Prevalence of Influenza A, respiratory syncytial virus and SARS-COV-2 in patients with severe acute respiratory syndrome in Passo Fundo - RS
DOI:
https://doi.org/10.5433/1679-0367.2023v44n2p113Keywords:
Coronavirus, Real-time PCR, Respiratory viruses, PandemicAbstract
Severe acute respiratory syndrome (SARS) is characterized by symptoms of high fever, cough and dyspnea, and is in most cases related to a reduced amount of infectious agents. The objective was to assess the prevalence of respiratory viruses Influenza A (FluA), respiratory syncytial virus (RSV) and the new coronavirus (SARS-CoV-2) in patients hospitalized for SARS. Cross-sectional study, with patients hospitalized with SARS between November 2021 and May 2022. Sociodemographic and clinical data and nasopharyngeal samples were collected, which were subjected to RNA extraction and tested for positivity for Influenza A, RSV and SARS-CoV-2 using the real-time PCR technique using the SYBR Green method. 42 patients were included, 59.5% female, 57.1% elderly, 54.8% with primary education. Most patients reported previous or current smoking habits (54.8%), non-drinkers (73.8) and 83.3% of them had some comorbidity, with systemic arterial hypertension and type 2 diabetes mellitus being the most prevalent. A total of 10.5% of patients tested positive for FluA, no samples positive for RSV, and 76.3% positive for SARS-CoV-2. In the studied population, SARS with hospital injury was observed more frequently in women and the elderly, with associated comorbidities, with the new coronavirus being the most related etiological agent, which shows, although not statistically significant, that the pathogenicity of this agent and its consequences are still evident after almost 2 years of period pandemic.
Downloads
References
Bastos LS, Niquini RP, Lana RM, Villela DAM, Cruz OG, Coelho FC, et al. COVID-19 e hospitalizações por SRAG no Brasil: uma comparação até a 12a semana epidemiológica de 2020. Cad Saúde Publica. 2020;36(4). doi: 10.1590/0102-311X00070120 DOI: https://doi.org/10.1590/0102-311x00070120
Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):2000045. doi: 10.2807/1560-7917.ES.2020.25.3.2000045 DOI: https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045
Doerre A, Doblhammer G. The influence of gender on COVID-19 infections and mortality in Germany: insights from age- and gender-specific modeling of contact rates, infections, and deaths in the early phase of the pandemic. PLoS One. 2022;17(5):e0268119. doi: 10.1371/journal.pone.0268119 DOI: https://doi.org/10.1371/journal.pone.0268119
Greco DB, Tupinambás U, Fonseca M. Influenza A (H1N1): histórico, estado atual no Brasil e no mundo, perspectivas. Rev Méd Minas Gerais.2009;19(2):132-9.
Halfmann PJ, Iida S, Iwatsuki-Horimoto K, Maemura T, Kiso M, Scheaffer SM, et al. SARS-CoV-2 Omicron virus causes attenuated disease in mice and hamsters. Nature. 2022;603(7902):687-92. doi: 10.1038/s41586-022-04441-6 DOI: https://doi.org/10.1038/s41586-022-04441-6
Hansen CL, Chaves SS, Demont C, Viboud C. Mortality associated with influenza and respiratory syncytial virus in the US, 1999-2018. JAMA Netw Open. 2022;5(2):e220527. doi: 10.1001/jamanetworkopen.2022.0527 DOI: https://doi.org/10.1001/jamanetworkopen.2022.0527
He X, Hong W, Pan X, Lu G, Wei X. SARS-CoV-2 Omicron variant: characteristics and prevention. MedComm (2020). 2021;2(4):838-45. doi: 10.1002/mco2.110 DOI: https://doi.org/10.1002/mco2.110
Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res. 1996;6(10):986-94. doi: 10.1101/gr.6.10.986 DOI: https://doi.org/10.1101/gr.6.10.986
Kojima N, Roshani A, Klausner JD. Duration of COVID-19 PCR positivity for Omicron vs earlier variants. J Clin Virol Plus. 2022;2(3):100085. doi: 10.1016/j.jcvp.2022.100085 DOI: https://doi.org/10.1016/j.jcvp.2022.100085
Korsun N, Trifonova I, Dobrinov V, Madzharova I, Grigorova I, Christova I. Low prevalence of influenza viruses and predominance of A(H3N2) virus with respect to SARS-CoV-2 during the 2021-2022 season in Bulgaria. J Med Virol. 2023;95(2):e28489. doi: 10.1002/jmv.28489 DOI: https://doi.org/10.1002/jmv.28489
Magalhaes EF, Beraldo CL, Vieira ALP, Mendonça PAFS, Teixeira DV, Rocha EA, et al. Análise da prevalência de vírus respiratórios em crianças atendidas em um hospital universitário do sul de Minas Gerais. Rev Méd Minas Gerais. 2017;27:e-1870. doi: 10.5935/2238-3182.20160128 DOI: https://doi.org/10.5935/2238-3182.20170065
Marinowic DR, Zanirati G, Rodrigues FVF, Grahl MVC, Alcará AM, Machado DC, et al. A new SYBR Green real-time PCR to detect SARS-CoV-2. Sci Rep. 2021;11(1):2224. doi: 10.1038/s41598-021-81245-0 DOI: https://doi.org/10.1038/s41598-021-81245-0
Ministério da Saúde (BR). Doença pelo Novo Coronavírus-COVID-19. Bol Epidemiol Espec [Internet]. 2022 [citado 2023 ago 15];112:1-87. Disponível em: https://www.gov.br/saude/pt-br/centrais-de-conteudo/publicacoes/boletins/epidemiologicos/covid-19/2022/boletim-epidemiologico-no-112-boletim-coe-coronavirus.pdf.
Ministério da Saúde (BR). InfoGripe [Internet]. 2020 [citado 2023 ago 22]. Disponível em: https://portal.fiocruz.br/sites/portal.fiocruz.br/files/documentos/boletim_infogripe_se202012.pdf.
Ministério da Saúde (BR). Protocolo de manejo clínico de síndrome respiratória aguda grave: SRAG: versão IV. Brasília: Ministério da Saúde; 2010.
Mitchell SL, Loeffelholz MJ. Considerations regarding interpretation of positive SARS-CoV-2 molecular results with late cycle threshold values. J Clin Microbiol. 2022;60(9):e0050122. doi: 10.1128/jcm.00501-22 DOI: https://doi.org/10.1128/jcm.00501-22
Niquini RP, Lana RM, Pacheco AG, Cruz O, Coelho FC, Carvalho LM, et al. SRAG por COVID-19 no Brasil: descrição e comparação de características demográficas e comorbidades com SRAG por influenza e com a população geral. Cad Saúde Pública. 2020;36(7):e00149420. doi: 10.1590/0102-311X00149420 DOI: https://doi.org/10.1590/0102-311x00149420
Paiva KM, Hillesheim D, Rech CR, Delevatti RS, Brown RVS, Gonzáles AI, et al. Prevalence and associated factors of SARS by Covid-19 in adults and aged people with chronic cardiovascular disease. Arq Bras Cardiol. 2021;117(5):968-75. doi: 10.36660/abc.20200955 DOI: https://doi.org/10.36660/abc.20200955
Qian Z, Li Z, Peng J, Gao Q, Cai S, Xu X. Association between hypertension and prognosis of patients with COVID-19: A systematic review and meta-analysis. Clin Exp Hypertens. 2022;44(5):451-8. doi: 10.1080/10641963.2022.2071914 DOI: https://doi.org/10.1080/10641963.2022.2071914
Safadi MAP. The intriguing features of COVID-19 in children and its impact on the pandemic. J Pediatr (Rio J). 2020;96(3):265-8. doi: 10.1016/j.jped.2020.04.001 DOI: https://doi.org/10.1016/j.jped.2020.04.001
Sardinha DM, do Socorro Pompeu de Loiola R, Ferreira ALDS, de Sá CAF, Rodrigues YC, Lima KVB, et al. Risk factors associated with the severity of COVID-19 in a region of the Brazilian Amazon. Sci Rep. 2021;11(1):20569. doi: 10.1038/s41598-021-00009-y DOI: https://doi.org/10.1038/s41598-021-00009-y
Sohrabi C, Alsafi Z, O'Neill N, Khan M, Kerwan A, Al-Jabir A, et al. World Health Organization declares global emergency: a review of the 2019 novel coronavirus (COVID-19). Int J Surg. 2020;76:71-6. doi: 10.1016/j.ijsu.2020.02.034 DOI: https://doi.org/10.1016/j.ijsu.2020.02.034
Zhang T, Wu Q, Zhang Z. Probable pangolin origin of SARS-CoV-2 associated with the COVID-19 Outbreak. Curr Biol. 2020;30(7):1346-51.e2. doi: 10.1016/j.cub.2020.03.022 DOI: https://doi.org/10.1016/j.cub.2020.03.022
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Beatriz de Albuquerque Bernardi, Patrícia Marcolin, Ivana Loraine Lindemann , Renata dos Santos Rabello, Shana Ginar da Silva, Gustavo Olszanski Acrani, Jossimara Polettini
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
This Journal is licensed with a license Creative Commons Assignment-NonCommercial 4.0 International.
