Use of Comparative Modeling in Structural Determination of Yersinia´s Phytase
DOI:
https://doi.org/10.5433/2316-5200.2013v2n1p25Keywords:
Homology modeling, computational biotechnology, bioinformaticsAbstract
Phytases (EC 3.1.3.8) when mixed into animal feed increases the nutritional quality by increasing the bioavailability of phosphate. However, the use of phytase is restricted due to its low bioavailability by gastric degradation. In order to develop acid-resistant phytase, a model of Yersinia phytase was built by comparative modeling. Initially, a search for templates structures following by build model was carried out using SWISS MODEL software. The built model was evaluated in relation of backbone stereochemistry by PROCHEK. Finally, the model was refinement by molecular dynamics simulations for 8 nanoseconds through AMBER software. As a result, the model has the same structural characteristics of template, Escherichia coli phytase, PDB: 1DKQ. This model will permit studies of directed mutagenesis in silico, allowing the development of phytases with more appropriate physicochemical properties.Downloads
Published
2013-07-23
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Section
Artigos Científicos