Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
DOI:
https://doi.org/10.5433/1679-0359.2021v42n1p439Keywords:
Coalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilum, Nested-PCR.Abstract
The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP).References
Agência de Desenvolvimento Sustentável de Seridó (2011). Diagnóstico da bacia leiteira do território Seridó. Caicó-RN: Colegiado territorial do Seridó. Recuperado de http://adeseserido.blogspot.com/2012/08/ adese-disponibiliza-download-do.html
Araújo, C. P., Osório, A. L. A. R., Jorge, K. S. G., Ramos, C. A. N., Souza, A. F., Fº., Vidal, C. E. S.,... Araújo, F. R. (2014). Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1’. PLoS ONE, 9(3), 6. doi: 10.1371/journal.pone.0091023 Retrieved from https://www.ncbi.nlm. nih.gov/pubmed/24618787
De Medici, D., Croci, G., Delibato, E., Di Pasquale, S., Filetici, E., & Toti, L. (2003). Evaluation of DNA extraction methods for use in combination with SYBR green i real-time PCR to detect Salmonella enterica serotype Enteritidis in poultry. Applied and Environmental Microbiology, 69(6), 3456-3461. doi: 10.11 28/AEM.69.6.3456-3461.2003 Retrieved from https://aem.asm.org/content/69/6/3456
Falkinham, J. O. (2013). Ecology of nontuberculous mycobacteria where do human infections come from? Seminars in Respiratory and Critical Care Medicine, 34(1), 95-102. doi: 10.1055/s-0033-1333568 Retrieved from https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-0033-1333568
Nouioui, I., Sangal, V., Carro, L., Teramoto, K., Jando, M., Montero-Calasanz, M. D. C., Klenk, H. P. (2017). Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov. International Journal of Systematic and Evolutionary Microbiology, 67(12), 4848-4955. doi: 10.1099/ijsem.0.002350 Retrieved from https://www.microbiologyresearch.org/ content/journal/ijsem/10.1099/ijsem.0.002350#tab2
Nunes-Costa, D., Alarico, S., Pretti Dalcolmo, M., Correia-Neves, M., & Empadinhas, N. (2016). The looming tide of nontuberculous mycobacterial infections in Portugal and Brazil. Tuberculosis, 96(1), 107-119. doi: 10.1016/j.tube.2015.09.006 Retrieved from https://www.sciencedirect.com/science/article/abs/pii/S1472 979215300627
Rocha, B. B. (2013). Ocorrência de Mycobacteirum bovis em queijos coalho artesanais e fatores associados ao consumo de leite e derivados lácteos informais. Dissertação de mestrado, Universidade Federal de Viçosa, Viçosa, MG, Brasil. Recuperado de http://locus.ufv.br/handle/123456789/5161
Sarti, E. C. F. B., Rodrigues, R. A., Duarte, L. F. C., Bacanelli, G. M., Lilenbaum, W., & Araújo F. R. (2018). Detection of potentially pathogenic non-tuberculous mycobacteria in artisanal coalho cheese from the State of Paraiba, Northeast Brazil. Mycobacterial Diseases, 8(3), 1000266. doi: 10.4172/2161-1068.1000266 Retrieved from https://www.longdom.org/open-access/detection-of-potentially-pathogenic-nontuberculous-mycobacteria-inartisanal-coalho-cheese-from-the-state-of-paraiba-northeast-braz-2161-1068-1000266.pdf
Telenti, A. Marchesi, F., Balz, M., Bally, F., Böttger, C. E., & Bodmer T. (1993). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. Journal of Clinical Microbiology, 31(2), 175-178. doi: 0095-1137/93/020175-04$02.00/0 Retrieved from https:// jcm.asm.org/content/31/2/175.short
Thoen, C. O., Himes, E. M., Jarnagin, J. L., & Harrington, R., Jr. (1979). Comparison of four culture media for isolation of Mycobacterium avium complex from porcine tissues. Journal of Clinical Microbiology, 9(2), 194-196. doi: 0095-1137/79/02-0194/03$02.00/0 Retrieved from https://jcm.asm.org/content/jcm/9/ 2/194.full.pdf
Tortoli, E. (2014). Microbiological features and clinical relevance of new species of the genus Mycobacterium. Clinical Microbiology Reviews, 27(4), 727-752. doi: 10.1128/CMR.00035-14 Retrieved from https:// pubmed.ncbi.nlm.nih.gov/25278573/
Araújo, C. P., Osório, A. L. A. R., Jorge, K. S. G., Ramos, C. A. N., Souza, A. F., Fº., Vidal, C. E. S.,... Araújo, F. R. (2014). Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1’. PLoS ONE, 9(3), 6. doi: 10.1371/journal.pone.0091023 Retrieved from https://www.ncbi.nlm. nih.gov/pubmed/24618787
De Medici, D., Croci, G., Delibato, E., Di Pasquale, S., Filetici, E., & Toti, L. (2003). Evaluation of DNA extraction methods for use in combination with SYBR green i real-time PCR to detect Salmonella enterica serotype Enteritidis in poultry. Applied and Environmental Microbiology, 69(6), 3456-3461. doi: 10.11 28/AEM.69.6.3456-3461.2003 Retrieved from https://aem.asm.org/content/69/6/3456
Falkinham, J. O. (2013). Ecology of nontuberculous mycobacteria where do human infections come from? Seminars in Respiratory and Critical Care Medicine, 34(1), 95-102. doi: 10.1055/s-0033-1333568 Retrieved from https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-0033-1333568
Nouioui, I., Sangal, V., Carro, L., Teramoto, K., Jando, M., Montero-Calasanz, M. D. C., Klenk, H. P. (2017). Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov. International Journal of Systematic and Evolutionary Microbiology, 67(12), 4848-4955. doi: 10.1099/ijsem.0.002350 Retrieved from https://www.microbiologyresearch.org/ content/journal/ijsem/10.1099/ijsem.0.002350#tab2
Nunes-Costa, D., Alarico, S., Pretti Dalcolmo, M., Correia-Neves, M., & Empadinhas, N. (2016). The looming tide of nontuberculous mycobacterial infections in Portugal and Brazil. Tuberculosis, 96(1), 107-119. doi: 10.1016/j.tube.2015.09.006 Retrieved from https://www.sciencedirect.com/science/article/abs/pii/S1472 979215300627
Rocha, B. B. (2013). Ocorrência de Mycobacteirum bovis em queijos coalho artesanais e fatores associados ao consumo de leite e derivados lácteos informais. Dissertação de mestrado, Universidade Federal de Viçosa, Viçosa, MG, Brasil. Recuperado de http://locus.ufv.br/handle/123456789/5161
Sarti, E. C. F. B., Rodrigues, R. A., Duarte, L. F. C., Bacanelli, G. M., Lilenbaum, W., & Araújo F. R. (2018). Detection of potentially pathogenic non-tuberculous mycobacteria in artisanal coalho cheese from the State of Paraiba, Northeast Brazil. Mycobacterial Diseases, 8(3), 1000266. doi: 10.4172/2161-1068.1000266 Retrieved from https://www.longdom.org/open-access/detection-of-potentially-pathogenic-nontuberculous-mycobacteria-inartisanal-coalho-cheese-from-the-state-of-paraiba-northeast-braz-2161-1068-1000266.pdf
Telenti, A. Marchesi, F., Balz, M., Bally, F., Böttger, C. E., & Bodmer T. (1993). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. Journal of Clinical Microbiology, 31(2), 175-178. doi: 0095-1137/93/020175-04$02.00/0 Retrieved from https:// jcm.asm.org/content/31/2/175.short
Thoen, C. O., Himes, E. M., Jarnagin, J. L., & Harrington, R., Jr. (1979). Comparison of four culture media for isolation of Mycobacterium avium complex from porcine tissues. Journal of Clinical Microbiology, 9(2), 194-196. doi: 0095-1137/79/02-0194/03$02.00/0 Retrieved from https://jcm.asm.org/content/jcm/9/ 2/194.full.pdf
Tortoli, E. (2014). Microbiological features and clinical relevance of new species of the genus Mycobacterium. Clinical Microbiology Reviews, 27(4), 727-752. doi: 10.1128/CMR.00035-14 Retrieved from https:// pubmed.ncbi.nlm.nih.gov/25278573/
Downloads
Published
2021-01-19
How to Cite
Medeiros, G. B., Rodrigues, O. G., Bezerra Neto, P. S., Medeiros, R. S. de, Sarti, E. C. F. B., Araújo, F. R. de, … Higino, S. S. dos S. (2021). Detection of mycobacteria in coalho cheese sold in Northeastern Brazil. Semina: Ciências Agrárias, 42(1), 439–446. https://doi.org/10.5433/1679-0359.2021v42n1p439
Issue
Section
Communication
License
Copyright (c) 2020 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
O Copyright dos manuscritos publicados pertence ao periódico. Como são publicados em um periódico de acesso aberto, eles estão disponíveis gratuitamente, para uso privado ou para fins educacionais e não comerciais.
A revista tem o direito de fazer, no documento original, alterações referentes às normas lingüísticas, ortografia e gramática, com o objetivo de garantir as normas padrão do idioma e a credibilidade da revista. No entanto, respeitará o estilo de escrita dos autores.
Quando necessário, alterações conceituais, correções ou sugestões serão encaminhadas aos autores. Nesses casos, o manuscrito deve ser submetido a uma nova avaliação após revisão.
A responsabilidade pelas opiniões expressas nos manuscritos é inteiramente dos autores.
