Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses
DOI:
https://doi.org/10.5433/1679-0359.2022v43n2p713Palabras clave:
Complex Mycobacterium tuberculosis, Nested q-PCR, Post mortem inspection, Beef.Resumen
Mycobacterium bovis is a bacterium belonging to the Mycobacterium tuberculosis complex that causes tuberculosis in cattle and in other domestic and wild animals, as well as in humans. Disease control measures are carried out by slaughtering animals tested positive in the intradermal tuberculinization test and sanitation of their original living spaces, in addition to epidemiological surveillance carried out through the sanitary inspection of bovine carcasses in slaughterhouses. In the latter, official inspection services collect samples from macroscopic lesions suspected of bovine tuberculosis, which are then sent for laboratory analysis. Knowledge concerning the variables associated with the occurrence of M. bovis can aid in decision-making regarding control and disease eradication efforts. In this context, the aim of this study was to identify the risk factors for a positive M. bovis diagnosis in suspected bovine tuberculosis lesions obtained during epidemiological surveillance activities in the state of Mato Grosso, Brazil. A total of 105 suspicious lesions were analyzed using the Nested Polymerase Chain Reaction (nested q-PCR) method, of which 14 (13.33%) tested positive for M. bovis. Univariate and bivariate statistical analyses indicated that the variable “animal slaughter” was the only risk factor presenting statistical significance associated with the diagnosis of M. bovis (p < 0.05), demonstrating that macroscopic lesions suspected as being caused by bovine tuberculosis from animals with an in vivo diagnosis were 2.82 - fold more likely to result in a positive M. bovis diagnosis by molecular tests.Citas
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Araújo, C. P., Osorio, A. L. A., Jorge, K. S., Ramos, C. A. N., Francisco, S. A. Fo., Vidal, C. E. S.,... Araújo, F. R. (2014a). Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. PLoS One, 9(3). doi: 10.1371/journal.pone.0091023
Araújo, C. P., Osório, A. L. A., Jorge, K. S., Ramos, C. A., Souza, A. F., Fº., Vidal, C. E.,... Araújo, F. R. (2014b). Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology, 45(2), 633-640. doi: 10.1590/S1517-83822014000200 035
Azami, H. Y., & Zinsstag, J. (2018). Economics of bovine tuberculosis: a one-healt issue. In M. Chambers, S. Gordon, F. Olea-Popelka, & P. Barrow (Orgs.), Bovine tuberculosis (cap. 3, p. 31-42). London, UK: British Library.
Biffa, D., Inangolet, F., Bogale, A., Oloya, J., Djønne, B., & Skjerve, E. (2011). Risk factors associated with prevalence of tuberculosis-like lesions and associated mycobacteria in cattle slaughtered at public and export abattoirs in Ethiopia. Tropical Animal Health and Production, 43(2), 529-538. doi: 10.1007/s11 250-010-9729-5
Biffa, D., Bogale, A., Godfroid, J., & Skjerve, E. (2012). Factors associated with severity of bovine tuberculosis in Ethiopian cattle. Tropical Animal Health and Production, 44(5), 991-998. doi: 10.1007/s 11250-011-0031-y
Boukary, A. R., Thys, E., Rigouts, L., Matthys, F., Berkvens, D., Mahamadou, I.,… Saegerman, C. (2012). Risk factors associated with bovine tuberculosis and molecular characterization of Mycobacterium bovis strains in urban settings in Niger. Transboundary and Emerging Diseases, 59(6), 490-502. doi: 10.1111/ j.1865-1682.2011.01302.x
RIISPOA (2017). Regulamento da Inspeção Industrial e Sanitária de Produtos de Origem Animal (RIISPOA). Ministério da Agricultura, Pecuária e Abastecimento. Recuperado de https://www.gov.br/ agricultura/pt-br/assuntos/inspecao/produtos-animal/arquivos/decreto-n-9013-2017_lt-decreto-9069-20 17_pt.pdf/view
Carvalho, R. C. T., Furlanetto, L. V., Maruyama, F. H., Araújo, C. P. de, Barros, S. L. B., Nascimento Ramos, C. A. do,... Figueiredo, E. E. S. (2015). Evaluation of the efficiency of nested q-PCR in the detection of Mycobacterium tuberculosis complex directly from tuberculosis-suspected lesions in post-mortem macroscopic inspections of bovine carcasses slaughtered in the state of Mato Grosso, Brazil. Meat Science, 106, 11-15. doi: 10.1016/j.meatsci.2015.03.017
Carvalho, R. C. T., Vasconcellos, S. E. G., Azevedo, I. S. S. A. de M., Soares, P. M., Fº., Mota, P. M. P. C., Araújo, F. R. de,... Paschoalin, V. M. F. (2016). Molecular typing of Mycobacterium bovis from cattle reared in Midwest Brazil. PloS One, 11(9), 1-16. doi: 10.1371/journal.pone.0162459
Cazola, D. D. O., Jorge, K. D. S., Zumárraga, M. J., Souza, A. F., Fº., Araújo, F. R., & Osório, A. L. A. (2015). Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul. Pesquisa Veterinária Brasileira, 35(2), 141-147. doi: 10.1590/S0 100-736X2015000200008
Clegg, T. A., Good, M., & More, S. J. (2016). Risk factors for cattle presenting with a confirmed bTB lesion at slaughter, from herds with no evidence of within-herd transmission. Preventive Veterinary Medicine, 126(1), 111-120. doi: 10.1016/j.prevetmed.2016.02.003
Figueiredo, E. E. de S., Silvestre, F. G., Campos, W. N., Furlanetto, L. V., Medeiros, L., Lilenbaum, W.,… Paschoalin, V. M. F. (2009). Identification of Mycobacterium bovis isolates by a multiplex PCR. Brazilian Journal of Microbiology, 40(2), 231-233. doi: 10.1590/S1517-83822009000200004
Figueiredo, E. E. de S., Conte-Junior, C. A., Furlanetto, L. V., Silva, F. G. S., Duarte, R. S., Silva, J. T.,... Paschoalin, V. M. F. (2012). Molecular techniques for identification of species of the Mycobacterium tuberculosis complex: the use of multiplex PCR and an adapted HPLC method for identification of Mycobacterium bovis and diagnosis of bovine tuberculosis. In P.-J. Cardona (Ed.), Understanding tuberculosis: global experiences and innovative approaches to the diagnosis (pp. 411-432). InTech Open.
Fráguas, A. S., Cunha-Abreu, M. S., Reis Ferreira, A. M. dos, Marassi, C. D., Oelemann, W., Souza Fonseca, L. de,... Lilenbaum, W. (2008). Estudo comparativo de métodos complementares para o diagnóstico da tuberculose bovina em animais reagentes à tuberculinização. Revista Brasileira de Ciência Veterinária, 15(3), 117-121. doi: 10.4322/rbcv.2014.211 Recuperado de https://periodicos.uff. br/rbcv/article/view/ 7073
Portaria Conjunta SEDRAF-INDEA/MT n. 009 (2014). Portaria Conjunta SEDRAF-INDEA/MT n. 009, 14 de novembro de 2014. Institui o regulamento técnico do plano de vigilância para a Erradicação da Tuberculose bovina no Estado de Mato Grosso e cria o Programa Estadual de Submissão de Granuloma do Estado de Mato Grosso. Diário Oficial do Estado de Mato Grosso. Cuiabá, MT, 13 nov. 2014. Recuperado de https://www.legisweb.com.br/ legislacao/? id =276860
Medeiros, L. D. S., Marassi, C. D., Figueiredo, E. E. S., & Lilenbaum, W. (2010). Potential application of new diagnostic methods for controlling bovine tuberculosis in Brazil. Brazilian Journal of Microbiology, 41(3), 531-541. doi: 10.1590/S1517-83822010005000002
Negreiros, R. L., Dias, R. A., Ferreira, F., Ferreira, J. S., Neto, Gonçalves, V. S. P., Silva, M. D. C. P.,... Amaku, M. (2009). Situação epidemiológica da brucelose bovina no Estado de Mato Grosso. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61(1), 56-65. doi: 10.1590/S0102-09352009000700007
Néspoli, J. M. B., Negreiros, R. L., Amaku, M., Dias, R. A., Ferreira, F., Telles, E. O., Ferreira, J. S. Neto. (2016). Epidemiological situation of bovine tuberculosis in the state of Mato Grosso, Brazil. Semina: Ciências Agrárias, 37(5), 3589-3599. doi: 10.5433/1679-0359.2016v37n5Supl2p3589
Okeke, L. A., Cadmus, S., Okeke, I. O., Muhammad, M., Awoloh, O., Dairo, D.,… Fawole, O. (2014). Prevalence and risk factors of Mycobacterium tuberculosis complex infection in slaughtered cattle at Jos South Abattoir, Plateau State, Nigeria. The Pan African Medical Journal, 18(Suppl. 1), 1-5. doi: 10.116 94/pamj.supp.2014.18.1.3841
Pascual-Linaza, A. V., Gordon, A. W., Stringer, L. A., & Menzies, F. D. (2017). Efficiency of slaughterhouse surveillance for the detection of bovine tuberculosis in cattle in Northern Ireland. Epidemiology & Infection, 145(5), 995-1005. doi: 10.1017/S0950268816003095
Shittu, A., Clifton-Hadley, R. S., Ely, E. R., Upton, P. U., & Downs, S. H. (2013). Factors associated with bovine tuberculosis confirmation rates in suspect lesions found in cattle at routine slaughter in Great Britain, 2003-2008. Preventive Veterinary Medicine, 110(3-4), 395-404. doi: 10.1016/j.prevetmed.2013. 03.001
Zarden, C. F. O., Marassi, C. D., Carvalho, A. C., Figueiredo, E. E. S., & Lilenbaum, W. (2013). Bacteriological and molecular detection of Mycobacterium bovis in cattle with inconclusive results to intradermal tuberculin tests. Epidemiology & Infection, 141(7), 1390-1393. doi: 10.1017/S0950268812 002105
Araújo, C. P., Osorio, A. L. A., Jorge, K. S., Ramos, C. A. N., Francisco, S. A. Fo., Vidal, C. E. S.,... Araújo, F. R. (2014a). Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. PLoS One, 9(3). doi: 10.1371/journal.pone.0091023
Araújo, C. P., Osório, A. L. A., Jorge, K. S., Ramos, C. A., Souza, A. F., Fº., Vidal, C. E.,... Araújo, F. R. (2014b). Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology, 45(2), 633-640. doi: 10.1590/S1517-83822014000200 035
Azami, H. Y., & Zinsstag, J. (2018). Economics of bovine tuberculosis: a one-healt issue. In M. Chambers, S. Gordon, F. Olea-Popelka, & P. Barrow (Orgs.), Bovine tuberculosis (cap. 3, p. 31-42). London, UK: British Library.
Biffa, D., Inangolet, F., Bogale, A., Oloya, J., Djønne, B., & Skjerve, E. (2011). Risk factors associated with prevalence of tuberculosis-like lesions and associated mycobacteria in cattle slaughtered at public and export abattoirs in Ethiopia. Tropical Animal Health and Production, 43(2), 529-538. doi: 10.1007/s11 250-010-9729-5
Biffa, D., Bogale, A., Godfroid, J., & Skjerve, E. (2012). Factors associated with severity of bovine tuberculosis in Ethiopian cattle. Tropical Animal Health and Production, 44(5), 991-998. doi: 10.1007/s 11250-011-0031-y
Boukary, A. R., Thys, E., Rigouts, L., Matthys, F., Berkvens, D., Mahamadou, I.,… Saegerman, C. (2012). Risk factors associated with bovine tuberculosis and molecular characterization of Mycobacterium bovis strains in urban settings in Niger. Transboundary and Emerging Diseases, 59(6), 490-502. doi: 10.1111/ j.1865-1682.2011.01302.x
RIISPOA (2017). Regulamento da Inspeção Industrial e Sanitária de Produtos de Origem Animal (RIISPOA). Ministério da Agricultura, Pecuária e Abastecimento. Recuperado de https://www.gov.br/ agricultura/pt-br/assuntos/inspecao/produtos-animal/arquivos/decreto-n-9013-2017_lt-decreto-9069-20 17_pt.pdf/view
Carvalho, R. C. T., Furlanetto, L. V., Maruyama, F. H., Araújo, C. P. de, Barros, S. L. B., Nascimento Ramos, C. A. do,... Figueiredo, E. E. S. (2015). Evaluation of the efficiency of nested q-PCR in the detection of Mycobacterium tuberculosis complex directly from tuberculosis-suspected lesions in post-mortem macroscopic inspections of bovine carcasses slaughtered in the state of Mato Grosso, Brazil. Meat Science, 106, 11-15. doi: 10.1016/j.meatsci.2015.03.017
Carvalho, R. C. T., Vasconcellos, S. E. G., Azevedo, I. S. S. A. de M., Soares, P. M., Fº., Mota, P. M. P. C., Araújo, F. R. de,... Paschoalin, V. M. F. (2016). Molecular typing of Mycobacterium bovis from cattle reared in Midwest Brazil. PloS One, 11(9), 1-16. doi: 10.1371/journal.pone.0162459
Cazola, D. D. O., Jorge, K. D. S., Zumárraga, M. J., Souza, A. F., Fº., Araújo, F. R., & Osório, A. L. A. (2015). Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul. Pesquisa Veterinária Brasileira, 35(2), 141-147. doi: 10.1590/S0 100-736X2015000200008
Clegg, T. A., Good, M., & More, S. J. (2016). Risk factors for cattle presenting with a confirmed bTB lesion at slaughter, from herds with no evidence of within-herd transmission. Preventive Veterinary Medicine, 126(1), 111-120. doi: 10.1016/j.prevetmed.2016.02.003
Figueiredo, E. E. de S., Silvestre, F. G., Campos, W. N., Furlanetto, L. V., Medeiros, L., Lilenbaum, W.,… Paschoalin, V. M. F. (2009). Identification of Mycobacterium bovis isolates by a multiplex PCR. Brazilian Journal of Microbiology, 40(2), 231-233. doi: 10.1590/S1517-83822009000200004
Figueiredo, E. E. de S., Conte-Junior, C. A., Furlanetto, L. V., Silva, F. G. S., Duarte, R. S., Silva, J. T.,... Paschoalin, V. M. F. (2012). Molecular techniques for identification of species of the Mycobacterium tuberculosis complex: the use of multiplex PCR and an adapted HPLC method for identification of Mycobacterium bovis and diagnosis of bovine tuberculosis. In P.-J. Cardona (Ed.), Understanding tuberculosis: global experiences and innovative approaches to the diagnosis (pp. 411-432). InTech Open.
Fráguas, A. S., Cunha-Abreu, M. S., Reis Ferreira, A. M. dos, Marassi, C. D., Oelemann, W., Souza Fonseca, L. de,... Lilenbaum, W. (2008). Estudo comparativo de métodos complementares para o diagnóstico da tuberculose bovina em animais reagentes à tuberculinização. Revista Brasileira de Ciência Veterinária, 15(3), 117-121. doi: 10.4322/rbcv.2014.211 Recuperado de https://periodicos.uff. br/rbcv/article/view/ 7073
Portaria Conjunta SEDRAF-INDEA/MT n. 009 (2014). Portaria Conjunta SEDRAF-INDEA/MT n. 009, 14 de novembro de 2014. Institui o regulamento técnico do plano de vigilância para a Erradicação da Tuberculose bovina no Estado de Mato Grosso e cria o Programa Estadual de Submissão de Granuloma do Estado de Mato Grosso. Diário Oficial do Estado de Mato Grosso. Cuiabá, MT, 13 nov. 2014. Recuperado de https://www.legisweb.com.br/ legislacao/? id =276860
Medeiros, L. D. S., Marassi, C. D., Figueiredo, E. E. S., & Lilenbaum, W. (2010). Potential application of new diagnostic methods for controlling bovine tuberculosis in Brazil. Brazilian Journal of Microbiology, 41(3), 531-541. doi: 10.1590/S1517-83822010005000002
Negreiros, R. L., Dias, R. A., Ferreira, F., Ferreira, J. S., Neto, Gonçalves, V. S. P., Silva, M. D. C. P.,... Amaku, M. (2009). Situação epidemiológica da brucelose bovina no Estado de Mato Grosso. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61(1), 56-65. doi: 10.1590/S0102-09352009000700007
Néspoli, J. M. B., Negreiros, R. L., Amaku, M., Dias, R. A., Ferreira, F., Telles, E. O., Ferreira, J. S. Neto. (2016). Epidemiological situation of bovine tuberculosis in the state of Mato Grosso, Brazil. Semina: Ciências Agrárias, 37(5), 3589-3599. doi: 10.5433/1679-0359.2016v37n5Supl2p3589
Okeke, L. A., Cadmus, S., Okeke, I. O., Muhammad, M., Awoloh, O., Dairo, D.,… Fawole, O. (2014). Prevalence and risk factors of Mycobacterium tuberculosis complex infection in slaughtered cattle at Jos South Abattoir, Plateau State, Nigeria. The Pan African Medical Journal, 18(Suppl. 1), 1-5. doi: 10.116 94/pamj.supp.2014.18.1.3841
Pascual-Linaza, A. V., Gordon, A. W., Stringer, L. A., & Menzies, F. D. (2017). Efficiency of slaughterhouse surveillance for the detection of bovine tuberculosis in cattle in Northern Ireland. Epidemiology & Infection, 145(5), 995-1005. doi: 10.1017/S0950268816003095
Shittu, A., Clifton-Hadley, R. S., Ely, E. R., Upton, P. U., & Downs, S. H. (2013). Factors associated with bovine tuberculosis confirmation rates in suspect lesions found in cattle at routine slaughter in Great Britain, 2003-2008. Preventive Veterinary Medicine, 110(3-4), 395-404. doi: 10.1016/j.prevetmed.2013. 03.001
Zarden, C. F. O., Marassi, C. D., Carvalho, A. C., Figueiredo, E. E. S., & Lilenbaum, W. (2013). Bacteriological and molecular detection of Mycobacterium bovis in cattle with inconclusive results to intradermal tuberculin tests. Epidemiology & Infection, 141(7), 1390-1393. doi: 10.1017/S0950268812 002105
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2022-02-25
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Barcelos, F. G., Anjos, T. R. dos, Alencar, L. A. de A., Castro, V. S., Cunha-Neto, A., Carvalho, F. T., … Figueiredo, E. E. de S. (2022). Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses. Semina: Ciências Agrárias, 43(2), 713–726. https://doi.org/10.5433/1679-0359.2022v43n2p713
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