@article{Santarém_Exposto_Ramires_Bin_Rubinsky-Elefant_Laposy_Nogueira_2014, title={Detection of larvae of Toxocara canis in milk: an experimental study in rabbits}, volume={35}, url={https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14396}, DOI={10.5433/1679-0359.2014v35n1p357}, abstractNote={<p class="Pa7">Toxocariasis, caused most commonly by <em>Toxocara canis</em>, is an important cosmopolitan zoonosis. Paratenic hosts have been employed to provide knowledge regard to the transmission of toxocariasis. Transmammary transmission in murine experimentally infected was observed based on the recovery of larvae from the tissue. The aim of this study was to evaluate the possibility of transmammary transmission of <em>Toxocara canis </em>in rabbits by detecting larvae directly in milk. Seventeen sexually mature virgin white New Zealand female rabbits were divided into two groups. Twelve animals were orally inoculated with 1,000 <em>T. canis </em>embryonated eggs (infected group), and five animals remained uninfected (control group). One month following the infection, the females were mated. Manual collection of 500 ?L of milk from each rabbit was performed on days +7, +14 and +21 of lactation for three consecutive lactations. The recovery of larvae was determined via a centrifuge-sedimentation technique using ether and formalin solutions. ELISA test was run to confirm the production of anti-<em>T. canis </em>antibodies (IgG) by infected rabbits. The presence of larvae was observed in milk samples from 5 (41.7%) of the 12 infected rabbits<em>. </em>The total number of recovered larvae was 20, ranging from 1 to 4 larvae per lactation/rabbit. Larvae were recovered exclusively on days 7 and 14 of lactation. Recovery was verified in different lactations. No significant difference was observed with respect to the number of larvae either in the same lactation period or in different lactation periods. Anti-<em>T. canis </em>antibodies were detected in all infected rabbits. In conclusion, the presence of larvae in rabbit milk samples suggests the possibility of galactogenic transmission of <em>T. canis </em>in paratenic hosts. Moreover, the technique employed in this study allows for the recovery of larvae directly from milk.</p>}, number={1}, journal={Semina: Ciências Agrárias}, author={Santarém, Vamilton Alvares and Exposto, Célia Fátima Silva and Ramires, Lívia Magosso and Bin, Lundia Luara Cavalcante and Rubinsky-Elefant, Guita and Laposy, Cecília Braga and Nogueira, Rosa Maria Barilli}, year={2014}, month={fev.}, pages={357–364} }