Tolerance of eukaryotic microorganisms to glyphosate herbicide
DOI:
https://doi.org/10.5433/1679-0367.2021v42n1p103Keywords:
Filamentous fungi, Yeast. Bacteria, Soil, BioremediationAbstract
Glyphosate is one of the most widely used herbicides. The World Health Organization (WHO) has reclassified glyphosate as "probably carcinogenic to humans". Glyphosate removal from the environment can be performed by microbial enzymatic action. The present work focused on the isolation of soil microorganisms that can tolerate glyphosate as the sole carbon source. Cells were isolated in minimal culture medium supplemented with glyphosate. Microbial biomass production was verified in the presence and absence of glyphosate. Seventeen, fourteen and one bacteria, fungi and yeast were isolated, respectively. One fungus (F3) and one yeast (L1), were selected after glyphosate tolerance test in liquid medium. Eukaryotic microorganisms tolerate glyphosate, however metabolism was affected by herbicide compared to control without glyphosate. Statistically growth time showed significant differences. Eukaryotic microorganisms isolated from soil with glyphosate are tolerant to the compound and may be useful as bioremediators of environments affected by this herbicide.Downloads
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References
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Ramos GFC, Ramos PL, Passarini MRZ, Silveira MAV, Okamoto DN, Oliveira LCG et al. Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus. AMB Expr. 2016; 6(1):17. doi: 10.1186/s13568-016-0188-x.
Ratcliff A, Busse M, Shestak, C. Changes in microbial community structure following herbicide (glyphosate) additions to forest soils. Appl Soil Ecol. 2006;34(2-3):114-24. doi: https://doi.org/10.1016/j.apsoil.2006.03.002.
Andrighetti MS, Nachtigall GR, Queiroz SCN, Ferracini VL, Ayub MAZ. Biodegradação de glifosato pela microbiota de solos cultivados com macieira. R. Bras. Ci. Solo. 2014;38:1643-53.
Balbuena MS, Tison L, Hahn ML, Greggers U, Menzel R, Farina WM. Effects of sublethal doses of glyphosate on honeybee navigation. J Exp Biol. 2015;218(17):2799-805. doi: 10.1242/jeb.117291.
Batista E, Ottoni JR, Sartoratto A, Oliveira VM, Passarini MRZ. Hidrocarbonetos produzidos por bactérias da Antártica. Rev Biotecnol Ciênc. 2018;7(2):38-46.
Conrad A, Schröter-Kermani C, Hoppe HW, Rüther M, Pieper S, Kolossa-Gehring M. Glyphosate in German adults – Time trend (2001 to 2015) of human exposure to a widely used herbicide. Int J Hyg Envir Heal. 2017;220(1):8-16. Doi: 10.1016/j.ijheh.2016.09.016.
Eman A, Abdel-Megeed A, Suliman AMR, Sadik MW, Sholkamy EN. Biodegradation of Glyphosate by fungal strains isolated from herbicides polluted-soils in Riyadh area. Int J Curr Microbiol App Sci. 2013;2(8):359-81.
European Food Safety Authority (EFSA). Conclusion on the peer review of the pesticide risk assessment of the active substance glyphosate. EFSA J. 2015;13(11):4302.
Fernandes MCS, Costa LS, Grazziotti PH, Grazziotti DCFS, Santos JB, Rossi MJ. Pisolithus sp. Tolerance to glyphosate and isoxaflutole In Vitro. Rev Árvore. 2014;38(3):461-8.
Food and Agriculture Oorganization of the United Nations (FAO). World agriculture: towards 2015-2030 an FAO Perspective. London: Earthscan Publications; 2003.
Greim H, Saltmiras D, Mostert V, Strupp C. Evaluation of carcinogenic potential of the herbicide glyphosate, drawing on tumor incidence data from fourteen chronic/carcinogenicity rodent studies. Crit Rev Toxicol. 2015;45(3):185-208. doi: 10.3109/10408444.2014.1003423.
Guyton KZ, Loomis D, Grosse Y, El Ghissassi F, Benbrahim-Tallaa L, Guha N et al. Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate. Lancet Oncol. 2015;16(5):490-4. doi: 10.1016/S1470-2045(15)70134-8
Jurado AS, Fernandes MAS, Videira RA, Peixoto FP, Vicente JAF. Herbicides: the Face and the Reverse of the Coin. An in vitro Approach to the Toxicity of Herbicides in Non-Target Organisms. In: Kortekamp A, editor. Herbicides and Environment. London: InTech; 2011. p. 1-44.
Laborde A, Tomasina F, Bianchi F, Bruné MN, Buka I, Comba P et al. Children’s Health in Latin America: The Influence of Environmental Exposures. Environ Health Perspec. 2015;123(3):201-9. doi: 10.1289/ehp.1408292.
Lane M, Lorenz N, Saxena J, Ramsier C, Dick RP. The effect of glyphosate on soil microbial activity, microbial community structure, and soil potassium. Pedobiologia. 2012;55(6): 335-42.
Low FL, Shaw IC, Gerrard JA. The effect of Saccharomyces cerevisiae on the stability of the herbicide glyphosate during bread leavening. Lett Appl Microbiol. 2005;40(2): 133-7. doi: 10.1111/j.1472-765X.2004.01633.x.
Manogaran M, Shukor MY, Yasid, NA, Johari WLW, Ahmad SA. Isolation and characterisation of glyphosate-degrading bacteria isolated from local soils in Malaysia. Rend Fis Acc Lincei. 2017;28(3):471-9. doi 10.1007/s12210-017-0620-4.
Monteiro P, Beneduzi A, Sampaio J, Vargas LK, Lisboa B. Isolamento de linhagens bacterianas do solo capazes de crescer em altas concentrações de glifosato. Tecnol. & Ciên. Agropec. 2017;11(3):37-42.
Moraes PVD, Rossi P. Comportamento ambiental do glifosato. Sci Agrar Parana. 2010; 9(3): 22-35.
Motta EVS, Raymann K, Moran NA. Glyphosate perturbs the gut microbiota of honey bees. PNAS. 2018;115(41):10305-10. doi: Org/10.1073/Pnas.1803880115
Ottoni JR, Silva TR, Oliveira VM, Passarini MRZ. Characterization of amylase produced by cold-adapted bacteria from Antarctic samples. Biocatal Agric Biotechnol. 2020;23:101452. doi: https://doi.org/10.1016/j.bcab.2019.101452.
Paganelli A, Gnazzo V, Acosta H, López SL, Carrasco AE. Glyphosate-Based Herbicides Produce Teratogenic Effects on Vertebrates by Impairing Retinoic Acid Signaling. Chem Res Toxicol. 2010;23(10):1586-95. doi: 10.1021/tx1001749.
Pedotti M, Rosini E, Molla G, Moschetti T, Savino C, Vallone B et al. Glyphosate Resistance by Engineering the Flavoenzyme Glycine Oxidase. J Biol Chem. 2009;284(52):36415-23. doi: 10.1074/jbc.M109.051631.
Pollegioni L, Schonbrunn E, Siehl D. Molecular basis of glyphosate resistance-different approaches through protein engineering. FEBS J. 2011;278(16):2753-66. doi: 10.1111/j.1742-4658.2011.08214.x.
Qasem J. Herbicides Applications: Problems and Considerations. In: Kortekamp A, editor. Herbicides and Environment. London: InTech; 2011. p. 643-4. doi: 10.5772/550
Ramos GFC, Ramos PL, Passarini MRZ, Silveira MAV, Okamoto DN, Oliveira LCG et al. Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus. AMB Expr. 2016; 6(1):17. doi: 10.1186/s13568-016-0188-x.
Ratcliff A, Busse M, Shestak, C. Changes in microbial community structure following herbicide (glyphosate) additions to forest soils. Appl Soil Ecol. 2006;34(2-3):114-24. doi: https://doi.org/10.1016/j.apsoil.2006.03.002.
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Published
2021-02-02
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Nogueira OMN, Salgueiro JLG, Francisco EA, Ottoni JR, Passarini MRZ. Tolerance of eukaryotic microorganisms to glyphosate herbicide. Semin. Cienc. Biol. Saude [Internet]. 2021 Feb. 2 [cited 2024 Nov. 21];42(1):103-12. Available from: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/41979
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