Cultivation in vitro of Eurytrema coelomaticum
DOI:
https://doi.org/10.5433/1679-0367.1992v13n2p58Keywords:
Euryirema coelomaticum, "In vitro" cultivation, "In vitro" egg layingAbstract
Culture media Gay ABC, 109 and animate serum have been used in "in vitro" cultivation of Eurytrema coelomaticum. The criterion for recording the survival was through the motility and the observation by means of a stereoscopic microscope. The bust result was obtained by the use of sheep's serum, when it was observed that this trematode con be maintained alive for more than 1200 hours and one of the reasons for this was that ail experiments were conducted under aseptic conditions. Oats on the rale of egg laying by E. coelomaticum were observed. The overage doily rate per worm was 641 eggs. In the first 24 hours, the average was 473 eggs and it increased in 48 hours to 673 eggs per day. The average daily rate of E. coelomaticum was considered low and extremely irregular.
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