Comparison of microdilution broth and disk diffusion for antifungal susceptibility testing against Candida spp.

Authors

  • Antônio Alexandre de Vasconcelos Júnior Universidade Federal do Ceará
  • Everardo Albuquerque Menezes Universidade Federal do Ceará
  • Francisco Afrânio Cunha Universidade Federal do Ceará
  • Maria da Conceição dos Santos Oliveira Cunha Universidade Federal do Ceará
  • Bárbara Helena Lima Braz Universidade Federal do Ceará
  • Ligya Guimarães Capelo Universidade Federal do Ceará
  • Carlla Lorena Façanha Silva Universidade Federal do Ceará

DOI:

https://doi.org/10.5433/1679-0367.2012v33n1p135

Keywords:

Microdilution broth, Diffusion Disk, Candida sp.

Abstract

The evolution of fungal resistance can be evaluated by testing susceptibility to antifungal agents. The protocols for susceptibility testing most common are Broth Microdilution (BMD), Disk Diffusion (DD) and E-test. The objective of this study was to compare the susceptibility tests conducted by the BMD and DD methods for amphotericin B and fluconazole against Candida spp. isolated in Fortaleza/CE. Were used 40 C. albicans, 50 C. tropicalis and 47 C. parapsilosis isolated from blood and urine samples of patients treated at Fortaleza General Hospital. The tests were conducted by the BMD and DD methods according to protocols M27-A3 and M44-A2 of the CLSI. These methods were compared and the percentages of agreement were calculated. For amphotericin B and fluconazole was a predominance of strains with reduced susceptibility. The percentage of agreement for the methods tested was above 97%. No serious errors were detected. The use of DD for performing antifungal susceptibility testing can be applied in routine laboratory tests, since one technique is easy, inexpensive, and reliable when compared to the BMD without compromising the results for the strains of C. albicans, C. tropicalis and C. parapsilosis.

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Author Biographies

Antônio Alexandre de Vasconcelos Júnior, Universidade Federal do Ceará

Laboratório de Microbiologia de Leveduras, Departamento de Análises Clínicas da Faculdade de Farmácia Odontologia e Enfermagem, Universidade Federal do Ceará, Brasil.

Everardo Albuquerque Menezes, Universidade Federal do Ceará

Laboratório de Microbiologia de Leveduras, Departamento de Análises Clínicas, Universidade Federal do Ceará, Brasil

Francisco Afrânio Cunha, Universidade Federal do Ceará

Laboratório de Microbiologia de Leveduras, Departamento de Análises Clínicas, Universidade Federal do Ceará, Brasil

Maria da Conceição dos Santos Oliveira Cunha, Universidade Federal do Ceará

Laboratório de Microbiologia de Leveduras, Departamento de Análises Clínicas, Universidade Federal do Ceará, Brasil

Bárbara Helena Lima Braz, Universidade Federal do Ceará

Laboratório de Microbiologia de Leveduras, Departamento de Análises Clínicas, Universidade Federal do Ceará, Brasil

Ligya Guimarães Capelo, Universidade Federal do Ceará

Laboratório de Microbiologia de Leveduras, Departamento de Análises Clínicas, Universidade Federal do Ceará, Brasil

Carlla Lorena Façanha Silva, Universidade Federal do Ceará

Curso de Química, Universidade Federal do Ceará, Brasil

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Published

2012-07-30

How to Cite

1.
Vasconcelos Júnior AA de, Menezes EA, Cunha FA, Cunha M da C dos SO, Braz BHL, Capelo LG, et al. Comparison of microdilution broth and disk diffusion for antifungal susceptibility testing against Candida spp. Semin. Cienc. Biol. Saude [Internet]. 2012 Jul. 30 [cited 2024 Nov. 21];33(1):135-42. Available from: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/10365

Issue

Vol. 33 No. 1 (2012)

Section

Artigos

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Copyright (c) 2012 Semina: Ciências Biológicas e da Saúde

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