Extraction of ?-galactosidase from Kluyveromyces marxianus CCT 7082 by ultrasonic method

Abstract

The B-galactosidase is an intracellular enzyme obtained from the yeast Kluyveromyces marxianus and its role is hydrolyse lactose and therefore, is important in the dairy industry. However, for their effective use is necessary to better understand the extraction methods available. The ultrasonic disruption method has been applied as a separation step of intracellular products on laboratory scale and consists of ultrasonic waves dissipation in the solution through cavitation bubbles and produce velocity gradient that creates a force capable of breaking the cells. The aim of this study was to evaluate the rupture ultrasonic extraction of the enzyme B-galactosidase obtained from the yeast Kluyveromyces marxianus CCT 7082 compared with abrasion method using glass beads. The method using ultrasonic ruptor (150 W) with an intermediate tip (70% power) was effective for cell disruption of yeast, showing high enzyme activity and yield, similar values to the extraction method using abrasive glass beads.