Strongyloides venezuelensis DNA is detectable through PCR from feces of experimentally infected rats

Authors

  • Priscilla Duarte Marques Fonseca Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo
  • Natália Albuquerque Soares Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo
  • Gabriela Lima Alvarenga Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo
  • Marcelo Andreetta Corral Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo
  • Larissa Rodrigues Bosqui Universidade Estadual de Londrina
  • Gessica Baptista Melo Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo
  • Susana Angelica Zevallos Lescano Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo
  • Ronaldo César Borges Gryschek Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo
  • Fernanda Mello Malta Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo
  • Larissa Juliani Sanches Universidade Estadual de Londrina
  • Glauco Akelinghton Freire Vitiello Universidade Estadual de Londrina
  • Idessania Nazareth Costa Universidade Estadual de Londrina
  • Fabiana Martins de Paula Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo

Keywords:

molecular diagnosis, Strongyloides, Polymerase chain reaction, helminths

Abstract

Experimental infections with Strongyloides venezuelensis are used as a model for studying human strongyloidiasis, mainly for testing new diagnostic tools. We evaluated the effectiveness of PCR for the detection of S. venezuelensis DNA in samples of blood, serum and feces from experimentally infected rats. S. venezuelensis DNA was amplified in fecal samples. There was no amplification observed in blood or serum samples. It is concluded that PCR can be used in fecal samples for the detection of Strongyloides infections, and can be applicable as a diagnostic test in strongyloidiasis.

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Published

2020-10-28

How to Cite

Fonseca, P. D. M., Soares, N. A., Alvarenga, G. L., Corral, M. A., Bosqui, L. R., Melo, G. B., … de Paula, F. M. (2020). Strongyloides venezuelensis DNA is detectable through PCR from feces of experimentally infected rats. Biosaúde, 22(1), 27–33. Retrieved from https://ojs.uel.br/revistas/uel/index.php/biosaude/article/view/39463

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Artigos