Avaliação de métodos de esterilização, concentração de ágar e composição de meio de cultura para propagação in vitro de Pimpinella anisum (Linn.) – Apiaceae

Gabriela Tambosi; Gladys Daniela Rogge-Renner

doi:10.5433/1679-0367.2010v31n2p189

Evaluation of asepsis, agar concentration and culture medium composition methods for in vitro propagation of Pimpinella anisum (Linn.) – Apiaceae

Authors

Gabriela Tambosi Unidade São Francisco do Sul -UNIVILLE
Gladys Daniela Rogge-Renner Unidade São Francisco do Sul -UNIVILLE

DOI:

https://doi.org/10.5433/1679-0367.2010v31n2p189

Keywords:

Anise, Micro-propagation, Asepsis, Nutrition.

Abstract

Pimpinella anisum, is a plant species popularly used as treatment for different diseases, although no studies exist which could prove its pharmacological activity. This species has no known methods of asepsis and adequate conditions to conduct in vitro propagation trials. To assess the most efficient method of seed sterilization and the most favorable concentration of agar and MS salts, five tests of asepsis and tests to evaluate the dry mass increase of germinated seed in 3g/L and 6g/L of agar and MS in concentrations of 0%, 25%, 50%, 75% and 100%, were carried out. The samples that were immersed in a concentration of 2% sodium hypochlorite for 20 minutes and then washed in distilled water produced 100% of sterile plantlets. The greatest increase in dry mass was obtained with 75% and 100% of MS salts. Furthermore, the different concentrations of agar did not influence the percentage of the plantlets dry mass.

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Author Biographies

Gabriela Tambosi, Unidade São Francisco do Sul -UNIVILLE

Acadêmica do curso de Farmácia, bolsista de iniciação científica da UNIVILLE.

Gladys Daniela Rogge-Renner, Unidade São Francisco do Sul -UNIVILLE

Professora do Departamento de Farmácia e Ciências Biológicas da UNIVILLE.

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Published

2010-12-15

How to Cite

Tambosi G, Rogge-Renner GD. Evaluation of asepsis, agar concentration and culture medium composition methods for in vitro propagation of Pimpinella anisum (Linn.) – Apiaceae. Semin. Cienc. Biol. Saude [Internet]. 2010 Dec. 15 [cited 2024 Nov. 21];31(2):189-94. Available from: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/8798

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Issue

Vol. 31 No. 2 (2010)

Section

Artigos

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