Immobilization of lipase in pectin extracted from lobeira fruit (Solanum lycocarpum St. Hil.)
DOI:
https://doi.org/10.5433/2316-5200.2012v1n1p9Keywords:
Pectin, immobilization, Solanum lycocarpum, lipaseAbstract
In this study, pectin extracted from lobeira fruit (Solanum lycocarpum St. Hil) was used as support for lipase immobilization. For the extraction process was utilized a 23 factorial design, with temperature, pH and extraction time as independent variables. Immobilization was performed by adsorption and covalent bonding using sodium periodate (PEC - P) or glutaraldehyde (PEC - G) as activators of support. The results indicated that covalent bonding PEC - P was efficient for the immobilization of the enzyme. The immobilization by adsorption and covalent bonding PEC - G did not provide storage stability. The results of stability tests for the storage system lipase – PEC - P demonstrated that this system maintained 85.4% of enzyme activity after eleven weeks of storage. These results enable the use of pectin extracted from Solanum lycocarpum (St. Hil.) fruit as a promising support for the immobilization of enzymes.