Production of lipase by Beauveria bassiana on broth supplemented with oily residue

Authors

  • Michele Cristina Barreto da Cruz Departmento de Bioquímca e Biotecnologia Universidade Estadual de Londrina
  • Geni da Silva Varea Editora Gerente Dep. Bioquímica e Biotecnologia Centro de Ciências Exatas Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/2316-5200.2014v3n2p9

Keywords:

Beauveria bassiana, production of lipase, oily residue

Abstract

The entomopathogenic fungus Beauveria bassiana CG432 produced an extracellular lipase on the 7th day of fermentation in Alves broth containing 0.25% Triton X-100 and Tween 80 surfactants, and 3.0% olive oil at 28°C and 200rpm. Optimal temperature and pH conditions for lipase activity were found to be range from 40 to 60°C and pH 7.5 and 8.0 using 50 mM Tris-HCl buffer, verified by a hydrolysis p-nitrophenol palmitate assay. Under these conditions, lipase was 50% stable for approximately 10h, however it was stable for up to 10 days (-18°C), refrigerated (4°C) or kept at room temperature (25°C). 

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Published

2015-03-08

Issue

Vol. 3 No. 2 (2014)

Section

Artigos Científicos

License

Os trabalhos publicados passam a ser propriedade da revista BBR: Biochemistry and Biotechnology Reports, com o trabalho simultaneamente licenciado sob a Licença Creative Commons Attribution que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação nesta revista.