Cotesia flavipes Cameron (Hymenoptera: Braconidae) alters the nutrients in the hemolymph, fat body, and cytochemistry of Diatraea flavipennella Box (Lepidoptera: Crambidae) hemocytes

Eliana Maria dos Passos; Valéria Wanderley-Teixeira; Ana Lucia Figueiredo Porto; Edmilson Jacinto Marques; Álvaro Aguiar Coelho Teixeira; Flávio Silva Oliveira Silva

doi:10.5433/1679-0359.2019v40n2p539

Autores/as

Eliana Maria dos Passos Empresa de Desenvolvimento Agropecuário de Sergipe, EMDAGRO http://orcid.org/0000-0002-2361-7674
Valéria Wanderley-Teixeira Universidade Federal Rural de Pernambuco http://orcid.org/0000-0001-9533-5476
Ana Lucia Figueiredo Porto Universidade Federal Rural de Pernambuco http://orcid.org/0000-0001-5561-5158
Edmilson Jacinto Marques Universidade Federal Rural de Pernambuco http://orcid.org/0000-0002-4836-2911
Álvaro Aguiar Coelho Teixeira Universidade Federal Rural de Pernambuco http://orcid.org/0000-0001-5940-9220
Flávio Silva Oliveira Silva Universidade Federal Rural de Pernambuco http://orcid.org/0000-0003-0639-2132

DOI:

https://doi.org/10.5433/1679-0359.2019v40n2p539

Palabras clave:

Sugarcane borer, Fat body, Hemocyte, Hemolymph, Parasitoid.

Resumen

Nutritional and physiological interactions depend on the evolutionary history of the host-parasitoid interaction considering that the host’s regulation by the parasitoid is species-specific. Cotesia flavipes Cameron was introduced in Brazil in the 70’s to be used in the control of Diatraea sugarcane borer complex; however, Diatraea flavipennella Box is restricted to Brazil. Thus, this study investigated the possible nutritional changes in the hemolymph and fat body, and cytochemistry of D. flavipennella hemocytes parasitized by Cotesia flavipes. The levels of nutrients were quantified using colorimetric tests in the hemolymph and fat body. Levels of acidic polysaccharides, mucin, and lipids were investigated only in hemocytes using cytochemical techniques. In addition, histological sections of parasitized and non-parasitized caterpillars were prepared to observe volume changes and the presence of glycogen in the fat body. The results showed a reduction in protein and lipid concentration in the hemolymph of parasitized caterpillars. The parasitism reduced the levels of total carbohydrate, glycogen, and protein in the fat body and almost completely consumed the perivisceral fat body. It changed the cytochemistry of hemocytes and reduced acidic glycans in prohemocytes and oenocytoids, mucin in plasmatocytes and granulocytes, and lipids in adipohemocytes, granulocytes, and prohemocytes. We concluded that the presence of the parasitoid leads to different changes in D. flavipennella hemolymph and fat body and alters the cytochemistry of hemocytes causing a significant reduction in the reserve tissue. The results from this study contribute to consolidating the use of C. flavipes in biological control programs targeting D. flavipennella in sugarcane crops.

Biografía del autor/a

Eliana Maria dos Passos, Empresa de Desenvolvimento Agropecuário de Sergipe, EMDAGRO

Pesquisador, Empresa de Desenvolvimento Agropecuário de Sergipe, EMDAGRO, Aracaju, SE, Brasil.

Valéria Wanderley-Teixeira, Universidade Federal Rural de Pernambuco

Profa, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, UFRPE, Recife, PE, Brasil.

Ana Lucia Figueiredo Porto, Universidade Federal Rural de Pernambuco

Profa, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, UFRPE, Recife, PE, Brasil.

Edmilson Jacinto Marques, Universidade Federal Rural de Pernambuco

Prof., Departamento de Agronomia, UFRPE, Recife, PE, Brasil

Álvaro Aguiar Coelho Teixeira, Universidade Federal Rural de Pernambuco

Prof., Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, UFRPE, Recife, PE, Brasil.

Flávio Silva Oliveira Silva, Universidade Federal Rural de Pernambuco

Discente, Departamento de Morfologia e Fisiologia Animal, UFRPE, Recife, PE, Brasil.

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