Observação In vitro de Eurytrema coelomaticum em meios de cultivos

Milton Hissashi Yamamura; Aline Artioli Machado Ymamaura; Michael Robin Honer

doi:10.5433/1679-0367.1992v13n2p58

Cultivation in vitro of Eurytrema coelomaticum

Authors

Milton Hissashi Yamamura Universidade Estadual de Londrina
Aline Artioli Machado Ymamaura Universidade Estadual de Londrina
Michael Robin Honer Empresa Brasileira de Pesquisa Agropecuária

DOI:

https://doi.org/10.5433/1679-0367.1992v13n2p58

Keywords:

Euryirema coelomaticum, "In vitro" cultivation, "In vitro" egg laying

Abstract

Culture media Gay ABC, 109 and animate serum have been used in "in vitro" cultivation of Eurytrema coelomaticum. The criterion for recording the survival was through the motility and the observation by means of a stereoscopic microscope. The bust result was obtained by the use of sheep's serum, when it was observed that this trematode con be maintained alive for more than 1200 hours and one of the reasons for this was that ail experiments were conducted under aseptic conditions. Oats on the rale of egg laying by E. coelomaticum were observed. The overage doily rate per worm was 641 eggs. In the first 24 hours, the average was 473 eggs and it increased in 48 hours to 673 eggs per day. The average daily rate of E. coelomaticum was considered low and extremely irregular.

Author Biographies

Milton Hissashi Yamamura, Universidade Estadual de Londrina

Departamento de Medicina Veterinária Preventiva – CCA – Universidade Estadual de Londrina.

Aline Artioli Machado Ymamaura, Universidade Estadual de Londrina

Departamento de Medicina Veterinária Preventiva – CCA – Universidade Estadual de Londrina.

Michael Robin Honer, Empresa Brasileira de Pesquisa Agropecuária

Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA – Campo Grande – MS

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Published

2004-12-15

How to Cite

Yamamura MH, Ymamaura AAM, Honer MR. Cultivation in vitro of Eurytrema coelomaticum. Semin. Cienc. Biol. Saude [Internet]. 2004 Dec. 15 [cited 2024 Aug. 24];13(2):58-60. Available from: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/6969

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Issue

Vol. 13 No. 2 (1992)

Section

Artigos

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