In Brazil, there is a need to balance urbanization with environmental preservation, conserving biomes such as the Atlantic Forest, a hotspot threatened in the Brazil and in the state of Santa Catarina. This study analyzed changes in the Atlantic Forest biome in Joinville, between 2001 and 2022, using data from Collection 8 of MapBiomas and IBGE. Methodologically, the descriptive and exploratory research performed statistical and multivariate analyses, through Principal Component Analysis (PCA), to identify factors with greater correlation. The results show few changes in the areas of Forest Formation (+0.09%), Wooded Restinga (-1.1%) and Water Bodies (-5.8%). However, there was a strong reduction in Mangroves (-14.4%), Non-Forest Natural Formation (-53.8%) and Agriculture (-44.5%). Urban Infrastructure (+24.2%) and Non-Vegeted Areas (+38.5%) grew significantly, indicating the impact of urbanization on the biome. PCA showed a significant correlation in relation to the variables Non-Forest Natural Formation, Agriculture, Deforestation and Water (2001 to 2010), while, from 2017 to 2022, a correlation was evidenced between Gross Domestic Product, Population and Non-Vegeted Area. Forest Formation did not show any correlation with any variable, with 2011 to 2016 being the period of greatest variation.