A validation of Hemo_Vet, a portable hemoglobinometer, in dogs

Autores

  • Ana Carolina Pereira Universidade Estadual de Londrina
  • Thiago Guerreiro Teixeira Universidade de Franca
  • Vanessa Andrea Pincelli Universidade Estadual de Londrina
  • Mariana Menezes Bochio Universidade Estadual de Londrina
  • Karina Keller Marques da Costa Flaiban Universidade Estadual de Londrina
  • Patrícia Mendes Pereira Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2014v35n4p1935

Palavras-chave:

Dog, Hemoglobin, Hemoglobinometer, Packed cell volume.

Resumo

Determinations of hemoglobin (Hb) levels and packed cell volumes (PCV) can be performed by various methods, including rapid testing using a portable hemoglobinometer. As a simple method that requires small blood samples and provides results in seconds, the utilization of portable hemoglobinometers has become increasingly widespread. The aim of this study was to evaluate the performance of the Hemo_ Vet, a portable hemoglobinometer, in Hb measurements and PCV determinations in dogs. A total of 100 blood samples were used to compare the Hb and PCV values provided by the Hemo_Vet with those obtained using an automatic hematology analyzer (BC2800VET) and the microhematocrit technique. The Bland-Altman method was used for data analysis. Bland-Altman analysis revealed a high level of agreement between the Hb values obtained from the BC2800VET and those obtained using the Hemo_ Vet. The bias was close to zero (0.17), and although it was statistically significant (p = 0.0003), it unlikely would result in any clinically significant effects. The differences in the PCV values generated using the hemoglobinometer and the BC2800VET technique and those generated using the hemoglobinometer and microhematocrit technique exhibited a bias of 7.80 and 2.80, respectively, indicating a high degree of variability between these methods. In addition, the hemoglobinometer was not able to determine PCV values below 18% and above 59%. The precision, standard deviation and coefficients of variation for the device all showed low variation. These results indicate that the hemoglobinometer exhibits good precision and fulfills its primary purpose, which is to measure Hb levels. However, this device was less effective in determining PCV values, where it exhibited high level of variation in both methods of comparison. These results revealed the need to change the conversion factor for estimations of PCV values from 2.94 to 3.54 for dogs.

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Biografia do Autor

Ana Carolina Pereira, Universidade Estadual de Londrina

Residente em Cirurgia de Pequenos Animais pela Universidade Estadual de Londrina,UEL, Londrina, PR. Brasil.

Thiago Guerreiro Teixeira, Universidade de Franca

Residente em Cirurgia de Pequenos Animais pela Universidade de Franca, UNIFRAN, Franca, SP, Brasil.

Vanessa Andrea Pincelli, Universidade Estadual de Londrina

Mestre em Ciência Animal, UEL, Londrina, PR, Brasil.

Mariana Menezes Bochio, Universidade Estadual de Londrina

Mestre em Ciência Animal, UEL, Londrina, PR, Brasil.

Karina Keller Marques da Costa Flaiban, Universidade Estadual de Londrina

Profª Drª do Deptº de Medicina Veterinária Preventiva, UEL, Londrina, PR, Brasil.

Patrícia Mendes Pereira, Universidade Estadual de Londrina

Profª Drª do Deptº de Clínicas Veterinárias, UEL, Londrina, PR, Brasil.

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Publicado

2014-08-27

Como Citar

Pereira, A. C., Teixeira, T. G., Pincelli, V. A., Bochio, M. M., Flaiban, K. K. M. da C., & Pereira, P. M. (2014). A validation of Hemo_Vet, a portable hemoglobinometer, in dogs. Semina: Ciências Agrárias, 35(4), 1935–1946. https://doi.org/10.5433/1679-0359.2014v35n4p1935

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