Effect of refrigeration systems upon frozen bull sperm viability assessed by computer-assisted sperm analysis and fluorescent probes


  • Marcelo George Mungai Chacur Universidade do Oeste Paulista
  • Huberson Sanches Dias Universidade do Oeste Paulista
  • Patricia de Mello Papa Universidade do Oeste Paulista
  • Frederico Ozanam Papa Universidade Estadual Paulista
  • Cely Marini Melo-Onã Universidade Estadual Paulista




Zebu bull, Cooled semen, Frozen semen, CASA, Membrane integrity.


Sperm cryopreservation success depends upon the maintenance of spermatozoa fertility potential. Sperm cells must preserve both integrity and functionality of several cell structures. The stabilization phase must allow the exit of water from the sperm cells via osmosis. This study aimed to compare the effect of refrigeration in the commercial refrigerator (CR) and the transport/refrigeration box (TRB) upon the viability of frozen bull sperm diluted in three different extenders (A, B and C). Ten Nellore bulls, Bos taurus indicus maintained in Artificial Insemination Center were used and the spermatozoa samples was assessed for Plasma Membrane Integrity and CASA evaluation. The stabilization phase (5°C/4 hours) was performed in the CR as well as in the TRB, and then samples were exposed to nitrogen vapor during 20 minutes and then plunged into nitrogen. The statistical analysis was done using the variance analysis and the significance level was set at 5%. In the CR the post-thawing parameters for PM and ALH were higher (p < 0.05) in the extender A (glicine egg-yolk) and extender B (glicine egg-free) when compared with extender C (TRIS egg-yolk). As for BCF, STR and LIN, the parameters were higher (p < 0.05) in extender B than in C. Samples that were stabilized in the TRB presented higher post-thawing parameters (p < 0.05) for PM and LIN in extender A and extender B when compared with C. BCF and STR parameters were higher (p < 0.05) in extemder B when compared with C. Extender B samples had higher (p < 0.05) PMI when stabilized in CR. The findings in this experiment enable us to say that both CR and TRB were effective in keeping the viability of post-thawing bull semen.


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Biografia do Autor

Marcelo George Mungai Chacur, Universidade do Oeste Paulista

Prof. Universidade do Oeste Paulista, UNOESTE, Presidente Prudente, SP.

Huberson Sanches Dias, Universidade do Oeste Paulista

Pesquisador, UNOESTE, Presidente Prudente, SP.

Patricia de Mello Papa, Universidade do Oeste Paulista

Discente, UNOESTE, Presidente Prudente, SP.

Frederico Ozanam Papa, Universidade Estadual Paulista

Prof. Universidade Estadual Paulista, UNESP, Botucatu, SP.

Cely Marini Melo-Onã, Universidade Estadual Paulista

Pesquisadora, UNESP, Botucatu, SP.




Como Citar

Chacur, M. G. M., Dias, H. S., Papa, P. de M., Papa, F. O., & Melo-Onã, C. M. (2012). Effect of refrigeration systems upon frozen bull sperm viability assessed by computer-assisted sperm analysis and fluorescent probes. Semina: Ciências Agrárias, 33(5), 1923–1930. https://doi.org/10.5433/1679-0359.2012v33n5p1923




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