Repetitive DNA distribution in Agave (Asparagaceae) bimodal karyotype

Autores

  • Lamonier Chaves Ramos Universidade Federal Rural de Pernambuco
  • Mariana Alejandra Báez Universidade Federal de Pernambuco
  • Andreas Houben Leibniz Institute of Plant Genetics and Crop Plant Research
  • José Jaime Vasconcelos Cavalcanti Empresa Brasileira de Pesquisa Agropecuária
  • Reginaldo de Carvalho Universidade Federal Rural de Pernambuco
  • Andrea Pedrosa Harand Universidade Federal de Pernambuco

DOI:

https://doi.org/10.5433/1679-0367.2017v38n1suplp233

Palavras-chave:

Bimodal karyotype, Transposable elements, Satellite DNA

Resumo

The genus Agave L. comprises about 300 species and is characterized by a bimodal karyotype with basic chromosome number x = 30 (5L and 25S) and ploidy levels ranging from diploids to hexaploids. Species of this genus have low heterochromatin content, visualized as CMA+/DAPI- bands, and one 5S and 35S ribosomal DNA sites per haploid genome. However, in silico analysis of the repetitive fraction of the Agave hybrid 11648 (2n = 2x = 60, 8.35 Mpb) revealed that ~ 67.6% of its genome is composed of different repetitive DNA families. Among the most abundant elements are Maximus, TAR and Tork (Copia), and Athila, Chromovirus and Ogre (Gypsy) LTR-retrotransponsons, as well as, a single family of satellite DNA. In order to investigate the repetitive DNA distribution in this bimodal karyotype, these seven elements were labeled by nick translation and hybridized in situ to the hybrid 11648 metaphases. The satellite DNA was located at centromeric regions of all chromosomes, however a subset of the small chromosomes had stronger signals. All transposable elements showed a dispersed distribution, mostly non-uniform along chromosomes arms. Athila and Maximus lineages showed stronger labeling of interstitial and distal regions of the long arms of large chromosomes, while Tork and especially TAR labeled more intensely the proximal regions. Small chromosomes showed variable labeling with all repetitive elements, generally weaker than large chromosomes. Together, these data revealed that different LTR retrotransposon lineages accumulated differentially, and usually more abundantly, in the large chromosomes, whereas the centromeric satellite DNA showed a quantitative variation between chromosomes, but not related to the bimodal condition of this karyotype.

Financial support: CAPES, CNPq.

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Biografia do Autor

Lamonier Chaves Ramos, Universidade Federal Rural de Pernambuco

Universidade Federal Rural de Pernambuco, Recife – PE

Mariana Alejandra Báez, Universidade Federal de Pernambuco

Laboratório de Citogenética e Evolução Vegetal, Departamento de Botânica, Centro de Biociências, Universidade Federal de Pernambuco, Recife - PE

Andreas Houben, Leibniz Institute of Plant Genetics and Crop Plant Research

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

José Jaime Vasconcelos Cavalcanti, Empresa Brasileira de Pesquisa Agropecuária

Empresa Brasileira de Pesquisa Agropecuária – Embrapa Algodão, Campina Grande - PB

Reginaldo de Carvalho, Universidade Federal Rural de Pernambuco

Universidade Federal Rural de Pernambuco, Recife – PE

Andrea Pedrosa Harand, Universidade Federal de Pernambuco

Laboratório de Citogenética e Evolução Vegetal, Departamento de Botânica, Centro de Biociências, Universidade Federal de Pernambuco, Recife - PE

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Publicado

2018-02-16

Como Citar

1.
Ramos LC, Báez MA, Houben A, Cavalcanti JJV, Carvalho R de, Pedrosa Harand A. Repetitive DNA distribution in Agave (Asparagaceae) bimodal karyotype. Semin. Cienc. Biol. Saude [Internet]. 16º de fevereiro de 2018 [citado 14º de dezembro de 2024];38(1supl):233. Disponível em: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/29436