Use of chromogenic medium and semi-nested PCR-based assay to identify Candida species
DOI:
https://doi.org/10.5433/1679-0367.2006v27n2p125Keywords:
Candida sp. Identification. CHROMagar médium. Semi-nested PCR.Abstract
In this study, the chromogenic medium CHROMagar Candida™ and semi-nested PCR-based assay (sn- PCR) were compared in relation to their capacity to identify the species of 52 clinical isolates of Candida sp. By using the chromogenic medium, 39 (75%) yeasts isolates were presumptively identified as C. albicans (n = 22), C. glabrata (n = 9), C. tropicalis (n = 5) and C. krusei (n = 3). Thirteen isolates (25%) were not distinguishable to the species level. Through the sn-PCR, that is based on the ribosomal RNA genes (5.85 – 28S) cluster amplifications, 43 (83%) isolates were identified as C. albicans (n = 24), C. glabrata (n = 11), C. tropicalis (n = 5), C. parapsilosis (n = 3) and nine isolates could not be identified to the species level. Among the 52 analyzed isolates, 34 (65.4%) were in accordance with both methods, 12 (23.1%) showed discrepant results, and 6 (11.5%) could not be identified by any of the methodologies. The results indicate that both methods are limited, but the sn-PCR is more adequate than the chromogenic medium to identify species of the Candida genus, due to the lowest number of isolates that could not be identified.
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