@article{Almeida_Giuffrida_Andrade_Chaves_2014, title={Muscoid Diptera as potential vectors of bacterial agents on dairy farms in the northern region of Paraná, Brazil}, volume={35}, url={https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15580}, DOI={10.5433/1679-0359.2014v35n6p3127}, abstractNote={<p class="Pa7">The aim of this study was to determine the isolation frequency and microbial sensitivity profiles of <em>Escherichia coli, Salmonella </em>spp., and <em>Staphylococcus </em>spp. isolated from synanthropic flies captured in entomological traps placed near the milking parlors of 30 dairy farms located in Northern Paraná, Brazil. In total, 192 flies were captured, and the isolation frequencies of muscids (21/30 = 70%) and califorids (27/30 = 90%) were significantly higher than that of sarcophagids (7/30 = 23.3%). Bacteriological tests on the internal contents and external surfaces of the flies showed that <em>E. coli </em>was present only in muscids (14.3%) and caliphorids (33.3%). <em>Salmonella </em>spp. was isolated from 9.5% of the muscids, 7.4% of the caliphorids, and 14.29% of the sarcophagids. <em>Staphylococcus </em>spp. was isolated from 28.5% of the muscids and 29.6% of the caliphorids. <em>E. coli </em>isolation was more frequent in flies captured on farms at which domestic chickens were housed near the milking parlors (p = 0.031) and which did not use sugarcane for animal feed (p = 0.042). Two out of 27 (7.4%) <em>Staphylococcus </em>spp. strains presented a phenotype of coagulase enzyme production. Ceftriaxone, ciprofloxacin, enrofloxacin, and gentamicin were the most effective antibiotics against <em>E. coli </em>and <em>Salmonella </em>spp. Tetracycline was the least effective antibiotic against the isolates. The <em>Staphylococcus </em>spp. strains isolated did not show the phenotype of oxacillin resistance. We conclude that regional flies are potential mechanical vectors of microbial agents able to cause enteritis in goats, mastitis in cows, and contamination of milk products for human consumption.</p>}, number={6}, journal={Semina: Ciências Agrárias}, author={Almeida, Josaine Leila and Giuffrida, Rogerio and Andrade, Ricardo Augusto Pereira and Chaves, Marina Platzeck}, year={2014}, month={dez.}, pages={3127–3138} }