Influence of vitamin C on hepatic and muscular lipoperoxidation of C57BL / 6 mice conducted on the cafeteria diet

Authors

  • Betina Fernanda Dambros Universidade Federal de Santa Catarina
  • Camila Irigonhé Ramos Universidade Federal de Pelotas http://orcid.org/0000-0001-8593-1397
  • Renata Torres Abib Universidade Federal de Pelotas
  • Sandra Costa Vale Universidade Federal de Pelotas

DOI:

https://doi.org/10.5433/1679-0367.2020v41n2p239

Keywords:

Ascorbic acid, Western diet, Mice

Abstract

To analyze the effect of vitamin C treatment on hepatic and muscular lipoperoxidation, as well as on biochemical parameters of C57BL / 6 mice submitted to the cafeteria diet for nine weeks. Seventeen mice of the C57BL / 6 lineage, two months old, were allocated to three groups: 1) Control, 2) Cafeteria and 3) Cafeteria + Vitamin C. The biological assay was conducted for nine weeks, the animals were kept in fasting for 12 hours and after being sacrificed, blood and tissues were collected for biochemical dosages. From the samples of liver and muscle, the contents of thiobarbituric acid reactive species (TBARS) and total lipids were quantified. The livers of mice fed with a diet of coffee or not treated with vitamin C showed higher levels of TBARS compared to controls (p <0.05). The muscle TBARS content was higher in the mice of the Cafeteria + Vitamin C group compared to that found for the Cafeteria and Control animals (p <0.05). The concentrations of hepatic and muscular cholesterol were higher in the Cafeteria + Vitamin C group compared to the Control and Cafeteria groups (p <0.05). Treatment with vitamin C increased muscle lipoperoxidation, but did not influence this parameter in the liver of C57BL 6 mice fed with cafeteria diet. In addition, vitamin C increased cholesterol concentration in liver and muscle tissues, but did not change serum glycemia and serum lipids after nine weeks of treatment.

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Author Biographies

Betina Fernanda Dambros, Universidade Federal de Santa Catarina

PhD student in Nutrition at the Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil.

Camila Irigonhé Ramos, Universidade Federal de Pelotas

PhD student in Sciences at the Postgraduate Program in Nursing at the Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil.

Renata Torres Abib, Universidade Federal de Pelotas

PhD in Biological Sciences (Biochemistry) from the Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil. Adjunct Professor at the Faculty of Nutrition, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.

Sandra Costa Vale, Universidade Federal de Pelotas

PhD in Biological Sciences (Physiology) from the Federal Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. Associate Professor at the Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.

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Published

2020-07-07

How to Cite

1.
Dambros BF, Ramos CI, Abib RT, Vale SC. Influence of vitamin C on hepatic and muscular lipoperoxidation of C57BL / 6 mice conducted on the cafeteria diet. Semin. Cienc. Biol. Saude [Internet]. 2020 Jul. 7 [cited 2024 Nov. 24];41(2):239-48. Available from: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/36083

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