Hemotropic mycoplasmas in bats from forest fragments, state of Paraná, southern Brazil
DOI:
https://doi.org/10.5433/1679-0359.2022v43n1p431Palabras clave:
Bats, Hemotropic mycoplasmas, Hemoplasmas, Mycoplasma sp.Resumen
The order Chiroptera is the second largest group of mammals with bats being identified as reservoir of several viral zoonoses, although, little is known about their role in other groups of pathogens, including hemotropic Mycoplasma spp. To date, hemoplasma species have been found infecting several species of bats with high genetic diversity between 16S rRNA gene sequences. On this study, we aimed to identify the occurrence and characterize 16S and 23S rRNA genes of hemoplasma species in four bats species (Artibeus lituratus, Carollia perspicillata, Sturnira lilium and Sturnira tildae) from forest fragments in Paraná State, southern Brazil, using PCR-based assays. Spleen tissue samples were collected, DNA extracted and further screened by a pan hemoplasma PCR assay. All samples consistently amplified the mammal endogenous gapdh gene. One out of 15 (6.66%; 95% CI: 0.2-31%) bats tested positive for hemotropic Mycoplasma sp. by the PCR assay targeting the 16S rRNA gene. Sequencing of the 16S rRNA gene fragment from the hemoplasma-positive bat showed 99.14% identity with hemotropic Mycoplasma sp. detected in Sturnira parvidens from Belize. Sequencing of the 23S rRNA gene fragment from the hemoplasma-positive bat showed 86.17% identity with Candidatus Mycoplasma haemosphiggurus’ detected in orange spined hairy dwarf porcupines (Sphiggurus villosus) from Southern Brazil.Métricas
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Alabí, A. S., Monti, G., Otth, C., Sepulveda-García, P., Sánchez-Hidalgo, M., Mello, V. V. C. de,… Müller A. (2020). Molecular survey and genetic diversity of hemoplasmas in rodents from Chile. Microorganisms, 8(10), 1493. doi: 10.3390/microorganisms8101493
Becker, D. J., Speer, K. A., Brown, A. M., Fenton, M. B., Washburne, A. D., Altizer, S.,… Volokh, D. V. (2020). Ecological and evolutionary drivers of haemoplasma infection and bacterial genotype sharing in a Neotropical bat community. Molecular Ecology, 29(8), 1534-1549. doi: 10.1111/mec.15422
Birkenheuer, A. J., Levy, M. G., & Breitschwerdt, E. B. (2003). Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples. Journal of Clinical Microbiology, 41(9), 4172-4177. doi: 10.1128/JCM.41.9.4172-4177. 2003
Brook, C. E., & Dobson, A. P. (2014). Bats as ‘special’ reservoirs for emerging zoonotic pathogens. Trends in Microbiology, 23(33), 172-180. doi: 10.1016/j.tim.2014.12.004
Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9(88), 772. doi: 10.1038/nmeth.2109
Descloux, E., Mediannikov, O., Gourinat, A., Colot, J., Chauvet, M., Mermoud, I.,… Raoult, D. (2020). Flying fox haemolytic fever, description of a new zoonosis caused by “Candidatus Mycoplasma haemohominis”. Clinical Infectious Diseases, 73(7), 1445-1453. doi: 10.1093/cid/ciaa1648
Di Cataldo, S., Kamani, J., Cevidanes, A., Msheliza, E. G., & Millán, J. (2020). Hemotropic mycoplasmas in bats captured near human settlements in Nigeria. Comparative Immunology, Microbiology & Infectious Diseases, 70(1), 101448. doi: 10.1016/j.cimid.2020.101448
Drummond, A. J., Suchard, M. A., Xie, D., & Rambaut, A. (2012). Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution, 29(8), 1969-1973. doi: 10.1093/molbev/mss075
Hoelzle, K., Winkler, M., Kramer, M. M., Wittenbrink, M. M., Dieckmann, S. M., & Hoelzle, L. E. (2011). Detection of Candidatus Mycoplasma haemobos in cattle with anaemia. Veterinary Journal, 187(3), 408-410. doi: 10.1016/j.tvjl.2010.01.016
Ikeda, P., Seki, M., Carrasco, A., Rudiak, L., Miranda, J., Gonçalves, S. M. M.,... André, M. R. (2017). Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil. Epidemiology & Infection, 145(10), 2038-2052. doi: 10.1017/S0950268817000966
Katoh, K., & Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30(4), 772-780. doi: 10. 1093/molbev/mst010
Machado, C. A. L., Vidotto, O., Conrado, F. O., Santos, N. J. R., Valente, J. D. M., Barbosa, I. C.,... Vieira, R. F. C. (2017). Mycoplasma ovis infection in goat farms from northeastern Brazil. Comparative Immunology, Microbiology and Infectious Diseases, 55(1), 1-5. doi: 10.1016/j.cimid.2017.08.004
Mascarelli, P. E., Keel, M. K., Yabsley, M., Last, L. A., Breitschwerdt, E. B., & Maggi, R. G. (2014). Hemotropic mycoplasmas in little brown bats (Myotis lucifugus). Parasites & Vectors, 7(1), 117. doi: 10.1186/1756-3305-7-117
Millán, J., Di Cataldo, S., Volokhov, D. V., & Becker, D. J. (2020). Worldwide occurrence of haemoplasmas in wildlife: Insights into the patterns of infection, transmission, pathology and zoonotic potential. Transboundary and Emerging Diseases, 00, 1-21. doi: 10.1111/tbed.13932
Millán, J., López-Roig, M., Delicado, V., Serra-Cobo, J., & Esperón, F. (2015). Widespread infection with hemotropic mycoplasmas in bats in Spain, including a hemoplasma closely related to “Candidatus Mycoplasma hemohominis”. Comparative Immunology, Microbiology and Infectious Diseases, 39(1), 9-12. doi: 10.1016/j.cimid.2015.01.002
Mongruel, A. C. B., Spanhol, V. C., Valente, J. D. M., Porto, P. P., Ogawa, L., Otomura, F.,... Vieira, R. F. C. (2020). Survey of vector-borne and nematode parasites involved in the etiology of anemic syndrome in sheep from Southern Brazil. Revista Brasileira de Parasitologia Veterinária, 29(3), e007320. doi: 10. 1590/S1984-29612020062
Reis, N. R., Peracchi, A. L., Batista, C. B., Lima, I. P. de, & Pereira, A. D. (2017). História natural dos morcegos brasileiros: chave de identificação de espécies. Rio de Janeiro: Technical Books.
Santos, L. C., Vidotto, O., Santos, N. J. R., Ribeiro, J., Pellizzaro, M., Santos, A. P.,... Biondo, A. W. (2020). Hemotropic mycoplasmas (hemoplasmas) in free-ranging bats from Southern Brazil. Comparative Immunology, Microbiology and Infectious Diseases, 69(1), 101416. doi: 10.1016/j.cimid.2020.101416
Schipper, J., Chanson, J. S., Chiozza, F., Cox, N. A., Hoffmann, M., Katariya, V.,… Young, B. E. (2008). The status of the world’s land and marine mammals: diversity, threat, and knowledge. Science, 322(5899), 255-230. doi: 10.1126/science.1165115
Sela, I., Ashkenazy, H., Katoh, K., & Pupko, T. (2015). Guidance 2: accurate detection of unreliable alignment regions accounting for the uncertainty of multiple parameters. Nucleic Acids Research, 43(1), 7-14. doi: 10.1093/nar/gkv318
Volokhov, D. V., Becker, D. J., Bergner, L. M., Camus, M. S., Orton, R. J., Chizhikov, V. E.,… Streicker, D. G. (2017). Novel hemotropic mycoplasmas are widespread and genetically diverse in vampire bats. Epidemiology & Infection, 145(15), 3154-3167. doi: 10.1017/S095026881700231X
Becker, D. J., Speer, K. A., Brown, A. M., Fenton, M. B., Washburne, A. D., Altizer, S.,… Volokh, D. V. (2020). Ecological and evolutionary drivers of haemoplasma infection and bacterial genotype sharing in a Neotropical bat community. Molecular Ecology, 29(8), 1534-1549. doi: 10.1111/mec.15422
Birkenheuer, A. J., Levy, M. G., & Breitschwerdt, E. B. (2003). Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples. Journal of Clinical Microbiology, 41(9), 4172-4177. doi: 10.1128/JCM.41.9.4172-4177. 2003
Brook, C. E., & Dobson, A. P. (2014). Bats as ‘special’ reservoirs for emerging zoonotic pathogens. Trends in Microbiology, 23(33), 172-180. doi: 10.1016/j.tim.2014.12.004
Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9(88), 772. doi: 10.1038/nmeth.2109
Descloux, E., Mediannikov, O., Gourinat, A., Colot, J., Chauvet, M., Mermoud, I.,… Raoult, D. (2020). Flying fox haemolytic fever, description of a new zoonosis caused by “Candidatus Mycoplasma haemohominis”. Clinical Infectious Diseases, 73(7), 1445-1453. doi: 10.1093/cid/ciaa1648
Di Cataldo, S., Kamani, J., Cevidanes, A., Msheliza, E. G., & Millán, J. (2020). Hemotropic mycoplasmas in bats captured near human settlements in Nigeria. Comparative Immunology, Microbiology & Infectious Diseases, 70(1), 101448. doi: 10.1016/j.cimid.2020.101448
Drummond, A. J., Suchard, M. A., Xie, D., & Rambaut, A. (2012). Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution, 29(8), 1969-1973. doi: 10.1093/molbev/mss075
Hoelzle, K., Winkler, M., Kramer, M. M., Wittenbrink, M. M., Dieckmann, S. M., & Hoelzle, L. E. (2011). Detection of Candidatus Mycoplasma haemobos in cattle with anaemia. Veterinary Journal, 187(3), 408-410. doi: 10.1016/j.tvjl.2010.01.016
Ikeda, P., Seki, M., Carrasco, A., Rudiak, L., Miranda, J., Gonçalves, S. M. M.,... André, M. R. (2017). Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil. Epidemiology & Infection, 145(10), 2038-2052. doi: 10.1017/S0950268817000966
Katoh, K., & Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30(4), 772-780. doi: 10. 1093/molbev/mst010
Machado, C. A. L., Vidotto, O., Conrado, F. O., Santos, N. J. R., Valente, J. D. M., Barbosa, I. C.,... Vieira, R. F. C. (2017). Mycoplasma ovis infection in goat farms from northeastern Brazil. Comparative Immunology, Microbiology and Infectious Diseases, 55(1), 1-5. doi: 10.1016/j.cimid.2017.08.004
Mascarelli, P. E., Keel, M. K., Yabsley, M., Last, L. A., Breitschwerdt, E. B., & Maggi, R. G. (2014). Hemotropic mycoplasmas in little brown bats (Myotis lucifugus). Parasites & Vectors, 7(1), 117. doi: 10.1186/1756-3305-7-117
Millán, J., Di Cataldo, S., Volokhov, D. V., & Becker, D. J. (2020). Worldwide occurrence of haemoplasmas in wildlife: Insights into the patterns of infection, transmission, pathology and zoonotic potential. Transboundary and Emerging Diseases, 00, 1-21. doi: 10.1111/tbed.13932
Millán, J., López-Roig, M., Delicado, V., Serra-Cobo, J., & Esperón, F. (2015). Widespread infection with hemotropic mycoplasmas in bats in Spain, including a hemoplasma closely related to “Candidatus Mycoplasma hemohominis”. Comparative Immunology, Microbiology and Infectious Diseases, 39(1), 9-12. doi: 10.1016/j.cimid.2015.01.002
Mongruel, A. C. B., Spanhol, V. C., Valente, J. D. M., Porto, P. P., Ogawa, L., Otomura, F.,... Vieira, R. F. C. (2020). Survey of vector-borne and nematode parasites involved in the etiology of anemic syndrome in sheep from Southern Brazil. Revista Brasileira de Parasitologia Veterinária, 29(3), e007320. doi: 10. 1590/S1984-29612020062
Reis, N. R., Peracchi, A. L., Batista, C. B., Lima, I. P. de, & Pereira, A. D. (2017). História natural dos morcegos brasileiros: chave de identificação de espécies. Rio de Janeiro: Technical Books.
Santos, L. C., Vidotto, O., Santos, N. J. R., Ribeiro, J., Pellizzaro, M., Santos, A. P.,... Biondo, A. W. (2020). Hemotropic mycoplasmas (hemoplasmas) in free-ranging bats from Southern Brazil. Comparative Immunology, Microbiology and Infectious Diseases, 69(1), 101416. doi: 10.1016/j.cimid.2020.101416
Schipper, J., Chanson, J. S., Chiozza, F., Cox, N. A., Hoffmann, M., Katariya, V.,… Young, B. E. (2008). The status of the world’s land and marine mammals: diversity, threat, and knowledge. Science, 322(5899), 255-230. doi: 10.1126/science.1165115
Sela, I., Ashkenazy, H., Katoh, K., & Pupko, T. (2015). Guidance 2: accurate detection of unreliable alignment regions accounting for the uncertainty of multiple parameters. Nucleic Acids Research, 43(1), 7-14. doi: 10.1093/nar/gkv318
Volokhov, D. V., Becker, D. J., Bergner, L. M., Camus, M. S., Orton, R. J., Chizhikov, V. E.,… Streicker, D. G. (2017). Novel hemotropic mycoplasmas are widespread and genetically diverse in vampire bats. Epidemiology & Infection, 145(15), 3154-3167. doi: 10.1017/S095026881700231X
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2022-01-10
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Collere, F. C. M., Ferrari, L. D. R., Drozino, R. N., Valente, J. D. M., Massini, P. F., Otomura, F. H., Toledo, M. J. de O., Vieira, T. S. W. J., & Vieira, R. F. da C. (2022). Hemotropic mycoplasmas in bats from forest fragments, state of Paraná, southern Brazil. Semina: Ciências Agrárias, 43(1), 431–440. https://doi.org/10.5433/1679-0359.2022v43n1p431
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