategies for packed bed bioreactors for solid state fermentation. Process Biochemistry, 35(1-2), 167-178. doi: 10.1016/S0032-9592(99)00048-5
Molina, G., Contesini, F. J., Melo, C. R. R. de, Sato, H. H., & Pastore, G. M. (2016). β-Glucosidase from Aspergillus. In V. K. Gupta (Eds.), New and future developments in microbial biotechnology and bioengineering: aspergillus system properties and applications (pp. 155-169). Amsterdã: Elsevier.
Montgomery, D. C. (2001). Design and analysis of experiments. New York: John Wiley & Sons.
Moretti, M. M. S., Bocchini-Martins, D. A., Silva, R. da, Rodrigues, A., Sette, L. D., & Gomes, E. (2012). Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation. Brazilian Journal of Microbiology, 43(3), 1062-1071. doi: 10.1590/S1517-83822012000300032
Oliveira Rodrigues, P. de, Pereira, J. C., Queiroz, D., Gurgel, L. V. A., Pasquini, D., & Baffi, M. A. (2017). Synergistic action of an Aspergillus (hemi-) cellulolytic consortium on sugarcane bagasse saccharification. Industrial Crops and Products, 109(1), 173-181. doi: 10.1016/j.indcrop.2017.08.031
Pereira, J. C., Travaini, R., Marques, N. P., Bolado-Rodríguez, S., & Martins, D. A. B. (2016). Saccharification of ozonated sugarcane bagasse using enzymes from Myceliophthora thermophila JCP 1-4 for sugars release and etanol production. Bioresource Technology, 204, 122-129. doi: 10.1016/j. biortech.2015.12.064
Perez, C. L., Casciatori, F. P., & Thoméo, J. C. (2019). Strategies for scaling-up packed-bed bioreactors for solid-state fermentation: the case of cellulolytic enzymes production by a thermophilic fungus. Chemical Engineering Journal, 361, 1142-1151. doi: 10.1016/j.cej.2018.12.169
Perrone, O. M., Colombari, F. M., Rossi, J. S., Moretti, M. M. S., Bordignon, S. E., Nunes, C. C. C., Silva, R. da. (2016). Ozonolysis combined with ultrasound as a pretreatment of sugarcane bagasse: effect on the enzymatic saccharification and the physical and chemical characteristics of the substrate. Bioresource Technology, 218, 69-76. doi: 10.1016/j.biortech.2016.06.072
Plácido, J., & Capareda, S. (2014). Analysis of alkali ultrasonication pretreatment in bioethanol production from cotton gin trash using FT-IR spectroscopy and principal component analysis. Bioresources and Bioprocessing, 1(23), 1-9. doi: 10.1186/s40643-014-0023-7
Sandgren, M., Stahlberg, J., & Mitchinson, C. (2005). Structural and biochemical studies of GH family 12 cellulases: improved thermal stability, and ligand complexes. Progress in Biophysics and Molecular Biology, 89(3), 246-291. doi: 10.1016/j.pbiomolbio.2004.11.002
Silva, R. da, Lago, E. S., Merheb, C. W., Macchione, M. M., & Park, Y. K. (2005). Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus Miehe. Brazilian Journal of Microbiology, 36(3), 235-241. doi: 10.1590/S1517-83822005000300006
Sluiter, A., Hames, B., Ruiz, R. O., Scarlata, C., Sluiter, J., Templeton, D., & Crocker, D. (2008). Determination of structural carbohydrates and lignin in biomass. Technical Report NREL, TP-510-42618). Biomass Anal. Golden, CO: National Renewable Energy Laboratory Technol. (Recuperado de https://www.nrel.gov/biomass/pdfs/42618.pdf
Soni, R., Nazir, A., Chadha B. S., & Saini, H. S. (2008). Novel sources of fungal cellulases for efficient deinking of composite paper wast. Bioresources, 3(1), 234-246.
Souza-Corrêa, J. A., Oliveira, C., Nascimento, V. M., Wolf, L. D., Gómez, E. O., Rocha, G. J. M., & Amorim, J. (2014). Atmospheric pressure plasma pretreatment of sugarcane bagasse: the influence of biomass particle size in the ozonation process. Applied Biochemistry and Biotechnology, 172(3), 1663-1672. doi: 10.1007/s12010-013-0609-0
Souza-Corrêa, J. A., Ridenti, M. A., Oliveira, C., Araújo, S. R., & Amorim, J. (2013). Decomposition of lignin from sugar cane bagasse during ozonation process monitored by optical and mass spectrometries. The Journal of Physical Chemistry, 117(11), 3110-3119. doi: 10.1021/jp3121879
Sun, J. X., Sun, R., Sun, X. F., & Su, Y. (2004). Fractional and physico-chemical characterization of hemicelluloses from ultrasonic irradiated sugarcane bagasse. Carbohydrate Research, 339(2), 291-300. doi: 10.1016/j.carres.2003.10.027
Travaini, R., Otero, M. D. M., Coca, M., Silva, R. da, & Bolado, S. (2013). Sugarcane bagasse ozonolysis pretreatment: effect on enzymatic digestibility and inhibitory compound formation. Bioresource Technology, 133, 332-339. doi: 10.1016/j.biortech.2013.01.133
Velmurugan, R., & Muthukumar, K. (2011). Utilization of sugarcane bagasse for bioethanol production: sono-assisted acid hydrolysis approach. Bioresource Technology, 102(14), 7119-7123. doi: 10.1016/j. biortech.2011.04.045
Yang, H., Yan, R., Chen, H., Lee, D. H., & Zheng, C. (2007). Characteristics of hemicellulose, cellulose and lignin pyrolysis. Fuel, 86(12-13), 1781-1788. doi: 10.1016/j.fuel.2006.12.013
Zanelato, A. I., Shiota, V. M., Gomes, E., & Thoméo, J. C. (2012). Endoglucanase production with the newly isolated Myceliophthora sp. I-1D3b in a packed bed solid state fermentor. Brazilian Journal of Microbiology, 43(4), 1536-1544. doi: 10.1590/S1517-83822012000400038
Badhan, A. K., Chadha, B. S., Kaur, J., Saini, H. S., & Bhat, M. K. (2007). Production of multiple xylanilytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099. Bioresource Technology, 98(3), 504-510. doi: 10.1016/j.biortech.2006.02.009
Bailey, M. J., Peterbiely, P., & Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, 23(3), 257-270. doi: 10.1016/0168-1656(92)90074-J
Balat, M., Balat, H., & Öz, C. (2008). Progress in bioethanol processing. Progress in Energy and Combustion Science, 34(5), 551-573. doi: 10.1016/j.pecs.2007.11.001
Baruah, J., Nath, B. K., Sharma, R., Kumar, S., Deka, R. C., Baruah, D. C., & Kalita, E. (2018). Recent trends in the pretreatment of lignocellulosic biomass for value-added products. Frontiers in Energy Research, 6, 1-19. doi: 10.3389/fenrg.2018.00141
Bi, S., Peng, L., Chen, K., & Zhu, Z. (2016). Enhanced enzymatic saccharification of sugarcane bagasse pretreated by combining O2 and NaOH. Bioresource Technology, 214, 692-699. doi: 10.1016/j.biortech. 2016.05.041
Calado, V., & Montgomery, D. (2003). Planejamento de experimentos usando o Statistic. Rio de Janeiro: E-papers Serviços Editoriais.
Canilha, L., Chandel, A. K., Milessi, T. S. S., Antunes, F. A. F., Freitas, W. L. C., Felipe, M. G. A., & Silva, S. S. da. (2012). Bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation. Journal of Biomedicine and Biotechnology, 2012, 1-15. doi: 10.1155/2012/989572
Cantwell, B. A., Sharp, P. M., Gormley, E., & Mcconnell, D. J. (1988). Molecular cloning of bacillus b-glucanases. In J. P. Aubert, P. Beguin, & J. Millet (Eds.), Biochemistry and genetics of cellulose degradation (pp. 181-201). San Diego: Academic Press.
Casciatori, F. P., Bück, A., Thoméo, J. C., & Tsotsas, E. (2016). Two-phase and two-dimensional model describing heat and water transfer during solid-state fermentation within a packed-bed bioreactor. Chemical Engineering Journal, 287, 103-116. doi: 10.1016/j.cej.2015.10.108
Casciatori, F. P., Casciatori, P. A., & Thoméo, J. C. (2013). Cellulase production in packed bed bioreactor by solid-state fermentation. Proceedings of the European Biomass Conference and Exhibition, Copenhagen, Dinamarca, 21st. doi: 10.5071/21stEUBCE2013-3DV.1.13
Derakhti, S., Shojaosadati, S. A., Hashemi, M., & Khajeh, K. (2012). Process parameters study of α-amylase production in a packed-bed bioreactor under solid-state fermentation with possibility of temperature monitoring. Preparative Biochemistry and Biotechnology, 42(3), 203-216. doi: 10.1080/10826068.2011. 599466
Fan, L. T., Gharpuray, M. M., & Lee, Y. H. (1987). Cellulose hydrolysis biotechnology monographs. Berlin: Springer-Verlag.
Florencio, C., Badino, A. C., & Farinas, C. S. (2017). Desafios relacionados à produção e aplicação das enzimas celulolíticas na hidrólise da biomassa lignocelulósica. Química Nova, 40(9), 1082-1093. doi: 10. 21577/0100-4042.20170104
Frassatto, P. A. C., Casciatori, F. P., Thoméo, J. C., Gomes, E., Boscolo, M., & Silva, R. da. (2020). β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse. Biomass Conversion and Biorefinery, 1-11. doi: 10.1007/s13399-020-00608-1
Ghildyal, N. P., Gowthaman, M. K., Raghava Rao, K. S. M. S., & Karanth, N. G. (1994). Interaction of transport resistances with biochemical reaction in packed-bed solid-state fermentors: Effect of temperature gradients. Enzyme and Microbial Technology, 16(3), 253-257. doi: 10.1016/0141-0229(94) 90051-5
Ghose, T. K. (1987). Measurement of cellulase activities. Pure and Applied Chemistry, 59(2), 257-268. doi: 10.1351/pac198759020257
Kalogeris, E., Christakopoulos, P., Katapodis, P., Alexiou, A., Vlachou, S., Kekos, D., & Macris, B. J. (2003). Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes. Process Biochemistry, 38(7), 1099-1104. doi: 10.1016/S0032-9592(02)00242-X
Leite, R. S. R., Bocchini, D. A., Martins, E. S., Silva, D., Gomes, E., & Silva, R. da. (2007). Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluanson solid state fermentation. Applied Biochemistry and Biotechnology, 137(1-12), 281-288. doi: 10.1007/s12010-007-9058-y
Liu, C., Suna, R., Qin, M., Zhang, A., Rena, J., Xub, F.,… Wu, S. (2007). Chemical modification of ultrasound-pretreated sugarcane bagasse with maleic anhydride. Industrial Crops and Products, 26(2), 212-219. doi: 10.1016/j.indcrop.2007.03.007
Lopes, A. M., Ferreira, E. X., Fº., & Moreira, L. R. S. (2018). An update on enzymatic cocktails for lignocellulose breakdown. Journal of Applied Microbiology, 125(3), 632-645. doi: 10.1111/jam.13923
Manan, M. A., & Webb, C. (2017). Design aspects of solid state fermentation as applied to microbial bioprocessing. Journal of Applied Biotechnology & Bioengineering, 4(1), 511-532. doi: 10.15406/jabb. 2017.04.00094
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426-428. doi: 10.1021/ac60147a030
Mishima, D., Tateda, M., Ike, M., & Fujita, M. (2006). Comparative study on chemical pretreatments to accelerate enzymatic hydrolysis of aquatic macrophyte biomass used in water purification processes. Bioresource Technology, 97(16), 2166-2172. doi: 10.1016/j.biortech.2005.09.029
Mitchell, D. A., Krieger, N., & Berovic, M. (2006). Solid-state fermentation bioreactors: fundamentals, design and operation. Berlin: Springer-Verlag.
Mitchell, D. A., Pandey, A., Sangsurasak, P., & Krieger, N. (1999). Scale-up strategies for packed bed bioreactors for solid state fermentation. Process Biochemistry, 35(1-2), 167-178. doi: 10.1016/S0032-9592(99)00048-5
Molina, G., Contesini, F. J., Melo, C. R. R. de, Sato, H. H., & Pastore, G. M. (2016). β-Glucosidase from Aspergillus. In V. K. Gupta (Eds.), New and future developments in microbial biotechnology and bioengineering: aspergillus system properties and applications (pp. 155-169). Amsterdã: Elsevier.
Montgomery, D. C. (2001). Design and analysis of experiments. New York: John Wiley & Sons.
Moretti, M. M. S., Bocchini-Martins, D. A., Silva, R. da, Rodrigues, A., Sette, L. D., & Gomes, E. (2012). Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation. Brazilian Journal of Microbiology, 43(3), 1062-1071. doi: 10.1590/S1517-83822012000300032
Oliveira Rodrigues, P. de, Pereira, J. C., Queiroz, D., Gurgel, L. V. A., Pasquini, D., & Baffi, M. A. (2017). Synergistic action of an Aspergillus (hemi-) cellulolytic consortium on sugarcane bagasse saccharification. Industrial Crops and Products, 109(1), 173-181. doi: 10.1016/j.indcrop.2017.08.031
Pereira, J. C., Travaini, R., Marques, N. P., Bolado-Rodríguez, S., & Martins, D. A. B. (2016). Saccharification of ozonated sugarcane bagasse using enzymes from Myceliophthora thermophila JCP 1-4 for sugars release and etanol production. Bioresource Technology, 204, 122-129. doi: 10.1016/j. biortech.2015.12.064
Perez, C. L., Casciatori, F. P., & Thoméo, J. C. (2019). Strategies for scaling-up packed-bed bioreactors for solid-state fermentation: the case of cellulolytic enzymes production by a thermophilic fungus. Chemical Engineering Journal, 361, 1142-1151. doi: 10.1016/j.cej.2018.12.169
Perrone, O. M., Colombari, F. M., Rossi, J. S., Moretti, M. M. S., Bordignon, S. E., Nunes, C. C. C., Silva, R. da. (2016). Ozonolysis combined with ultrasound as a pretreatment of sugarcane bagasse: effect on the enzymatic saccharification and the physical and chemical characteristics of the substrate. Bioresource Technology, 218, 69-76. doi: 10.1016/j.biortech.2016.06.072
Plácido, J., & Capareda, S. (2014). Analysis of alkali ultrasonication pretreatment in bioethanol production from cotton gin trash using FT-IR spectroscopy and principal component analysis. Bioresources and Bioprocessing, 1(23), 1-9. doi: 10.1186/s40643-014-0023-7
Sandgren, M., Stahlberg, J., & Mitchinson, C. (2005). Structural and biochemical studies of GH family 12 cellulases: improved thermal stability, and ligand complexes. Progress in Biophysics and Molecular Biology, 89(3), 246-291. doi: 10.1016/j.pbiomolbio.2004.11.002
Silva, R. da, Lago, E. S., Merheb, C. W., Macchione, M. M., & Park, Y. K. (2005). Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus Miehe. Brazilian Journal of Microbiology, 36(3), 235-241. doi: 10.1590/S1517-83822005000300006
Sluiter, A., Hames, B., Ruiz, R. O., Scarlata, C., Sluiter, J., Templeton, D., & Crocker, D. (2008). Determination of structural carbohydrates and lignin in biomass. Technical Report NREL, TP-510-42618). Biomass Anal. Golden, CO: National Renewable Energy Laboratory Technol. (Recuperado de https://www.nrel.gov/biomass/pdfs/42618.pdf
Soni, R., Nazir, A., Chadha B. S., & Saini, H. S. (2008). Novel sources of fungal cellulases for efficient deinking of composite paper wast. Bioresources, 3(1), 234-246.
Souza-Corrêa, J. A., Oliveira, C., Nascimento, V. M., Wolf, L. D., Gómez, E. O., Rocha, G. J. M., & Amorim, J. (2014). Atmospheric pressure plasma pretreatment of sugarcane bagasse: the influence of biomass particle size in the ozonation process. Applied Biochemistry and Biotechnology, 172(3), 1663-1672. doi: 10.1007/s12010-013-0609-0
Souza-Corrêa, J. A., Ridenti, M. A., Oliveira, C., Araújo, S. R., & Amorim, J. (2013). Decomposition of lignin from sugar cane bagasse during ozonation process monitored by optical and mass spectrometries. The Journal of Physical Chemistry, 117(11), 3110-3119. doi: 10.1021/jp3121879
Sun, J. X., Sun, R., Sun, X. F., & Su, Y. (2004). Fractional and physico-chemical characterization of hemicelluloses from ultrasonic irradiated sugarcane bagasse. Carbohydrate Research, 339(2), 291-300. doi: 10.1016/j.carres.2003.10.027
Travaini, R., Otero, M. D. M., Coca, M., Silva, R. da, & Bolado, S. (2013). Sugarcane bagasse ozonolysis pretreatment: effect on enzymatic digestibility and inhibitory compound formation. Bioresource Technology, 133, 332-339. doi: 10.1016/j.biortech.2013.01.133
Velmurugan, R., & Muthukumar, K. (2011). Utilization of sugarcane bagasse for bioethanol production: sono-assisted acid hydrolysis approach. Bioresource Technology, 102(14), 7119-7123. doi: 10.1016/j. biortech.2011.04.045
Yang, H., Yan, R., Chen, H., Lee, D. H., & Zheng, C. (2007). Characteristics of hemicellulose, cellulose and lignin pyrolysis. Fuel, 86(12-13), 1781-1788. doi: 10.1016/j.fuel.2006.12.013
Zanelato, A. I., Shiota, V. M., Gomes, E., & Thoméo, J. C. (2012). Endoglucanase production with the newly isolated Myceliophthora sp. I-1D3b in a packed bed solid state fermentor. Brazilian Journal of Microbiology, 43(4), 1536-1544. doi: 10.1590/S1517-83822012000400038
Downloads
Publicado
2020-08-07
Como Citar
Frassatto, P. A. C., Casciatori, F. P., Thoméo, J. C., Gomes, E., Boscolo, M., & Silva, R. da. (2020). Celulases fúngicas: produção por cultivo sólido em biorreator de leito empacotado usando resíduos sólidos agroindustriais como substratos e aplicação na hidrólise de bagaço de cana. Semina: Ciências Agrárias, 41(5supl1), 2097–2116. https://doi.org/10.5433/1679-0359.2020v41n5supl1p2097
Edição
Seção
Artigos
Licença
Copyright (c) 2020 Semina: Ciências Agrárias
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial 4.0 International License.
Os Direitos Autorais para artigos publicados são de direito da revista. Em virtude da aparecerem nesta revista de acesso público, os artigos são de uso gratuito, com atribuições próprias, em aplicações educacionais e não-comerciais.
A revista se reserva o direito de efetuar, nos originais, alterações de ordem normativa, ortográfica e gramatical, com vistas a manter o padrão culto da língua e a credibilidade do veículo. Respeitará, no entanto, o estilo de escrever dos autores.
Alterações, correções ou sugestões de ordem conceitual serão encaminhadas aos autores, quando necessário. Nesses casos, os artigos, depois de adequados, deverão ser submetidos a nova apreciação.
As opiniões emitidas pelos autores dos artigos são de sua exclusiva responsabilidade.
