Strongyloides venezuelensis DNA is detectable through PCR from feces of experimentally infected rats
Keywords:
molecular diagnosis, Strongyloides, Polymerase chain reaction, helminthsAbstract
Experimental infections with Strongyloides venezuelensis are used as a model for studying human strongyloidiasis, mainly for testing new diagnostic tools. We evaluated the effectiveness of PCR for the detection of S. venezuelensis DNA in samples of blood, serum and feces from experimentally infected rats. S. venezuelensis DNA was amplified in fecal samples. There was no amplification observed in blood or serum samples. It is concluded that PCR can be used in fecal samples for the detection of Strongyloides infections, and can be applicable as a diagnostic test in strongyloidiasis.
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