Root protrusion as a vigor test for canola seeds under different levels of water availability
DOI:
https://doi.org/10.5433/1679-0359.2025v46n4p1025Keywords:
Brassica napus L, Quality control, Primary root.Abstract
Canola is used to produce oil for human consumption, biodiesel, and as part of crop rotation systems. This study aimed to determine whether radicle protrusion can be recommended as a vigor test for canola seeds. The experiments were conducted at the Federal University of Santa Maria, using seeds from the canola hybrids Ceres IMI and Nuola 300. Water content and 1000-seed weight were evaluated and vigor and germination tests performed. Primary root protrusion was evaluated in sand at four different water-holding capacities (80%, 60%, 40% and 20%) and four assessment times (24 h, 48 h, 72 h and 96 h). The experimental design was completely randomized, in a 4 x 4 factorial scheme with four replicates, totaling 64 experimental units. Data were analyzed using analysis of variance (ANOVA), polynomial regression, mean comparison tests, and Pearson's correlation at 5% significance, in R Studio software. The radicle protrusion test is a practical and cost-effective alternative for evaluating vigor in canola seeds and should be conducted in a substrate with 60% water-holding capacity in sand, with assessments at 72 hours.
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