@article{Yoshihara_Minho_Cardim_Tabacow_Yamamura_2014, title={In vitro ovicidal and larvicidal activity of condensed tannins on gastrointestinal nematode infestations in sheep (Ovis aries)}, volume={35}, url={https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16703}, DOI={10.5433/1679-0359.2014v35n6p3173}, abstractNote={<p>Infestations of gastrointestinal parasitic nematodes in sheep result in significant costs to farmers. These infestations are controlled using synthetic anthelmintic treatments, which can result in the development of anthelmintic resistance in nematodes. The use of plants rich in condensed tannins (CTs) is a promising alternative for controlling infestations of harmful parasites in sheep, and could allow reduction of the chemical products used. This study investigated the <em>in vitro </em>effect of CTs from <em>Acacia mearnsii </em>extract (AE) on egg hatching and motility of third-stage larvae. Egg-hatching rate was measured after incubation with extracts for 48 h at 27 °C. The egg hatch test was performed with dilutions of 0.09, 0.19, 0.39, 0.78, 1.56, 3.12, 6.25, 12.5, 25, 50, and 100 mg mL-1. Distilled water was used as the negative control. The corresponding egg hatching inhibition percentages were 22.3, 32.3, 39.2, 49.1, 56.7, 59.0, 62.3, 77.3, 92.7, 98.3, and 100%. The concentration required to inhibit egg hatching in 50% of eggs (LC50) was 2.85 mg mL-1. The inhibition achieved with the negative control was 7.06%. A larval migration inhibition test was carried out after incubation with the extracts for 48 h at 27 oC, with AE and distilled water used in dilutions of 3.12, 6.25, 12.5, 25, 50, and 100 mg mL-1. The corresponding percentages of migration inhibition were 16.5, 37.0, 56.3, 79.4, 91.8, and 97.1%. The concentration required to inhibit migration of 50% of larvae (LC50) was 12.45 mg mL-1. The inhibition achieved with the negative control was 8.53%. The <em>in vitro </em>ovicidal and larvicidal activity of CTs from AE indicate the anthelmintic effect of AE, suggesting the potential of CT extracts to be used as alternatives for controlling gastrointestinal nematode infestations in small ruminants.</p>}, number={6}, journal={Semina: Ciências Agrárias}, author={Yoshihara, Eidi and Minho, Alessandro Pelegrine and Cardim, Sérgio Tosi and Tabacow, Victor Bittencourt Dutra and Yamamura, Milton Hissashi}, year={2014}, month={dez.}, pages={3173–3180} }