Karyology of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae)

Authors

  • Flávia Rangel de Souza Universidade Federal de Juiz de Fora
  • Victória Rabelo Campos Universidade Federal de Juiz de Fora
  • Ana Luiza Franco Universidade Federal de Juiz de Fora
  • Aryane Campos Reis Universidade Federal de Juiz de Fora
  • Lyderson Facio Viccini Universidade Federal de Juiz de Fora
  • Saulo Marçal de Sousa Universidade Federal de Juiz de Fora

DOI:

https://doi.org/10.5433/1679-0367.2017v38n1suplp195

Keywords:

African lily, Cytotaxonomy, Molecular cytogenetics

Abstract

The genus Agapanthus L'Héritier (Agapanthaceae) is a monocotyledonous, herbaceus and perennial plant group endemic to southern Africa. Ten species have been described for this group, in which three are considered evergreen and seven deciduous plants.  Nevertheless, Agapanthus L'Héritier taxonomy is considered very difficult once there are few unique characters and a high plasticity depending on circumstances of growing. This feature takes some authors to believe in the existence of only one species with innumerous varieties.The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically presented 2n = 30. Although the species possess large chromosomes, few karyological studies has been explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH). In addition, flow cytometry was used to determinate   DNA content and the percentage of AT / GC nitrogenous bases. Plants studied showed 2n = 30 chromosomes, ranging from 4.34 - 8.55 µm, with karyotype formulae (KF) = 10m + 5sm. The FISH technique revealed one 45S rDNA signal observed proximally to centromere of the chromosome 7, and one 5S rDNA signal observed proximally to centromere of chromosome 9. The 2C DNA content estimated for the species was 2C = 24.4pg, with 59% of AT and 41% of GC. Our data allowed important upgrade for biology and cytotaxonomy of Agapanthus africanus (L.) Hoffmanns, and will be used to future taxonomic and phylogenetics studies, as well as for the chromosome evolution of the genus.


APOIO FINANCEIRO: Fapemig, Capes, CNPq

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Author Biographies

Flávia Rangel de Souza, Universidade Federal de Juiz de Fora

Universidade Federal de Juiz de Fora, Juiz de Fora – MG

Victória Rabelo Campos, Universidade Federal de Juiz de Fora

Universidade Federal de Juiz de Fora, Juiz de Fora – MG

Ana Luiza Franco, Universidade Federal de Juiz de Fora

Universidade Federal de Juiz de Fora, Juiz de Fora – MG

Aryane Campos Reis, Universidade Federal de Juiz de Fora

Universidade Federal de Juiz de Fora, Juiz de Fora – MG

Lyderson Facio Viccini, Universidade Federal de Juiz de Fora

Universidade Federal de Juiz de Fora, Juiz de Fora – MG

Saulo Marçal de Sousa, Universidade Federal de Juiz de Fora

Universidade Federal de Juiz de Fora, Juiz de Fora – MG

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Published

2018-02-16

How to Cite

1.
Souza FR de, Campos VR, Franco AL, Reis AC, Viccini LF, Sousa SM de. Karyology of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae). Semin. Cienc. Biol. Saude [Internet]. 2018 Feb. 16 [cited 2024 Nov. 21];38(1supl):195. Available from: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/29434

Issue

Vol. 38 No. 1supl (2017): Reunião Brasileira de Citogenética e Citogenômica

Section

Resumos

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