Sequencial passages of the multiple nucleopolyhedrovirus of Anagrapha falcifera(Kirby) (Lepidoptera: Noctuidae) (AfMNPV) in Spodoptera cosmioides (Walker) (Lepidoptera, Noctuidae)

Maurício Lara Moscardi; Viviane Dutra; Pablo Kruger Fernandes; Samuel Roggia; Sheila Michele Levy; Angela Maria Ferreira Falleiros

doi:10.5433/1679-0367.2021v42n2p221

Autores/as

Maurício Lara Moscardi Universidade Estadual de Londrina - UEL https://orcid.org/0000-0001-5300-699X
Viviane Dutra Universidade Estadual de Londrina - UEL https://orcid.org/0000-0002-3520-5001
Pablo Kruger Fernandes Universidade Estadual de Londrina - UEL https://orcid.org/0000-0001-7614-3487
Samuel Roggia Empresa Brasileira de Pesquisa Agropecuária - Embrapa soja https://orcid.org/0000-0002-9707-1766
Sheila Michele Levy Universidade Estadual de Londrina - UEL https://orcid.org/0000-0002-3043-7655
Angela Maria Ferreira Falleiros Universidade Estadual de Londrina - UEL https://orcid.org/0000-0002-8982-9709

DOI:

https://doi.org/10.5433/1679-0367.2021v42n2p221

Palabras clave:

Morphology, Histopathology, Biological control, Insect viruses

Resumen

The aim of this work was to evaluate the effects of Anagrapha falcifera (AfMNPV) multiple nucleopolyhedrovirus passages in the S. cosmioides caterpillar’s biology at different times of infection and histological changes that the virus could cause in the caterpillar midgut, seeking correlate histopathologic effects to the effectiveness of this virus as a potential biological control of this pest. Larvae were infected with seven days of development, by using three different passages of AfMNPV on S. cosmioides (F1, F4 and F7, which is the first, fourth and seventh passages, respectively) and the control treatment. Compared biology assays with the same treatments for analyzing behavior and mortality of caterpillars were performed concomitantly. The midgut morphology was compared between infected and uninfected larvae. The digestive tubes were collected at 24, 72 and 144 hours of infection (20 tubes/treatment/time of infection). After collection, the digestive tubes were fixed in Karnovsky, processed, stained with Hematoxylin-Eosin, and examined under a light microscope. The biology results of F4 and F7treatments, showed a drastic reduction in locomotion and feeding from the fourth day after infection and higher cumulative mortality rate compared to the control and F1. All treatments caused morphological changes in the midgut of S. cosmioides, in the three times of infection, with the greatest changes occurring at the epithelium. The AfMNPV, in the three passages tested in S. cosmioides, caused behavioral and morphological changes in the midgut, indicating that it can be a promising agent for biological control of this pest.

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Biografía del autor/a

Maurício Lara Moscardi, Universidade Estadual de Londrina - UEL

Doctoral candidate in Agronomy (Phytosanitary/Entomology) by the Graduate Program of the Universidade Estadual de Londrina, Londrina, Paraná, Brazil.

Viviane Dutra, Universidade Estadual de Londrina - UEL

Doctorate in Agronomy (Phytosanitary/Entomology) by the Graduate Program of the Universidade Estadual de Londrina, Londrina, Paraná, Brazil. Technical Manager, Andermatt do Brasil Soluções Biológica, Curitiba, Paraná

Pablo Kruger Fernandes, Universidade Estadual de Londrina - UEL

Biólogo pela Universidade Estadual de Londrina, Londrina, Paraná

Samuel Roggia, Empresa Brasileira de Pesquisa Agropecuária - Embrapa soja

PhD in Entomology from the Luiz de Queiros School of Agriculture, Universidade def São Paulo, Piracicaba. Researcher at Embrapa Soja, Entomology Sector, Londrina, Paraná

Sheila Michele Levy, Universidade Estadual de Londrina - UEL

Doctorate in Biological Sciences (Zoology) from Universidade Estadual Paulista - Júlio de Mesquita Filho, Botucatu. Professor, Department of Histology, Center for Biological Sciences, SUniversidade Estadual de Londrina, Londrina, Paraná

Angela Maria Ferreira Falleiros, Universidade Estadual de Londrina - UEL

Doctorate in Biological Sciences (Human Anatomy) from Universidade Estadual Paulista - Júlio de Mesquita Filho, Botucatu, São Paulo. Professor, Department of Histology, Center for Biological Sciences, Universidade Estadual de Londrina.

Citas

Bavaresco A, Garcia MS, Grutzmacher AD, Ringenberg R, Foresti J. Biologia comparada de Spodoptera cosmioides (Walk.) (Lepidoptera: Noctuidae) em cebola, mamona, soja e feijão. Cienc. Rural. 2003;33(6):993-8.

Bavaresco A, Garcia MS, Grützmacher AD, Ringenberg R, Foresti J. Adequação de uma dieta artificial para a criação de Spodoptera cosmioides (Walk.) (Lepidoptera: Noctuidae) em Laboratório. Neotrop. Entomol. 2004;33(2):155-61.

Billingsley PF, Lehane MJ. Structure and ultrastructure of the insect midgut. In: Lehane MJ, Billingsley PF, editors. Biology of the Insect Midgut. London: Chapman & Hall; 1996. p. 3-30.

Cavalcante VM, Cruz-Landim C. Types of cells present in the midgut of the insects: a review. Naturalia. 1999;24:19-39.

Chapman, R. F. The insects: structure and function. 5a ed. Cambridge: Harvard University Press; 2013.

Correia AA, Wanderley-Teixeira V, Teixeira AAC, Oliveira JV. Morfologia do canal alimentar de lagartas de Spodoptera frugiperda (J E Smith) (Lepidoptera: Noctuidae) alimentadas com folhas tratadas com nim. Neotrop. Entomol. 2001;38(1):83-91.

Cruz NA, Hoffmann-Campo CB, Levy SM, Falleiros AMF. Post-ingestive effects of flavonoids in the midgut epithelium of Anticarsia gemmatalis (Hübner 1818) (Lepidoptera: Erebidae) larvae. Semina: Ciênc. Biol. Saúde. 2017;38(2):185-92.

Eisemann CH, Binnington KC. The peritrophic membrane: Its formation, structure, chemical composition and permeability in relation to vaccination against ectoparasitic arthropods. Int. J. Parasitol. 1994;24(1):15-26.

Engelhard EK, Volkman LE. Developmental resistance in fourth instar Trichoplusi ani orally inoculated with Autographa californica M nuclear polyhedrosisvirus. Virology. 1995;209(2):384-9.

Fialho MCQ, Zanuncio JC, Neves CA, Ramalho FS, Serrão JE. Ultrastructure of the digestive cells in the midgut of the predator Brontocoris tabidus (Heteroptera: Pentatomidae) after different feeding periods on prey and plants. Ann. Entomol. Soc. Am. 2009;102:119-27.

Habib MEM, Paleari LM, Amaral MEC. Effect of three larval diets on the development of the armyworm, Spodoptera latifascia Walker, 1856 (Noctuidae: Lepidoptera). Rev. Bra.de Zool. 1983;1:77-182.

Hakim RS, Baldwin K, Smagghe G. Regulation of midgut growth, development, and metamorphosis. Annu. Rev. Entomol. 2010;55:593-608.

Hakim RS, Baldwin KM, Loeb M. The role of stem cells in midgut growth and regeneration. In vitro cell. Dev. 2001;37(6):338-42.

Hoover K, Washburn JO, Volkman LE. Midgut-based resistance of Heliothis virescens to baculovirus infection mediated by phytochemicals in cotton. J. InsectPhysiol. 2000;46(6):999-1007.

Hostetter DL, Puttler B. A new broad host spectrum nuclear polyhedrosisvirus isolated from a celery looper, Anagrapha falcifera (Kirby), (Lepidoptera: Noctuidae). Environ. Entomol. 1991;20:1480-88.

Keddie BA, Aponte GW, Volkman LE. The pathway of infection of Autographa californica nuclear polyhedrosisvirus in an insect host. Science. 1989;243(4899):1728-30.

Levy SM, Falleiros AMF, Gregório EA, Arrebola NR, Toledo LA. The larval midgut of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae): light and electron microscopy studies of the epithelial cells. J. Biol. 2004;64(3):1-6.

Levy SM, Moscardi F, Falleiros AMF, Silva RJ, Gregório EA. A morphometric study of the midgut in resistant and non-resistant Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) larvae to its nucleopolyhedrovirus (AgMNPV). J. Invertebr. Pathol. 2009;101:17-22.

Loeb MJ, Martin PAW, Narang N, Hakim RS, Goto S, Takeda M. Control of life, death, and differentiation in cultured midgut cells of the lepidopteran, Heliothis virescens. In Vitro Cell. Dev – An. 2001;37(6):348-52.

Morales L, Moscardi F, Gravena S. Potencial do baculovírus de Autographa californica (Speyer) no controle de Chrysodeixis includens (Walker) e Anticarsia gemmatalis Hübner (Lep.:Noctuidae). Pesq. Agropec. Bras. 1993;28(2):237-43.

Mordue (Luntz) AJ, Blackwell A. Azadirachtin: An update. J. Insect Physiol. 1993;39:903-24.

Mordue (Luntz) AJ, Nisbet AJ. Azadirachtin form the neem tree Azadirachta indica: its action against insects. An. Soc. Entomol. Bra. 2000;29:615-32.

Moscardi F, Cunha F, Moscardi ML. Vírus entomopatogênicos como componentes de programas de manejo integrado de pragas. Cienc. Amb. 2011;43.

Moscardi F. Assessment of the applications of baculoviruses for control of Lepidoptera. Annu. Rev. Entomol. 1999;44:257-89.

Moscardi F. Utilização de vírus para controle da lagarta da soja. In: Alves SB, editor. Controle microbiano de insetos. São Paulo: Manole; 1986.p.188-202.

Okuda K, Almeida F, Mortara RA, Krieger H, Marinotti O, Bijovsky AT. Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus. J. Insect Physiol. 2007;53(12):1307-15.

Pinheiro DO, Quagio-Grassiotto I, Gregório EA. Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae. Neotrop. Entomol. 2008;37(4):413-9.

Piovesan M, Specht A, Carneiro E, Paula-Moraes SV, Casagrande MM. Phenological patterns of Spodoptera Guenée, 1852 (Lepidoptera: Noctuidae) is more affected by ENSO than seasonal factors and host plant availability in a Brazilian Savanna. Int. J. of Biometeorol. 2018;62:413-22.

Santos CD, Ribeiro AF, Ferreira C, Terra WR. The larval midgut of cassava hornworm (Erinnyis ello): ultrastructure, fluid flux and the secretory activity in relation to the organization of digestion. Cell Tissue Res. 1984;237:565-74.

Sousa MEC, Santos FAB, Wanderley-Teixeira V, Teixeira AAC, Siqueira HAA, Alves LC, et al. Histopathology and ultrastructure of midgut of Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) fed Bt-cotton. J. Insect Physiol. 2010;56(12);1913-19.

Szewczyk B, Rabalski L, Krol E, Sihler W, Souza ML. Baculovirus biopesticides: a safe alternative to chemical protection of plants. J. Biopest. 2009;2:209-16.

Tellam RL. The peritrophic matrix. In: Lehane MJ, Billingsley PF (Eds.) Biology of the Insect Midgut. London: Chapman & Hall;1996. p. 86-114.

Terra W. The origin and functions of the insect peritrophic membrane and peritrophic gel. Arch. Insect Biochem. Physiol. 2001;47: 47-61.

Wang P, Granados RR. Molecular structure of the peritrophic membrane (PM): Identification of potential PM target sites for insect control. Arch. Insect Biochem. Physiol. 2001;47(2):110-8.

Washburn JO, Wong JF, Volkman LE. Comparative pathogenesis of Helicoverpa zea nucleopolyhedrovirus in noctuid larvae. J. Gen. Virol. 2001;82(7):1777-84.