Corn silage aerobic stability, dry matter loss, and microbiology are affected by the use of organic acids, depending on the plant maturity stage
DOI:
https://doi.org/10.5433/1679-0359.2025v46n5p1629Keywords:
Aerobic stability, Ammonium hydroxide, Corn silage, Dry matter loss, Propionic acid, Silage additive.Abstract
This study aimed to evaluate the fermentation profile, nutritional quality, microbial count, fermentation losses, and aerobic stability of corn silages at different stages of maturity that were treated with organic acids (OA; Mold-Zap, Alltech, Nicholasville, KY). The OA (8 g kg-1 DM) was applied to corn silage harvested at the early, medium, or late stage, before compaction in the bunk. Lactic acid decreased with silage maturity (P<0.01). Compared to the control, the OA-treated silage had higher lactic acid content in the early-maturity silage (90.0 vs. 51.7 g kg-1 DM), but lower lactic acid in [1]the late-maturity silage (33.7 vs. 51.6 g kg-1 DM). Both early- and late-maturity silages with OA had lower yeast counts and DM losses (P<0.01). Silage heating upon air exposure was also lower in late-maturity silage with OA (148 vs. 112 hours of aerobic stability). The use of OA increased the soluble fraction (a), the potentially degradable fraction (b), and the effective degradability (ED) (P<0.01), and reduced the undegradable fraction (U) in intermediate- (268 vs. 287 g kg-1 DM) and late-maturity (245 vs. 322 g kg-1 DM) silages. In general, organic acids effectively mitigated aerobic deterioration and promoted DM digestibility in corn silage with a DM content above 350 g kg-1.
Downloads
References
Association of Official Analytical Chemists (1995). Official Methods of Analysis. (15nd ed.)
Auerbach, H., & Nadeau, E. (2018). Biological and chemical additives maintain nutritive value of grass silage during air exposure. In K. Gerlach, & K.-H. Südekum (Eds.), Proceeding of the Internationa Silage Conference, Bonn, Germany, 18.
Auerbach, H., Nadeau, E., Weiss, K., & Theobald, P. (2016). Effects of sodium nitrite-containing additives on dry matter losses, fermentation pattern and biogenic amine formation in lucerne and cocksfoot silage. Proceedings of the International Conference on Forage Conservation, Horny Smokovec, Slovak Republic, 17.
Bernardi, A., Härter, C. J., Silva, A. W., Reis, R. A., & Rabelo, C. H. (2019). A meta-analysis examining lactic acid bacteria inoculants for maize silage: effects on fermentation, aerobic stability, nutritive value, and livestock production. Grass Forage Science, 74(4), 596-612. doi: 10.1111/gfs.12452 DOI: https://doi.org/10.1111/gfs.12452
Borreani, G., Tabacco, E., Schmidt, R. J., Holmes, B. J., & Muck, R. E. (2018). Silage review: factors affecting dry matter and quality losses in silages. Journal of Dairy Science, 101(5), 3952-3979. doi: 10.3168/jds.2017-13837 DOI: https://doi.org/10.3168/jds.2017-13837
Bueno, J. L., Bolson, D. C., Jacovaci, F. A., Gomes, A. L. M., Ribeiro, M. G., Bueno, A. V. I., Jobim, C. C., & Daniel, J. L. P. (2020). Storage length interacts with maturity to affect nutrient availability in unprocessed flint corn silage. Revista Brasileira Zootecnia, 49, e20190247. doi: 10.37496/rbz4920190247 DOI: https://doi.org/10.37496/rbz4920190247
Cherney, J. H., & Cherney, D. J. R. (2003). Assessing silage quality. In L. J. Buxton, R. E. Muck, & J. H. Harrison (Eds.), Silage science and technology (pp. 141-198). American Society of Agronomy. DOI: https://doi.org/10.2134/agronmonogr42.c4
Danner, H., Holzer, M., Mayrhuber, E., & Braun, R. (2003). Acetic acid increases stability of silage under aerobic conditions. Applied Environment Microbiology, 69(1), 562-567. doi: 10.3168/jds.S0022-0302(93)77414-7 DOI: https://doi.org/10.1128/AEM.69.1.562-567.2003
Dewar, W. A., McDonald, P., & Whittenbury, R. (1963). The hydrolysis of grass hemicelluloses during ensilage. Journal Science Food Agriculture, 14(6), 411-417. doi: 10.1002/jsfa.2740140610 DOI: https://doi.org/10.1002/jsfa.2740140610
Ferraretto, L. F., & Shaver, R. D. (2012). Meta-analysis: effect of corn silage harvest practices on intake, digestion, and milk production by dairy cows. The Professional Animal Scientist, 28, 141-149. doi: 10.15232/s1080-7446(15)30334-x DOI: https://doi.org/10.15232/S1080-7446(15)30334-X
Filya, I. 2004. Nutritive value and aerobic stability of whole crop maize silage harvested at four stages of maturity. Animal Feed Science Technology, 116(1-2), 141-150. doi: 10.1016/j.anifeedsci.2004.06.003 DOI: https://doi.org/10.1016/j.anifeedsci.2004.06.003
Goering, H. K., & Van Soest, P. (1970). Forage fiber analyses (apparatus, reagents, procedures, and some applications). (Agric. Handbook, 379). ARS-USDA.
Halverson, A. W., & Emerick, R. J. (1982). Nitrogen distribution and acid production in corn silage treated with ammonium hydroxide. Journal of Agricultural and Food Chemistry, 30(3), 474-477. doi: 10.1021/jf00111a016 DOI: https://doi.org/10.1021/jf00111a016
Hoffman, P. C., Esser, N. M., Shaver, R. D., Coblentz, W. K., Scott, M. P., Bodnar, A. L., Schmidt, R. J., & Charley, R. C. (2011). Influence of ensiling time and inoculation on alteration of the starch-protein matrix in high-moisture corn. Journal of Dairy Science, 94(5), 2465-2474. doi: 10.3168/jds.2010-3562 DOI: https://doi.org/10.3168/jds.2010-3562
Hunt, C. W., Kezar, W., & Vinande, R. (1989). Yield, chemical composition and ruminal fermentability of corn whole plant, ear, and stover as affected by maturity. Journal of Production Agriculture, 2(4), 357-361. doi: 10.2134/jpa1989.0357. DOI: https://doi.org/10.2134/jpa1989.0357
Jung, H. G., Buxton, D. R., Hatfield, R. D., & Ralph, J. (1993). Forage cell wall structure and digestibility. American Society of Agronomy. DOI: https://doi.org/10.2134/1993.foragecellwall
Knický, M., & Spörndly, R. (2009). Sodium benzoate, potassium sorbate and sodium nitrite as silage additives. Journal of the Science of Food and Agriculture, 89(15), 2659-2667. doi: 10.1002/jsfa.3771 DOI: https://doi.org/10.1002/jsfa.3771
Knický, M., & Spörndly, R. (2011). The ensiling capability of a mixture of sodium benzoate, potassium sorbate, and sodium nitrite. Journal of Dairy Science, 94(2), 824-831. doi: 10.3168/jds.2010-3364 DOI: https://doi.org/10.3168/jds.2010-3364
Knický, M., & Spörndly, R. (2015). Use of a mixture of sodium nitrite, sodium benzoate, and potassium sorbate in aerobically challenged silages. Journal of Dairy Science, 98(8), 5729-5734. doi: 10.3168/jds.2015-9332 DOI: https://doi.org/10.3168/jds.2015-9332
König, W., Lamminen, M., Weiss, K., Tuomivirta, T. T., Sanz Muñoz, S., Fritze, H., Elo, K., Puhakka, L., Vanhatalo, A., & Jaakkola, S. (2017). The effect of additives on the quality of white lupin-wheat silages passed by fermentation pattern and qPCR quantification of clostridia. Grass and Forage Science, 72(4), 757-777. doi: 10.1111/gfs.12276 DOI: https://doi.org/10.1111/gfs.12276
Kung, L., Jr., & Ranjit, N. K. (2001). The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silages. Journal of Dairy Science, 84(5), 1149-1155. doi: 10.3168/jds.S0022-0302(01)74575-4 DOI: https://doi.org/10.3168/jds.S0022-0302(01)74575-4
Kung, L., Jr., Myers, C. L., Neylon, J. M., Taylor, C. C., Lazartic, J., Mills, J. A., & Whiter, A. G. (2004). The effects of buffered propionic acid-based additives alone or combined with microbial inoculation on the fermentation of high moisture corn and whole-crop barley. Journal of Dairy Science, 87(5), 1310-1316. doi: 10.3168/jds.S0022-0302(04)73280-4 DOI: https://doi.org/10.3168/jds.S0022-0302(04)73280-4
Kung, L., Jr., Robinson, J. R., Ranjit, N. K., Chen, J. H., Golt, C. M., & Pesek, J. D. (2000). Microbial populations, fermentation end-products, and aerobic stability of corn silage treated with ammonia or a propionic acid-based preservative. Journal of Dairy Science, 83(7), 1479-1486. doi: 10.3168/jds.S0022-0302(00)75020-X DOI: https://doi.org/10.3168/jds.S0022-0302(00)75020-X
Kung, L., Jr., Shaver, R. D., Grant, R. J., & Schmidt, R. J. (2018). Silage review: interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science, 101(5), 4020-4033. doi: 10.3168/jds.2017-13909 DOI: https://doi.org/10.3168/jds.2017-13909
Kung, L., Jr., Sheperd, A. C., Smagala, A. M., Endres, K. M., Bessett, C. A., Ranjit, N. K., & Glancey, J. L. (1998). The effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration. Journal of Dairy Science, 81(5), 1322-1330. doi: 10.3168/jds.S0022-0302(98)75695-4 DOI: https://doi.org/10.3168/jds.S0022-0302(98)75695-4
Li, X., Hansen, W. P., Otterby, D. E., Linn, J. G., & Kuehn, C. S. (1992). Effect of additives on fermentation of corn silage containing different amounts of added nitrate nitrogen. Journal of Dairy Science, 75(6), 1555-1561. doi: 10.3168/jds.S0022-0302(92)77912-0 DOI: https://doi.org/10.3168/jds.S0022-0302(92)77912-0
McDonald, P., Henderson, A. R., & Heron, S. J. E. (1991). The biochemistry of silage (2nd ed.). Chalcombe Publications.
Muck, R. E. (2010). Silage microbiology and its control through additives. Revista Brasileira de Zootecnia, 39, 183-191. doi: 10.1590/S1516-35982010001300021 DOI: https://doi.org/10.1590/S1516-35982010001300021
Nadeau, E. (2007). Effects of plant species, stage of maturity and additive on the feeding value of whole‐crop cereal silage. Journal of the Science of Food and Agriculture, 87(5), 789-801. doi: 10.1002/jsfa.2773 DOI: https://doi.org/10.1002/jsfa.2773
Neumann, M., Mühlbach, P. R. F., Nörnberg, J. L., Ost, P. R., Restle, J., Sandini, I. E., & Romano, M. A. (2007). Characteristics fermentative obtained of the different silos type silage a effect of particle size and cutting height of corn plant. Ciência Rural, 37(3), 847-854. doi: 10.1590/S0103-84782007000300038 DOI: https://doi.org/10.1590/S0103-84782007000300038
Neumann, M., Santos, L. C., Askel, E. J., Venancio, B. J., Pontarolo, G. B., Cristo, F. B., Plodoviski, D. C., & Silva, E. P. (2021). Ruminal kinetics and degradability of energetic feedstuffs used in diets for ruminants. Ciência Animal Brasileira, 22, e-68993. doi: 10.1590/1809-6891v22e-68993 DOI: https://doi.org/10.1590/1809-6891v22e-68993
Nocek, J. E. (1988). In situ and other methods to estimate ruminal protein and energy digestibility: a review. Journal of Dairy Science, 71(8), 2051-2069. doi: 10.3168/jds.S0022-0302(88)79781-7 DOI: https://doi.org/10.3168/jds.S0022-0302(88)79781-7
Oliveira, M. R., Bueno, A. V. I., Leão, G. F. M., Neumann, M., & Jobim, C. C. (2018). Nutritional composition and aerobic stability of wheat and corn silages stored under different environmental conditions. Semina: Ciências Agrárias, 39(1), 253-260. doi: 10.5433/1679-0359.2018v39n1p253 DOI: https://doi.org/10.5433/1679-0359.2018v39n1p253
Ørskov, E. R., & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, 92(2), 499-503. doi: 10.1017/S0021859600063048 DOI: https://doi.org/10.1017/S0021859600063048
Pahlow, G., Muck, R. E., Driehuis, F., Elferink, S. J. O., & Spoelstra, S. F. (2003). Microbiology of ensiling. In D. R. Buxton (Ed.), Silage science and technology (pp. 31-94). Madison, WI. DOI: https://doi.org/10.2134/agronmonogr42.c2
Pinto, S., Warth, J. F. G., Novinski, C. O., & Schmidt, P. (2020). Effects of natamycin and Lactobacillus buchneri on the fermentative process and aerobic stability of maize silage. Journal of Animal Feed Science, 29(1), 82-89. doi: 10.22358/jafs/118179/2020 DOI: https://doi.org/10.22358/jafs/118179/2020
Pryce, J. D. (1969). A modification of the Barker-Summerson method for the determination of lactic acid. Analyst, 94, 1151-1152. doi: 10.1039/AN9699401151 DOI: https://doi.org/10.1039/an9699401151
Rabelo, C. H. S., Rezende, A. V. D., Nogueira, D. A., Rabelo, F. H. S., Simone Silvia, S., Vieira, P. D. F., Barbosa, L. Á., & Carvalho, A. (2012). Perdas fermentativas e estabilidade aeróbia de silagens de milho inoculadas com bactérias ácido-láticas em diferentes estádios de maturidade. Revista Brasileira de Saúde e Produção Animal, 13(3), 656-668. http://www.rbspa.ufba.br/ DOI: https://doi.org/10.1590/S1519-99402012000300006
Ranjit, N. K., & Kung, L., Jr. (2000). The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. Journal of Dairy Science, 83(3), 526-535. doi: 10.3168/jds.S0022-0302(00)74912-5 DOI: https://doi.org/10.3168/jds.S0022-0302(00)74912-5
Ritchie, S. W., Hanway, J. J., & Benson, G. O. (1993). How a corn plant develops. Iowa State University of Science and Technology, Cooperative Extension Service.
Rooke, J. A., & Hatfield, R. D. (2003). Biochemistry of ensiling. In D. R. Buxton, R. E. Muck, & J. H. Harrison (Eds.), Silage science and technology (pp. 31-95). Madison. DOI: https://doi.org/10.2134/agronmonogr42.c3
Selwet, M. (2009). Effect of propionic and formic acid mixtures on the fermentation, fungi development and aerobic stability of maize silage. Polish Journal of Agronomy, 1, 37-42. https://www.iung.pl/PJA/abstr_1_7.html
Senger, C. C. D., Mühlbach, P. R. F., Sánchez, L. M. B., Netto, D. P., & Lima, L. D. D. (2005). Composição química e digestibilidade in vitro de silagens de milho com distintos teores de umidade e níveis de compactação. Ciência Rural, 35(6), 1393-1399. doi: 10.1590/S0103-84782005000600026 DOI: https://doi.org/10.1590/S0103-84782005000600026
Silva, N. C., Nascimento, C. F., Campos, V. M., Alves, M. A., Resende, F. D., Daniel, J. L. P., & Siqueira, G. R. (2019). Influence of storage length and inoculation with Lactobacillus buchneri on the fermentation, aerobic stability, and ruminal degradability of high-moisture corn and rehydrated corn grain silage. Animal Feed Science and Technology, 251, 124-133. doi: 10.1016/j.anifeedsci.2019.03.003 DOI: https://doi.org/10.1016/j.anifeedsci.2019.03.003
Sniffen, C. J., O'Connor, J. D., Van Soest, P. J., Fox, D. G., & Russell, J. B. (1992). A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. Journal of Animal Science, 70(11), 3562-3577. doi: 10.2527/1992.70113562x DOI: https://doi.org/10.2527/1992.70113562x
Taiz, L., & Zeiger, E. (2006). Plant physiology (4nd ed.). Sinauer Associates Inc.
Taylor, C. C., & Kung, L., Jr. (2002). The effect of Lactobacillus buchneri 40788 on the fermentation and aerobic stability of high moisture corn in laboratory silos. Journal of Dairy Science, 85(6), 1526-1532. doi: 10.3168/jds.S0022-0302(02)74222-7 DOI: https://doi.org/10.3168/jds.S0022-0302(02)74222-7
Thomas, T. A. (1977). An automated procedure for the determination of soluble carbohydrates in herbage. Journal of the Science of Food and Agriculture, 28(7), 639-642. doi: 10.1002/jsfa.2740280711 DOI: https://doi.org/10.1002/jsfa.2740280711
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597. doi: 10.3168/jds.S0022-0302(91)78551-2 DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Wang, H., Ning, T., Hao, W., Zheng, M., & Xu, C. (2016). Dynamics associated with prolonged ensiling and aerobic deterioration of total mixed ration silage containing whole crop corn. Asian-Australasian Journal of Animal Sciences, 29(1), 62-72. doi: 10.5713/ajas.15.0319 DOI: https://doi.org/10.5713/ajas.15.0319
Weatherburn, M. W. (1967). Urease-Berthelot Colorimetric Method for in Vitro Determination of Urea. Analytical Chemistry, 39, 971-974. DOI: https://doi.org/10.1021/ac60252a045
Wilkinson, J. M., & Davies, D. R. (2013). The aerobic stability of silage: key findings and recent developments. Grass and Forage Science, 68(1), 1-19. doi: 10.1111/j.1365-2494.2012.00891.x DOI: https://doi.org/10.1111/j.1365-2494.2012.00891.x
Zhang, J., Guo, G., Chen, L., Li, J., Yuan, X., Yu, C., Shimojo, M., & Shao, T. (2015). Effect of applying lactic acid bacteria and propionic acid on fermentation quality and aerobic stability of oats-common vetch mixed silage on the Tibetan plateau. Animal Science Journal, 86(6), 595-602. doi: 10.1111/asj.12340 DOI: https://doi.org/10.1111/asj.12340
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Thomer Durman, Antonio Vinicius Iank Bueno, Clóves Cabreira Jobim, André Martins de Souza; Egon Henrique Horst; Paulo Victor Pinheiro Cesar, Livia Alessi Ienke, Nicolli Soethe Mokochinski, João Antonio de Arruda Giacomet, Mikael Neumann

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.











