Obtenção e análise de soro heterólogo anti-receptor de linfócitos T humano para eritrócitos de carneiro
DOI:
https://doi.org/10.5433/1679-0367.1991v12n2p70Keywords:
T lymphocyte, SRBC receptor, CD2Abstract
Anti-human T lynphocyte serum specifc to the receptor for sheep erythrocytes (E) was produced by immunizing sheep with autologous erythrocytes sensitized with solubilized receptors from human lymphocytes. The anti-soluble receptors serum (anti-Rs) inhibited E-Rosotte formation, identities T lymphocytes by indirect Immunofluorescence, agglutinated the formalized E-soluble receptors complexes, and inhibited the Esoluble receptors interaction. This anti-Rs serum was also submrnited to the affinity chromatography by protein A sepharoe, and the amounts of 0,15 to 120ug of purified anti-Rs IgG were added to the lymphocyte culture. The result obtained slewed an induction of proliferative response of the normal human lymphocytes in the presence of 60 and 120ug anti-Rs lgG.
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