Nutritional characterization and ruminal degradability of corn silage stored in different silo types
DOI:
https://doi.org/10.5433/1679-0359.2025v46n5p1611Keywords:
Dry matter losses, Silage fermentation profile, Ruminal microbiota.Abstract
This study assessed the nutritional composition, ruminal degradability, physical losses, and fermentation profile of corn silage stored in either trench silos or silo bags. A randomized block design was applied, with two treatments and four replicates, each replicate consisting of one silo. Compared with trench silos, storage in silo bags was more effective at maintaining levels of non-fibrous carbohydrates (NFC) (388.2 vs. 417.7 g kg-1 DM), net energy of lactation (NEL) (1.560 vs. 1.591 Mcal kg-1 DM), total digestible nutrients (TDN) (685.9 vs. 698.4 g kg-1 DM), and relative feed value (RFV) (133 vs. 144). Conversely, corn silage stored in trench silos showed higher values for ash (27.3 vs. 29.8 g kg-1 DM), acid detergent insoluble protein (ADIP) (99.49 vs. 110.43 g kg-1 CP), neutral detergent fiber (NDF) (451.0 vs. 482.5 g kg-1 DM), hemicellulose (193.8 vs. 207.4 g kg-1 DM), and acid detergent fiber (ADF) (257.2 vs. 275.0 g kg-1 DM). Storage in silo bags also reduced dry matter losses throughout the feeding period compared to trench silos (8.20 vs. 6.10 % in the first period, 9.40 vs. 2.50 % in the second, and 9.70 vs. 2.60 % in the third).
Downloads
References
Association of Official Analytical Chemists (1995). Official methods of analysis (16nd ed.). AOAC.
Bai, J., Ding, Z., Ke, W., Xu, D. M., Wang, M., Huang, W., Zhang, Y., Liu, F., & Guo, X. (2021). Different lactic acid bacteria and their combinations regulated the fermentation process of ensiled alfalfa: ensiling characteristics, dynamics of bacterial community and their functional shifts. Microbiological Biotechnology, 14(3), 1171-1182. doi: 10.1111/1751-7915.13785 DOI: https://doi.org/10.1111/1751-7915.13785
Bartosik, R. E., Cardoso, M. L., Carpaneto, B. B., Astiz, V., Molfese, E. R., & Torre, D. A. (2024). Evolution of industrial quality parameters of wheat during storage in white and colored silo bags: a field-scale study. Agriculture, 14(6), 841. doi: 10.3390/agriculture14060841 DOI: https://doi.org/10.3390/agriculture14060841
Bartosik, R., Urcola, H., Cardoso, L., Maciel, G., & Busato, P. (2023). Silo-bag system for storage of grains, seeds and by-products: a review and research agenda. Journal of Stored Products Research, 100(102061), 1-11. doi: 10.1016/j.jspr.2022.102061 DOI: https://doi.org/10.1016/j.jspr.2022.102061
Bolsen, K. K., Ashbell, G., & Weinberg, Z. G. (1996). Silage fermentation and silage additives - review. Asian-Australasian Journal of Animal Sciences, 9(5), 483-494. doi: 10.5713/ajas.1996.483 DOI: https://doi.org/10.5713/ajas.1996.483
Bonfá, C. S., Castro, G. H., Villela, S. D., Santos, R. A., Evangelista, A. R., Jayme, C. G., Gonçalves, L. C., Pires, O. S., Neto, & Barbosa, J. A. S. (2015). Silagem de capim-elefante adicionada de casca de maracujá. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 67(3), 801-808. doi: 10.1590/1678-4162-7982 DOI: https://doi.org/10.1590/1678-4162-7982
Borreani, G., Tabacco, E., Schmidt, R. J., Holmes, B. J., & Muck, R. E. (2018). Silage review: factors affecting dry matter and quality losses in silages. Journal of Dairy Science, 101(5), 3952-3979. doi: 10.3168/jds.2017-13837 DOI: https://doi.org/10.3168/jds.2017-13837
Costa, N. D., Monteiro, A. L., Silva, A. L., Moraes, A. D., Giostri, A. F., Stivari, T. S., Baldissera, T. C., & Pin, E. A. (2015). Considerações sobre a degradação da fibra em forragens tropicais associada com suplementos energéticos ou nitrogenados. Archivos de Zootecnia, 64(247), 31-41. doi: 10.21071/az.v64i247.504 DOI: https://doi.org/10.21071/az.v64i247.504
Erwin, E. S., Marco, G. J., & Emery, E. M. (1961). Volatile fatty acid analyses of blood and rumen fluid by gas chromatography. Journal of Dairy Science, 44(9), 1768-1771. doi: 10.3168/jds.S0022-0302(61)89956-6 DOI: https://doi.org/10.3168/jds.S0022-0302(61)89956-6
Gandra, J. R., Del Valle, T. A., Pause, A. G., Pedrini, C. A., Oliveira, E. R., Goes, R. H., Oliveira, K. M. P., Batista, J. D. O., Giovani, A., Noia, I. Z., & Acosta, A. (2022). Whole-plant soybean ensiling with chitosan and homolactic microbial inoculant: fermentative profile, aerobic stability, and sheep intake and digestibility. Revista Brasileira de Saúde e Produção Animal, 23(e2220502022), 1-16. doi: 10.1590/s1519-99402220502022 DOI: https://doi.org/10.1590/s1519-99402220502022
Garcez, B. S., Alves, A. A., Araújo, D. L., Lacerda, M. D., Souza, L. G., & Carvalho, L. F. (2016). Degradabilidade ruminal do capim colonião (Panicum maximum Jacq. cv. Colonião) em três idades pós-rebrota. Acta Scientarum Animal Sciences, 10(2), 130-134. doi: 10.21708/avb.2016.10.2.5513 DOI: https://doi.org/10.21708/avb.2016.10.2.5513
Garcez, K. F., Schneider, C. R., Bragança, L. F., Fernandes, T., Neres, M. A., Krolow, R. H., Azevedo, E. B., & Castagnara, D. D. (2023). Adição de farelos de milho, soja e arroz melhoram a conservação e valor nutricional de silagens de Tifton 85. Brazilian Journal of Animal and Environmental Research, 6(2), 1773-1790. doi: 10.34188/bjaerv6n2-066 DOI: https://doi.org/10.34188/bjaerv6n2-066
Goering, H. K., & Van Soest, P. J. (1970). Forage fiber analysis: (apparatus, reagents, procedures, and some applications). Agricultural Research Service, United States Department of Agriculture.
Goes, R. H., Souza, K. A., Patussi, R. A., Cornelio, T. D., Oliveira, E. R., & Brabes, K. C. (2010). Degradabilidade in situ dos grãos de crambe, girassol e soja, e de seus coprodutos em ovinos. Acta Scientarum Animal Sciences, 32(3), 271-277. doi: 10.4025/actascianimsci.v32i3.7913 DOI: https://doi.org/10.4025/actascianimsci.v32i3.7913
Goeser, J. P., & Combs, D. K. (2009). An alternative method to assess 24-h ruminal in vitro neutral detergent fiber digestibility. Journal of Dairy Science, 92(8), 3833-3841. doi: 10.3168/jds.2008-1136 DOI: https://doi.org/10.3168/jds.2008-1136
Gralak, E., Faria, M. V., Possato, O., Jr., Rossi, E. S., Silva, C. A., Rizzardi, D. A., Mendes, M. C., & Neumann, M. (2014). Capacidade combinatória de híbridos de milho para caracteres agronômicos e bromatológicos da silagem. Revista Brasileira de Milho e Sorgo, 13(2), 187-200. doi: 10.18512/1980-6477/rbms.v13n2p187-200 DOI: https://doi.org/10.18512/1980-6477/rbms.v13n2p187-200
Harper, K. J., & McNeill, D. M. (2015). The role iNDF in the regulation of feed intake and the importance of its assessment in subtropical ruminant systems (The role of iNDF in the regulation of forage intake). Agriculture, 5(3), 778-790. doi: 10.3390/agriculture5030778 DOI: https://doi.org/10.3390/agriculture5030778
Kung, L., Jr., Shaver, R. D., Grant, R. J., & Schmidt, R. J. (2018). Silage review: interpretation of chemical, microbial, and organoleptic components of silages. American Dairy Science Association, 101(5), 4020-4033. doi: 10.3168/jds.2017-13909 DOI: https://doi.org/10.3168/jds.2017-13909
Larsen, S. U., Hjort-Gregersen, K., Vazifehkhoran, A. H., & Triolo, J. M. (2017). Co-ensiling of straw with sugar beet leaves increases the methane yield from straw. Bioresource Technology, 245(Pt A), 106-115. doi: 10.1016/j.biortech.2017.08.117 DOI: https://doi.org/10.1016/j.biortech.2017.08.117
Macêdo, A. J., & Santos, E. M. (2019). Princípios básicos para produção de silagem. Arquivos de Ciências Veterinárias e Zoologia da UNIPAR, 22(4), 147-156. doi: 10.25110/arqvet.v22i4.2019.6948 DOI: https://doi.org/10.25110/arqvet.v22i4.2019.6948
Marafon, F., Neumann, M., Carletto, R., Wrobel, F. D., Mendes, E. D., Spada, C. A., & Faria, M. V. (2015). Características nutricionais e perdas no processo fermentativo de silagens de milho, colhidas em diferentes estádios reprodutivos com diferentes processamentos de grãos. Semina: Ciências Agrárias, 36(2), 917-932. doi: 10.5433/1679-0359.2015v36n2p917 DOI: https://doi.org/10.5433/1679-0359.2015v36n2p917
Melo, N. N., Carvalho-Estrada, P. A., Tavares, Q. G., Pereira, L. M., Vigne, G. L. D., Rezende, D. M. L. C., & Schmidt, P. (2023). The effects of short-time delayed sealing on fermentation, aerobic stability and chemical composition on maize silages. Agronomy, 13(1), 223. doi: 10.3390/agronomy13010223 DOI: https://doi.org/10.3390/agronomy13010223
Moe, P. W., & Tyrrell, H. F. (1976). Estimating metabolizable and net energy of feeds. Proceedings of the International Symposium on Feed Composition, Animal Nutrient Requirements, and Computerization of Diets, Logan, USA, 1.
Moraes, G. S. O., Guim, A., Tabosa, J. N., Chagas, J. C. C., Almeida, M. P., & Ferreira, M. A. (2019). Cactus [Opuntia stricta (Haw.) Haw] cladodes and corn silage: how do we maximize the performance of lactating dairy cows reared in semiarid regions? Livestock Science, 221, 133-138. doi: 10.1016/j.livsci.2019.01.026 DOI: https://doi.org/10.1016/j.livsci.2019.01.026
Mousquer, C. J., Silva, M. R., Castro, W. J. R., Fernandes, G. A., Fernandes, F. F. D., Silva, A. S., Fº., Feijó, L. C., & Ferreira, V. B. (2015). Potencial de utilização de silagem de gramíneas tropicais não convencionais e cana-de-açúcar. Pubvet, Publicações em Medicina Veterinária e Zootecnia, 7(22), 1-32. doi: 10.22256/pubvet.v7n22.1622 DOI: https://doi.org/10.22256/pubvet.v7n22.1622
Negrão, F. D., Zanine, A. D., Souza, A. L., Cabral, L. D., Ferreira, D. D., & Dantas, C. C. (2016). Perdas, perfil fermentativo e composição química das silagens de capim Brachiaria decumbens com inclusão de farelo de arroz. Revista Brasileira de Saúde e Produção Animal, 17(1), 13-25. doi: 10.1590/s1519-99402016000100002 DOI: https://doi.org/10.1590/S1519-99402016000100002
Neumann, M., Baldissera, E., Ienke, L. A., Souza, A. M., Oliveira, P. E. P., & Bumbieris, V. H., Jr. (2024). Nutritional value evaluation of corn silage from different mesoregions of southern Brazil. Agriculture, 14(7), 1055. doi: 10.3390/agriculture14071055 DOI: https://doi.org/10.3390/agriculture14071055
Neumann, M., Cristo, F. B., Pontarolo, G. B., Souza, A. M., Machado, M. P., Oliveira, R. S., Gavlak, T. F., & Manchur, A. D. (2021a). Effect of sealing on the feed out face of trench silos on the performance of confined calves. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 73(3), 711-720. doi: 10.1590/1678-4162-12248 DOI: https://doi.org/10.1590/1678-4162-12248
Neumann, M., Leão, G. F., Askel, E. J., Marafon, F., Figueira, D. N., & Poczynek, M. (2017). Sealing type effect on corn silage quality in bunker silos. Ciência Rural, 47(5), 1-6. doi: 10.1590/0103-8478cr20160643 DOI: https://doi.org/10.1590/0103-8478cr20160643
Neumann, M., Santos, L. C., Askel, E. J., Venancio, B. J., Pontarolo, G. B., Cristo, F. B., Plodoviski, D. C., & Silva, E. P. (2021b). Ruminal kinetics and degradability of energetic feedstuffs used in diets for ruminants. Ciência Animal Brasileira, 22(e-68993), 1-10. doi: 10.1590/1809-6891v22e-68993 DOI: https://doi.org/10.1590/1809-6891v22e-68993
Nocek, J. E. (1988). In situ and other methods to estimate ruminal protein and energy digestibility: a review. Journal of Dairy Science, 71(8), 2051-2069. doi: 10.3168/jds.s0022-0302(88)79781-7 DOI: https://doi.org/10.3168/jds.S0022-0302(88)79781-7
Orskov, E. R., & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science, 92(2), 499-503. doi: 10.1017/s0021859600063048 DOI: https://doi.org/10.1017/S0021859600063048
Paris, W., Zamarchi, G., Pavinato, P. S., & Martin, T. N. (2015). Qualidade da silagem de aveia preta sob efeito de estádios fenológicos, tamanhos de partícula e pré-murchamento. Revista Brasileira de Saúde e Produção Animal, 16(3), 486-498. doi: 10.1590/s1519-99402015000300002 DOI: https://doi.org/10.1590/S1519-99402015000300002
Pavinato, O. S., Pauletti, V., Motta, A. C. V., & Moreira, A. (2017). Manual de adubação e calagem para o estado do paraná (2a ed.). SBCS/NEPAR.
Pereira, M. C. S., Yang, W. Z., Beauchemin, K. A., McAllister, T. A., Wood, K. M., & Penner, G. B. (2021). Effect of silage source, physically effective neutral detergent fiber, and undigested neutral detergent fiber concentrations on performance and carcass characteristics of finishing steers. Translational Animal Science, 5(1), 1-13. doi: 10.1093/tas/txaa236 DOI: https://doi.org/10.1093/tas/txaa236
Pinto, M. M. F., Gonçalves, J. S., Souza, I. T. N., Batista, N. V., Melo, V. L. L., Firmino, S. S., Pinedo, L. A., & Lima, P. O. (2019). Utilização do melão (Cucumis melo L.) na alimentação de ruminantes: uma revisão. Brasilian Journal of Development, 5(12), 31466-31481. doi: 10.34117/bjdv5n12-240 DOI: https://doi.org/10.34117/bjdv5n12-240
Ramos, M. D. N., Milessi, T. S., Candido, R. G., Mendes, A. A., & Aguiar, A. (2022). Enzymatic catalysis as a tool in biofuels production in Brazil: current status and perspectives. Energy for Sustainable Development, 68, 103-119. doi: 10.1016/j.esd.2022.03.007 DOI: https://doi.org/10.1016/j.esd.2022.03.007
Santos, L. F. X., Martins, V. S., Jr., Veloso, A. C. R., Fonseca, S. A., Braz, T. G. S., & Duarte, E. R. (2023). Caracterização microbiológica e bromatológica da silagem de capim-elefante inoculada com fungos celulolíticos em dois tipos de silos experimentais. Ensaios e Ciência, 27(1), 25-31. doi: 10.17921/1415-6938.2023v27n1p25-31 DOI: https://doi.org/10.17921/1415-6938.2023v27n1p25-31
Schmidt, P., Novinski, C. O., Junges, D., Almeida, R., & Souza, C. M. de. (2015). Concentration of mycotoxins and chemical composition of corn silage: a farm survey using infrared thermography. Journal Dairy Science, 98(9), 6609-6619. doi: 10.3168/jds.2014-8617 DOI: https://doi.org/10.3168/jds.2014-8617
Silva, D. J., & Queiroz, A. C. (2009). Análise de Alimentos, métodos químicos e biológicos (3a ed.). UFV, Universidade Federal de Viçosa.
Silva, M. R., Jobim, C. C., Neumann, M., & Osmari, M. P. (2018). Corn grain processing improves chemical composition and fermentative profile of rehydrated silage. Acta Scientarum Animal Sciences, 40(e42564), 1-6. doi: 10.4025/actascianimsci.v40i1.42564 DOI: https://doi.org/10.4025/actascianimsci.v40i1.42564
Statistical Analysis System Institute (1993). SAS/STAT user’s guide: statistics, version 6. (4nd ed., vol. 2). SAS Institute.
Taher, H. I., Urcola, H. A., Cendoya, M. G., & Bartosik, R. E. (2019). Predicting soybean losses using carbon dioxide monitoring during storage in silo bags. Journal of Stored Products Research, 82, 1-8. doi: 10.1016/j.jspr.2019.03.002 DOI: https://doi.org/10.1016/j.jspr.2019.03.002
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597. doi: 10.3168/jds.s0022-0302(91)78551-2 DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Mikael Neumann, Valéria Kalinovski, Ellen Baldissera, Dayana Rochinski da Silveira Pinto, Leonardo Huçalo, Paulo Victor Pinheiro Cesar, Luísa da Costa Venancio, Arno Passarin Filho, Caroline Bordignon da Rosa, Daniel Corrêa Plodoviski

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.











