Optimization of the drying parameters for an enzymatic extract, and an evaluation of the subsequent proteolytic and cellulolytic activities

Authors

  • Hamilton Cabral Universidade de São Paulo
  • Ana Claudia Rodrigues Siqueira Faculty of Pharmaceutical Sciences of Ribeirao Preto, Department of Pharmaceutical Sciences, University of Sao Paulo, Postal code 14040-903 Ribeirao Preto-SP, Brazil.
  • Nathália Gonsales Rosa Faculty of Pharmaceutical Sciences of Ribeirao Preto, Department of Pharmaceutical Sciences, University of Sao Paulo, Postal code 14040-903 Ribeirao Preto-SP, Brazil.
  • Pedro Bastos Neto Faculty of Pharmaceutical Sciences of Ribeirao Preto, Department of Pharmaceutical Sciences, University of Sao Paulo, Postal code 14040-903 Ribeirao Preto-SP, Brazil.
  • André Rodrigues Departament of Biochemistry and Microbiology, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Postal code 13506-900 Rio Claro-SP, Brazil
  • Luis Alexandre Pedro Freitas Faculty of Pharmaceutical Sciences of Ribeirao Preto, Department of Pharmaceutical Sciences, University of Sao Paulo, Postal code 14040-903 Ribeirao Preto-SP, Brazil.

DOI:

https://doi.org/10.5433/2316-5200.2013v2n1p17

Keywords:

Spray drying, solid bioprocess, Trichoderma harzianum, experimental design.

Abstract

Enzymes reduce the activation energy needed to convert a substrate into product and are therefore called biological catalysts. Peptidases are enzymes that act on the peptide bonds present in protein substrates, whereas cellulases act in various ways to degrade cellulose. Two types of cellulases are endoglucanase and exoglucanase, called in this paper carboxymethylcellulase (CMCase) and Avicelase, respectively. Trichoderma spp. can produce cellulases and peptidases due to the versatility of their saprophytic characteristics. The drying of enzymes is an interesting procedure because it provides the highest long-term stability. The enzymatic extract produced by Trichoderma harzianum was submitted to spray drying, and the stabilities of the dried enzymes were verified using casein as the substrate for a peptidase assay and carboxymethylcellulose and Avicel as substrates for a cellulolytic activities assay. The aim of this work was to select the adequate adjuvant and to evaluate the best parameters to use in drying the T. harzianum enzymatic extract, which contained peptidase, CMCase and Avicelase. We also wanted to verify the stability of these enzymes after the dried microparticles were solubilized and stored at 4°C or 25°C for 30 days.

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Published

2013-07-19

Issue

Section

Artigos Científicos