Influence of vitamin C on hepatic and muscular lipoperoxidation of C57BL / 6 mice conducted on the cafeteria diet
DOI:
https://doi.org/10.5433/1679-0367.2020v41n2p239Keywords:
Ascorbic acid, Western diet, MiceAbstract
To analyze the effect of vitamin C treatment on hepatic and muscular lipoperoxidation, as well as on biochemical parameters of C57BL / 6 mice submitted to the cafeteria diet for nine weeks. Seventeen mice of the C57BL / 6 lineage, two months old, were allocated to three groups: 1) Control, 2) Cafeteria and 3) Cafeteria + Vitamin C. The biological assay was conducted for nine weeks, the animals were kept in fasting for 12 hours and after being sacrificed, blood and tissues were collected for biochemical dosages. From the samples of liver and muscle, the contents of thiobarbituric acid reactive species (TBARS) and total lipids were quantified. The livers of mice fed with a diet of coffee or not treated with vitamin C showed higher levels of TBARS compared to controls (p <0.05). The muscle TBARS content was higher in the mice of the Cafeteria + Vitamin C group compared to that found for the Cafeteria and Control animals (p <0.05). The concentrations of hepatic and muscular cholesterol were higher in the Cafeteria + Vitamin C group compared to the Control and Cafeteria groups (p <0.05). Treatment with vitamin C increased muscle lipoperoxidation, but did not influence this parameter in the liver of C57BL 6 mice fed with cafeteria diet. In addition, vitamin C increased cholesterol concentration in liver and muscle tissues, but did not change serum glycemia and serum lipids after nine weeks of treatment.
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Pereira LO, Francischi RP, Lancha Jr AH. Obesidade: Hábitos Nutricionais, Sedentarismo e Resistência à Insulina. Arq Bras Endocrinol Metab. 2003 [citado 2015 dez 9]; 47(2):111-27. Disponível em: http://www.scielo.br/pdf/abem/v47n2/a03v47n2.pdf.
Heyman L, Axling U, Blanco N, Sterner O, Holm C, Berger K. Evaluation of Beneficial Metabolic Effects of Berries in High-Fat Fed C57BL/6J Mice. J Nutr Metabolism. 2014; 2014: 1-12. doi: https://doi.org/10.1155/2014/403041.
Stipanuk MH, Caudill MA. Biochemical and Physiological Aspects of Human Nutrition. 3rd ed. Filadelphia: Saunders; 2013.
Johnston CS, Corte C, Swan PD. Marginal vitamin C status is associated with reduced fat oxidation during submaximal exercise in young adults. Nutr Metab. 2006;3:35. doi: 10.1186/1743-7075-3-35.
Eteng UN, Ibekwe HA, Amatey TE, Bassey BJ, Uboh FU, Owu DU. Effect of vitamin C on serum lipids and electrolyte profile of albino Wistar rats. Niger. J Physiol Sci. 2006;21(1-2):9-15. doi: 10.4314/njps.v21i1-2.53928.
Nascimento OV, Boleti APA, Yuyama LKO, Lima ES. Effects of diet supplementation with Camu-camu (Myrciaria dubia HBK McVaugh) fruit in a rat model of diet-induced obesity. An Acad Bras Cienc. 2013;85(1):356-63. doi: 10.1590/S0001-37652013005000001.
Garcia-Diaz DF, Campion J, Milagro FI, Paternain L, Solomon A, Martinez JA. Ascorbic acid oral treatment modifies lipolytic response and behavioural activity but not glucocorticoid metabolism in cafeteria diet-fed rats. Acta Physiol. 2009;195:449-57. doi: 10.1111/j.1748-1716.2008.01942.x
Nogueira CR, Borges F, Lameu E, Franca C, Ramalho A. Effects of supplementation of antioxidant vitamins and lipid peroxidation in critically ill patients. Nutr Hosp. 2013;28(5):1666-72. doi: 10.3305/nh.2013.28.5.6590.
Barreiros ALBS, David JM, David JP. Estresse oxidativo: relação entre geração de espécies reativas e defesa do organismo. Quim Nova. 2006; [citado 2016 jan 9]; 29(1):113-23. Disponível em: http://www.scielo.br/pdf/qn/v29n1/27866.pdf
Yuzefovych LV, Musiyenko SI, Wilson GL, Rachek LI. Mitochondrial DNA Damage and Dysfunction, and Oxidative Stress Are Associated with Endoplasmic Reticulum Stress, Protein Degradation and Apoptosis in High Fat Diet-Induced Insulin Resistance Mice. Plos One. 2013;8(1):1-8. doi: 10.1371/journal.pone.0054059.
Tranberg B, Hansen AK, Lykkesfeldt J. High-fat feeding increases hepatic vitamin C synthesis and its circulatory mobilization in mice. Eur J Nutr. 2014; [citado 2015 dez 15]; 53:1441-44. Disponível em: https://link.springer.com/article/10.1007%2Fs00394-014-0694-z
Carr AC, Vissers MCM. Synthetic or Food-Derived Vitamin C—Are They Equally Bioavailable? Nutrients. 2013;5:4282-304. doi: 10.3390/nu5114284.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8. doi: 10.1016/0003-2697(79)90738-3.
Berthoud HR, Zheng H. Modulation of taste responsiveness and food preference by obesity and weight loss. Physiol Behav. 2012;107(4):527-32. doi: 10.1016/j.physbeh.2012.04.004.
Higa TS, Spinola AV, Fonseca-Alaniz MH, Evangelista FS. Comparison between cafeteria and high-fat diets in the induction of metabolic dysfunction in mice. Int J Physiol Phatophysiol Pharmacol. 2014 [citado 2015 nov 25]; 6(1):47-54. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961101/
Ghosh C, Yang SH, Kim JG, Jeon T, Yoon BH, Lee JY et al. Zinc-chelated Vitamin C Stimulates Adipogenesis of 3T3-L1 Cells. Asian Australas J Anim Sci. 2013;26(8):1189-96. doi: 10.5713/ajas.2013.13179
Campión J, Milagro FI, Fernández D, Martínez JA. Vitamin C supplementation influences body fat mass and steroidogenesis-related genes when fed a high-fat diet. Int J Vitam Nutr Res. 2008;78(2):87-95. doi: 10.1024/0300-9831.78.2.87.
McGarry JD. Dysregulation of Fatty Acid Metabolism in the Etiology of Type 2 Diabetes. Diabetes. 2002;51:7-18. doi: 10.2337/diabetes.51.1.7.
Noeman SA, Hamooda HE, BaAlash AA. Biochemical Study of Oxidative Stress Markers in the Liver, Kidney and Heart of High Fat Diet Induced Obesity in Rats. Diabet Metab Syndr. 2011;3:17. doi: 10.1186/1758-5996-3-17.
Ellulu MS, Rahmat A, Patimah I, Khaza’ai H, Abed Y. Effect of vitamin C on inflammation and metabolic markers in hypertensive and/or diabetic obese adults: a randomized controlled trial. Drug Des Devel Ther. 2015;9:3405-12. doi: 10.2147/DDDT.S83144.
Tres Santos J, Krutzmann MW, Bierhals CC, Feksa LR. The effects of supplementation with vitamin C. RCO. 2019; [citado 2020 fev 17];1:139-63. Disponível em: https://periodicos.feevale.br/seer/index.php/revistaconhecimentoonline/article/view/1187/2275
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