Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses

Authors

  • Lídia Carolina Meira Arneiro Universidade Estadual Paulista
  • Rogério Adallah Curi Universidade Estadual Paulista
  • Josineudson Augusto II Vasconcelos Silva Universidade Estadual Paulista
  • Marcílio Dias Silveira da Mota Universidade Estadual Paulista

DOI:

https://doi.org/10.5433/1679-0359.2012v33n5p2001

Keywords:

CRISP1, Horses, HTR1A, Polymorphisms, SLC6A4.

Abstract

The aims of the present study were to propose a PCR-RFLP genotyping method for the AJ_315378:c.110A>G and AB_264325:c.771G>C SNPs in the equine CRISP1 and HTR1A genes, respectively, as well as to characterize these and another polymorphism, AB_098561:c.1470G>A of the SLC6A4 gene, in order to provide a basis for future studies investigating the association between DNA markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. PCR-RFLP was found to be adequate for the genotyping of the SNPs AJ_315378:c.110A>G of the CRISP1 and AB_264325:c.771G>C of the HTR1A. However, the polymorphism of the CRISP1 probably does not occur in Mangalarga horses, a fact impairing association studies of this marker with traits related to male fertility. The estimative of the population genetic parameters obtained for the polymorphisms AB_264325:c.771G>C of the HTR1A and AB_098561:c.1470G>A of the SLC6A4 in the studied sample discourage the conduct of research addressed the association between markers and traits related to temperament.

Author Biographies

Lídia Carolina Meira Arneiro, Universidade Estadual Paulista

Discente do Deptº de Genética e Melhoramento Animal, Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, UNESP, Jaboticabal, SP.

Rogério Adallah Curi, Universidade Estadual Paulista

Prof. do Deptº de Melhoramento e Nutrição Animal, Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu, SP.

Josineudson Augusto II Vasconcelos Silva, Universidade Estadual Paulista

Prof. do Deptº de Melhoramento e Nutrição Animal, Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu, SP.

Marcílio Dias Silveira da Mota, Universidade Estadual Paulista

Prof. do Deptº de Melhoramento e Nutrição Animal, Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu, SP.

Published

2012-10-30

How to Cite

Arneiro, L. C. M., Curi, R. A., Silva, J. A. I. V., & Mota, M. D. S. da. (2012). Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses. Semina: Ciências Agrárias, 33(5), 2001–2010. https://doi.org/10.5433/1679-0359.2012v33n5p2001

Issue

Section

Articles