Keywords
Epidemiological survey
Mycoplasmosis
PCR
Sequencing.
Mycoplasmosis
PCR
Sequencing.
How to Cite
Santos, D. F. dos, Almeida, A. do B. P. F. de, Cayuela, M. A. A., Freitas, V. D. de, Koiyoma, M. F. G., Dutra, V., & Sousa, V. R. F. (2019). Frequency of Mycoplasma haemocanis infection in dogs of Cuiaba, Mato Grosso, Brazil. Semina: Ciências Agrárias, 40(6Supl3), 3749–3754. https://doi.org/10.5433/1679-0359.2019v40n6Supl3p3749
Abstract
Haemotropic mycoplasmas are pleomorphic, epicellular, gram-negative bacteria, which infect red blood cells. In dogs, the acute phase of this infection may lead to haemolytic anaemia potentially precipitated by splenectomy, or by immunosuppressive drugs or diseases; however, in cases of chronic infection, the signs are inapparent. Molecular techniques and microscopic observation are the most commonly used methods to diagnose mycoplasma infection, though studies about haemotropic mycoplasma infections in dogs from Cuiabá, capital of Mato Grosso, Brazil are scarce. The objective of this study was to evaluate the frequency of mycoplasma infection, the Mycoplasma species present, and factors associated with infection in 334 dogs diagnosed using polymerase chain reaction (PCR). The frequency of infection was 5.4%, and M. haemocanis was the only species identified. No epidemiological factor investigated showed a statistically significant association with infection, including tick infestation.References
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BIONDO, A. W.; SANTOS, A. P. dos; GUIMARÃES, A. M.; VIEIRA, R. F.; VIDOTTO, O.; MACIEIRA, D. D. E. B.; ALMOSNY, N. R.; MOLENTO, M. B.; TIMENETSKY, J.; MORAIS, H. A. de; GONZÁLEZ, F. H.; MESSICK, J. B. A review of the occurrence of hemoplasmas (hemotrophic mycoplasmas) in Brazil. Revista Brasileira de Parasitologia, Jaboticabal, v. 18, n. 3, p. 1-7, 2009. DOI: 10.4322/rbpv.01803001
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QUARESMA, P. F.; MURTA, S. M.; FERREIRA, E. C.; ROCHA-LIMA, A. C.; XAVIER, A. A.; GONTIJO, C. M. Molecular diagnosis of canine visceral leishmaniasis: identiï¬cation of Leishmania species by PCR-RFLP and quantiï¬cation of parasite DNA by real-time PCR. Acta Tropica, London, v. 111, n. 3, p. 289-294, 2009. DOI: 10.1016/j.actatropica.2009.05.008
RAMOS, R.; RAMOS, C.; ARAÚJO, F.; OLIVEIRA, R.; SOUZA, I.; PIMENTEL, D.; GALINDO, M.; SANTANA, M.; ROSAS, E.; FAUSTINO, M.; ALVES, L. Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). Parasitology Research, Switzerland, v. 107, n. 5, p. 1115-1120, 2010. DOI: 10.1007/s00436-010-1979-7
SAMBROOK, J.; RUSSELL, D. W. Molecular cloning: a laboratory manual. 3th ed. Nova York: Cold Spring Harbor Laboratory, 2001. v. 1, 2100 p.
SHARIFIYAZDI, H.; ABBASZADEH HASIRI, M.; AMINI, A. H. Intravascular hemolysis associated with Candidatus Mycoplasma hematoparvum in a non-splenectomized dog in the south region of Iran. Veterinary Resarch Forum, Iran, v. 5, n. 3, p. 243-6, 2014. PMID: 25568726
SILVA, B. R. D.; GARCIA, M. V.; RODRIGUES, V. D. S.; ANDREOTTI, R.; DITTRICH, R. L. Ixodidae fauna of domestic dogs in Parana, southern Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 26, n. 3, p. 375-377, 2017. DOI: 10.1590/S1984-29612017021
SOARES, R. L.; ECHEVERRIA, J. T.; PAZZUTI, G.; CLEVELAND, H. P. K.; BABO-TERRA, V. J.; FRIOZI, E.; RAMOS, C. A. N. Occurrence of Mycoplasma haemocanis in dogs infested by ticks in Campo Grande, Mato Grosso do Sul, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 25, n. 3, p. 360-363, 2016. DOI: 10.1590/S1984-29612016043
VALLE, S. F.; MESSICK, J. B.; SANTOS, A. P. dos; KREUTZ, L. C.; DUDA, N. C.; MACHADO, G.; CORBELLINI, L. G.; BIONDO, A. W.; GONZÁLEZ, F. H. Identification, occurrence and clinical findings of canine hemoplasmas in Southern Brazil. Comparative Immunology, Microbiology and Infectious Diseases, London, v. 37, n. 4, p. 259-265, 2014. DOI: 10.1016/j.cimid.2014.08.001
WENGI, N.; WILLI, B.; BORETTI, F. S.; CATTORI, V.; RIOND, B.; MELI, M. L.; REUSCH, C. E.; LUTZ, H.; HOFMANN-LEHMANN, R. Real-time PCR-based prevalence study, infection follow-up and molecular characterization of canine hemotropic mycoplasmas. Veterinary Microbiology, London, v. 126, n. 1-3, p. 132-41, 2008. DOI: 10.1016/j.vetmic.2007.06.018
ALVES, T. B.; FAGGIONA, S. A.; SANTOSA, E. V.; ROBERTOA, P. G.; FRANÇA, S. C.; FACHINA, A. L.; MARINS, M. Real-time PCR-based study of haemotrophic mycoplasmas in dogs from Ribeirão Preto, Brazil. Archivos de Medicina Veterinária, Valdivia, v. 46, n. 2, p. 333-336, 2014. DOI: 10.4067/S0301-732X2014000200021
AQUINO, L. C.; KAMANI, J.; HARUNA, A. M.; PALUDO, G. R.; HICKS, C. A.; HELPS, C. R.; TASKER, S. Analysis of risk factors and prevalence of haemoplasma infection in dogs. Veterinary Parasitology, London, v. 221, n. 1, p. 111-117, 2016. DOI: 10.1016/j.vetpar.2016.03.014
BIONDO, A. W.; SANTOS, A. P. dos; GUIMARÃES, A. M.; VIEIRA, R. F.; VIDOTTO, O.; MACIEIRA, D. D. E. B.; ALMOSNY, N. R.; MOLENTO, M. B.; TIMENETSKY, J.; MORAIS, H. A. de; GONZÁLEZ, F. H.; MESSICK, J. B. A review of the occurrence of hemoplasmas (hemotrophic mycoplasmas) in Brazil. Revista Brasileira de Parasitologia, Jaboticabal, v. 18, n. 3, p. 1-7, 2009. DOI: 10.4322/rbpv.01803001
COMPTON, S. M.; MAGGI, R. G.; BREITSCHWERDT, E. B. Candidatus Mycoplasma haematoparvum and Mycoplasma haemocanis infections in dogs from the United States. Comparative Immunology, Microbiology and Infectious Diseases, London, v. 35, n. 6, p. 557-562, 2012. DOI: 10.4322/rbpv.01803001
CRIADO-FORNELIO, A.; MARTINEZ-MARCOS, A.; BULING-SARAÑA, A.; BARBA-CARRETERO, J. C. A. M. Presence of Mycoplasma haemofelis, Mycoplasma haemominutum and piroplasmids in cats from southern Europe: a molecular study. Veterinary Microbiology, London, v. 93, n. 4, p. 307-317, 2003. DOI: 10.1016/S0378-1135(03)00044-0
KEMMING, G. I.; MESSICK, J. B.; ENDERS, G.; BOROS, M.; LORENZ, B.; MUENZING, S.; KISCH-WEDEL, H.; MUELLER, W.; HAHMANN-MUELLER, A.; MESSMER, K.; THEIN, E. Mycoplasma haemocanis infection - a kennel disease. Comparative Medicine, New York, v. 54, n. 4, p. 404-409, 2004. PMID: 15357321
NOVACCO, M.; MELI, M. L.; GENTILINI, F.; MARSILIO, F.; CECI, C.; PENNISI, M. G.; LOMBARDO, G.; LLORET, A.; SANTOS, L.; CARRAPIÇO, T.; WILLI, B.; WOLF, G.; LUTZ, H.; HOFMANN-LEHMANN, R. Prevalence and geographical distribution of canine hemotropic mycoplasma infections in Mediterranean countries and analysis of risk factors for infection. Veterinary Microbiology, London, v. 142, n. 3-4, p. 276-284, 2010. DOI: 10.1016/j.vetmic.2009.09.069
QUARESMA, P. F.; MURTA, S. M.; FERREIRA, E. C.; ROCHA-LIMA, A. C.; XAVIER, A. A.; GONTIJO, C. M. Molecular diagnosis of canine visceral leishmaniasis: identiï¬cation of Leishmania species by PCR-RFLP and quantiï¬cation of parasite DNA by real-time PCR. Acta Tropica, London, v. 111, n. 3, p. 289-294, 2009. DOI: 10.1016/j.actatropica.2009.05.008
RAMOS, R.; RAMOS, C.; ARAÚJO, F.; OLIVEIRA, R.; SOUZA, I.; PIMENTEL, D.; GALINDO, M.; SANTANA, M.; ROSAS, E.; FAUSTINO, M.; ALVES, L. Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). Parasitology Research, Switzerland, v. 107, n. 5, p. 1115-1120, 2010. DOI: 10.1007/s00436-010-1979-7
SAMBROOK, J.; RUSSELL, D. W. Molecular cloning: a laboratory manual. 3th ed. Nova York: Cold Spring Harbor Laboratory, 2001. v. 1, 2100 p.
SHARIFIYAZDI, H.; ABBASZADEH HASIRI, M.; AMINI, A. H. Intravascular hemolysis associated with Candidatus Mycoplasma hematoparvum in a non-splenectomized dog in the south region of Iran. Veterinary Resarch Forum, Iran, v. 5, n. 3, p. 243-6, 2014. PMID: 25568726
SILVA, B. R. D.; GARCIA, M. V.; RODRIGUES, V. D. S.; ANDREOTTI, R.; DITTRICH, R. L. Ixodidae fauna of domestic dogs in Parana, southern Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 26, n. 3, p. 375-377, 2017. DOI: 10.1590/S1984-29612017021
SOARES, R. L.; ECHEVERRIA, J. T.; PAZZUTI, G.; CLEVELAND, H. P. K.; BABO-TERRA, V. J.; FRIOZI, E.; RAMOS, C. A. N. Occurrence of Mycoplasma haemocanis in dogs infested by ticks in Campo Grande, Mato Grosso do Sul, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 25, n. 3, p. 360-363, 2016. DOI: 10.1590/S1984-29612016043
VALLE, S. F.; MESSICK, J. B.; SANTOS, A. P. dos; KREUTZ, L. C.; DUDA, N. C.; MACHADO, G.; CORBELLINI, L. G.; BIONDO, A. W.; GONZÁLEZ, F. H. Identification, occurrence and clinical findings of canine hemoplasmas in Southern Brazil. Comparative Immunology, Microbiology and Infectious Diseases, London, v. 37, n. 4, p. 259-265, 2014. DOI: 10.1016/j.cimid.2014.08.001
WENGI, N.; WILLI, B.; BORETTI, F. S.; CATTORI, V.; RIOND, B.; MELI, M. L.; REUSCH, C. E.; LUTZ, H.; HOFMANN-LEHMANN, R. Real-time PCR-based prevalence study, infection follow-up and molecular characterization of canine hemotropic mycoplasmas. Veterinary Microbiology, London, v. 126, n. 1-3, p. 132-41, 2008. DOI: 10.1016/j.vetmic.2007.06.018
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