Ceftiofur efficacy in the intramammary extended treatment of staphylococcal subclinical mastitis in primiparous cows
DOI:
https://doi.org/10.5433/1679-0359.2022v43n5p1957Keywords:
Cefalosporin, Staphylococcus aureus, Mammary, Mastitis, Cows, Extended therapy.Abstract
Extended therapy is an alternative approach to treat nonresponsive staphylococcal mastitis, although has been used mainly in adult cows. This study investigated the efficacy of intramammary ceftiofur in the treatment of staphylococcal subclinical mastitis in heifers. Sixty heifers Holstein were divided into four groups (G1 = Group negative to staphylococci infection, G2 = positive to staphylococci infection untreated Group, G3 = Group treated early lactation, G4 = treated late lactation group) with milk samples collected at five moments (M0 = diagnosis of subclinical mastitis, M1 = 7 days after diagnosis of subclinical mastitis and early extended therapy, M2 = 14 after the diagnosis of subclinical mastitis, M3 = 21 days after diagnosis of mastitis) and M4 = 28 days after diagnosis of mastitis). In the two groups G3 and G4, treated with intramammary ceftiofur, the microbiological cure was evidenced by a reduction in SCC 73.3% and 46.7%, respectively. Thus, in the treated groups (G3 + G4) and untreated (G2), cure rates were respectively 60.00% and 26.66% (P <0.05). Isolated 45 strains of Staphylococcus spp, e.g., S. aureus (51.1%) and S. intermedius (48.8%). The in vitro antimicrobial susceptibility profile showed higher sensitivity indices of isolates for oxacillin (80%), ceftiofur (77.8%), cephalexin (77.8%), ciprofloxacin (66.7%), and gentamicin (60%). In contrast, cloxacillin (24.4%), penicillin (35.6%), and tetracycline (44.5%) were the least effective antimicrobials for staphylococci isolates. Among the milk samples taken when M0 and M1 (7 days after isolation), 13.3% were positive for the detection of the inhibiting substance in Delvotest™ and all negative in the Snap test™, whereas, in the M2 (14 days after isolation), 55% were positive in Delvotest™ and 46.7% in the Snap test™. Twenty-one days after diagnosis (M3), 16.66% revealed positive results in Delvotest™ and 11.66% in the Snap test™. For the M4 (28 days after isolation), 11.66% showed positive results in Delvotest ™ and 8.33% in the Snap test™. There was a reduction in SCC among all healed animals as G2 in M0, M4 for G3 in M0, M4, and G4 in M0, M4, with p<0.05. Significant results were identified for milk production in the MO, so G1 produced more milk compared to the other three groups G2, G3, and G4. Extended intramammary therapy with ceftiofur in bovine primiparous subclinically affected by staphylococcal mastitis is effective mainly in cases treated at the beginning of lactation, showing microbiological cure and reduction in SCC.
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Campos, B., Pickering, A. C., Rocha, L. S., Aguilar, A. P., Fabres-Klein, M. H., Oliveira Mendes, T. A. de, Fitzgerald, J. R., & Oliveira Barros Ribon, A. de. (2022). Diversity and pathogenesis of Staphylococcus aureus from bovine mastitis: current understanding and future perspectives. BMC Veterinary Research, 18(1), 115. doi: 10.1186/s12917-022-03197-5122112121
Cheng, W. N., & Han, S. G. (2020). Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments - a review. Asian-Australasian Journal of Animal Sciences, 33(11), 1699-1713. doi: 10.5713/ ajas.20.0156
Clinical and Laboratory Standards Institute (2020). Performance standards of Antimicrobial Susceptibility Testing. (30th ed). Wayne, PA.
Constable, P. D., Hinchliff, K. W., Done, S., & Gruenberg, W. (2016). Veterinary medicine: a textbook of the diseases of cattle, horses, sheep, pigs, and goats (11nd ed.). Saunders Ltd.
Daniel, W. W. (2009). Biostatistics: a foundation for analysis in the health sciences (9nd ed.). Wiley.
Field, A. (2012). Discovering statistics using IBM SPSS Statistics (4nd ed.). Sage Publications Limited Textbooks.
Giguère, S., Prescott, J. F., & Dowling, P. M. (2013). Antimicrobial therapy in veterinary medicine (5nd ed.). Wiley Blackwell.
Kasravi, R., Bolourchi, M., Farzaneh, N., Seifi, H. A., Barin, A., Hovareshti, P., & Gharagozlou, F. (2011). Efficacy of conventional and extended intramammary treatment of persistent subclinical mastitis with cefquinome in lactating dairy cows. Tropical Animal Health and Production, 43(6), 1203-1210. doi: 10. 1007/s11250-011-9826-0
Langoni, H., Salina, A., Oliveira, G. C., Junqueira, N. B., Menozzi, B. D., & Joaquim, S. F. (2017). Considerations on the treatment of mastitis. Pesquisa Veterinária Brasileira, 37(11), 1261-1269. doi: 10. 1590/S0100-736X2017001100011
Magiorakos, A. P., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske, C. G., Harbarth, S., Hindler, J. F., Kahlmeter, G., Liljequist, B. O., Paterson, D. L., Rice, L. B., Stelling, J., Struelens, M. J., Vatopoulos, A., Weber, J. T., & Monnet, D. L. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3), 268-281. doi: 10.1111/j.1469-0691.2011.03570.x
McDougall, S., Clausen, L. M., Hussein, H. M., & Compton, C. W. R. (2022). Therapy of subclinical mastitis during lactation. Antibiotics, 11(2), 209. doi: 10.3390/antibiotics11020209
McDougall, S., Clausen, L., Hintukainen, J., & Hunnam, J. (2019). Randomized, controlled, superiority study of extended duration of therapy with an intramammary antibiotic for treatment of clinical mastitis. Journal Dairy Science, 102(5), 4376-4386. doi: 10.3168/jds.2018-15141
McParland, S., Dillon, P. G., Flynn, J., Ryan, N., Arkins, S., & Kennedy, A. (2019). Effect of using internal teat sealant with or without antibiotic therapy at dry-off on subsequent somatic cell count and milk production. Journal Dairy Science, 102(5), 4464-4475. doi: 10.3168/jds.2018-15195
Middleton, J. R., Timms, L. L., Bader, G. R., Lakritz, J., Luby, C. D., & Steevens, B. J. (2005). Effect of prepartum intramammary treatment with pirlimycin hydrochloride on prevalence of early first-lactation mastitis in dairy heifers. Journal of the American Veterinary Medical Association, 227(12), 1969-1974. doi: 10.2460/javma.2005.227.1969
National Mastitis Council (2006). Dry Cow Therapy. https://nmconline.org/drycow.htm
Nitz, J., Krömker, V., Klocke, D., Wente, N., Zhang, Y., & Tho Seeth, M. (2020). Intramammary infections in heifers-time of onset and associated risk factors. Animals, 10(6), 1053. doi: 10.3390/ani10061053
Oliver, S. P., Gillespie, B. E., Headrick, S. J., Moorehead, H., Lunn, P., Dowlen, H. H., Johnson, D. L., Lamar, K. C., Chester, S. T., & Moseley, W. M. (2004). Efficacy of extended ceftiofur intramammary therapy for treatment of subclinical mastitis in lactating dairy cows. Journal Dairy Science, 87(8), 2393-400. doi: 10.3168/jds.S0022-0302(04)73361-5
Quinn, P. J., Markey, B. K., Leonard, F. C., Fitzpatrick, E. S., Fanning, S., & Hartigan, P. J. (2011). Veterinary microbiology and microbial disease (2nd ed.). Wiley-Blackwell.
Ribeiro, M. G., Risseti, R. M., Bolaños, C. A., Caffaro, K. A., Morais, A. C. de, Lara, G. H., Zamprogna, T. O., Paes, A. C., Listoni, F. J., & Franco, M. M. (2015). Trueperella pyogenes multispecies infections in domestic animals: a retrospective study of 144 cases (2002 to 2012). Veterinary Quarterly, 35(2), 82-87. doi: 10.1080/01652176.2015.1022667
Roy, J. P., DesCôteaux, L., DuTremblay, D., Beaudry, F., & Elsener, J. (2009). Efficacy of a 5-day extended therapy program during lactation with cephapirin sodium in dairy cows chronically infected with Staphylococcus aureus. The Canadian Veterinary Journal, 50(12), 1257-1262.
Sachi, S., Ferdous, J., Sikder, M. H., & Azizul Karim Hussani, S. M. (2019). Antibiotic residues in milk: past, present, and future. Journal of Advanced Veterinary and Animal Research, 6(3), 315-332. doi: 10.5455/ javar.2019.f350
Sharun, K., Dhama, K., Tiwari, R., Gugjoo, M. B., Iqbal Yatoo, M., Patel, S. K., Pathak, M., Karthik, K., Khurana, S. K., Singh, R., Puvvala, B., Amarpal, Singh, R., Singh, K. P., & Chaicumpa, W. (2021). Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review. The Veterinary Quarterly, 41(1), 107-136. doi: 10.1080/01652176.2021.1882713
Sol, J., Sampimon, O. C., Barkema, H. W., & Schukken, Y. H. (2000). Factors associated with cure after therapy of clinical mastitis caused by Staphylococcus aureus. Journal Dairy Science, 83(2), 278-284. doi: 10.3168/jds.S0022-0302(00)74875-2
Swinkels, J. M, Cox, P., Schukken, Y. H., Lam, T. J. (2013). Efficacy of extended cefquinome treatment of clinical Staphylococcus aureus mastitis. Journal Dairy Science, 96(8), 4983-4992. doi: 10.3168/jds.2012-6197
Truchetti, G., Bouchard, E., Descôteaux, L., Scholl, D., & Roy, J. P. (2014). Efficacy of extended intramammary ceftiofur therapy against mild to moderate clinical mastitis in Holstein dairy cows: a randomized clinical trial. Canadian Journal of Veterinary Research, 78(1), 31-37.
Wald, R., Hess, C., Urbantke, V., Wittek, T., & Baumgartner, M. (2019). Characterization of Staphylococcus species isolated from bovine quarter milk samples. Animals, 9(5), 200. doi: 10.3390/ani9050200
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